ID   PRCA_NOSS1              Reviewed;         662 AA.
AC   Q59149;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 2.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Calcium-dependent protease;
DE            EC=3.4.21.-;
DE   AltName: Full=Trypsin;
DE   Flags: Precursor;
GN   Name=prcA; OrderedLocusNames=alr1381;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-608.
RA   Maldener I., Gerdes C., Lockau W.;
RL   Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Degrades phycobiliproteins in vitro. Has a substrate
CC       specificity similar to that of trypsin (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; BA000019; BAB73338.1; -; Genomic_DNA.
DR   EMBL; X63439; CAA45036.1; -; Genomic_DNA.
DR   PIR; AB1979; AB1979.
DR   RefSeq; WP_010995553.1; NZ_RSCN01000029.1.
DR   AlphaFoldDB; Q59149; -.
DR   SMR; Q59149; -.
DR   STRING; 103690.17130728; -.
DR   MEROPS; S08.079; -.
DR   EnsemblBacteria; BAB73338; BAB73338; BAB73338.
DR   KEGG; ana:alr1381; -.
DR   eggNOG; COG1404; Bacteria.
DR   eggNOG; COG4935; Bacteria.
DR   OMA; LCHPELV; -.
DR   OrthoDB; 923655at2; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07498; Peptidases_S8_15; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR002884; P_dom.
DR   InterPro; IPR034054; Pep_S8_PrcA.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR   InterPro; IPR022398; Peptidase_S8_His-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   Pfam; PF01483; P_proprotein; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51829; P_HOMO_B; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00136; SUBTILASE_ASP; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   3: Inferred from homology;
KW   Calcium; Cytoplasm; Hydrolase; Protease; Reference proteome;
KW   Serine protease; Zymogen.
FT   PROPEP          1..?
FT                   /id="PRO_0000027130"
FT   CHAIN           ?..662
FT                   /note="Calcium-dependent protease"
FT                   /id="PRO_0000027131"
FT   DOMAIN          196..529
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   DOMAIN          535..662
FT                   /note="P/Homo B"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01173"
FT   ACT_SITE        233
FT                   /note="Charge relay system"
FT   ACT_SITE        270
FT                   /note="Charge relay system"
FT   ACT_SITE        466
FT                   /note="Charge relay system"
SQ   SEQUENCE   662 AA;  71527 MW;  2DA16E5146531CA7 CRC64;
     MVHVRYGGQN GEQYELAISE NHIVVRTESR SSLISDRPFE AAPVSSQARN ILNQFELSTR
     FSQAGVEVLH VKEPSQDGAL RDTAREILNQ EPEVQFAGRV LIDPVSQQPI VYTENLFVKF
     GHEEDVSFCQ EILGRYGLTI KRQLEYARNA YFVSAPPNTG LAIFDIAERL LNEESVELCH
     PELVREFRQR QAFSQQWHLK ETTIGGKTIK AHANVEAAWK LSDGTGTIIA IIDDGVDVDH
     EEFRSSGKIV APRDVTRKTN FPTPGNRDNH GTACAGVACG NGNFGASGVA PGAKLMPIRF
     VSALGSQDEA DSFVWAAQNG ADVISCSWGP PDGTWWDDKD PLHKQKVPLP DSTRLAMDYA
     INKGRNGKGC VILFAAGNGN ESVDNDGYAS YEKVIAVAAC NDFGTRSAYS DFGQAVWCAF
     PSNNGNPSQT PGIWTADRSG VVGYNSGSTN LGDQAGNYTN SFGGTSSACP GAAGVAALIL
     SRNPNLRWDE VRDIIKRSCD RIDPVGGNYN AEGRSPFYGY GRINALKAVE LALPAQPEPV
     SIFTAVQDVP INDLQTSQLS LAIANTNPIK SIKVTVDIEH TYIGDLIVSL NPPGECGVLP
     IILHDRKGGG ADDIKQTYDE VSTPGLTALK GKIPQGTWTL EVADKAQADT GKIRSLTIEL
     GF