PRCD_MOUSE
ID PRCD_MOUSE Reviewed; 53 AA.
AC Q00LT2;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Photoreceptor disk component PRCD {ECO:0000312|MGI:MGI:3649529};
DE AltName: Full=Progressive rod-cone degeneration protein homolog {ECO:0000250|UniProtKB:Q00LT1};
GN Name=Prcd {ECO:0000312|MGI:MGI:3649529}; Synonyms=Gm11744;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16938425; DOI=10.1016/j.ygeno.2006.07.007;
RA Zangerl B., Goldstein O., Philp A.R., Lindauer S.J.P., Pearce-Kelling S.E.,
RA Mullins R.F., Graphodatsky A.S., Ripoll D., Felix J.S., Stone E.M.,
RA Acland G.M., Aguirre G.D.;
RT "Identical mutation in a novel retinal gene causes progressive rod-cone
RT degeneration in dogs and retinitis pigmentosa in humans.";
RL Genomics 88:551-563(2006).
RN [2]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23672200; DOI=10.1021/pr4003678;
RA Skiba N.P., Spencer W.J., Salinas R.Y., Lieu E.C., Thompson J.W.,
RA Arshavsky V.Y.;
RT "Proteomic identification of unique photoreceptor disc components reveals
RT the presence of PRCD, a protein linked to retinal degeneration.";
RL J. Proteome Res. 12:3010-3018(2013).
RN [3]
RP INTERACTION WITH RHO, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP PALMITOYLATION AT CYS-2, AND MUTAGENESIS OF CYS-2.
RX PubMed=27509380; DOI=10.1021/acs.biochem.6b00489;
RA Spencer W.J., Pearring J.N., Salinas R.Y., Loiselle D.R., Skiba N.P.,
RA Arshavsky V.Y.;
RT "Progressive Rod-Cone Degeneration (PRCD) Protein Requires N-Terminal S-
RT Acylation and Rhodopsin Binding for Photoreceptor Outer Segment
RT Localization and Maintaining Intracellular Stability.";
RL Biochemistry 55:5028-5037(2016).
RN [4]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=27613864; DOI=10.1074/jbc.m116.742767;
RA Murphy J., Kolandaivelu S.;
RT "Palmitoylation of Progressive Rod-Cone Degeneration (PRCD) Regulates
RT Protein Stability and Localization.";
RL J. Biol. Chem. 291:23036-23046(2016).
CC -!- FUNCTION: Involved in vision. {ECO:0000250|UniProtKB:Q00LT1}.
CC -!- SUBUNIT: Interacts with RHO/rhodopsin; the interaction promotes PRCD
CC stability. {ECO:0000269|PubMed:27509380}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium, photoreceptor outer
CC segment {ECO:0000269|PubMed:23672200, ECO:0000269|PubMed:27509380,
CC ECO:0000269|PubMed:27613864}. Membrane {ECO:0000269|PubMed:27509380,
CC ECO:0000269|PubMed:27613864}; Lipid-anchor
CC {ECO:0000269|PubMed:27509380}; Cytoplasmic side
CC {ECO:0000269|PubMed:27509380}. Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:Q00LT1}. Golgi apparatus
CC {ECO:0000250|UniProtKB:Q00LT1}. Note=Localizes to photoreceptor disk
CC membranes in the photoreceptor outer segment.
CC {ECO:0000269|PubMed:27509380}.
CC -!- TISSUE SPECIFICITY: Expressed in retina, where it localizes to both rod
CC and cone photoreceptors (at protein level).
CC {ECO:0000269|PubMed:23672200, ECO:0000269|PubMed:27509380,
CC ECO:0000269|PubMed:27613864}.
CC -!- PTM: Palmitoylated at Cys-2 (PubMed:27509380). Palmitoylation is
CC essential for protein stability and trafficking to the photoreceptor
CC outer segment, but does not appear to be essential for membrane
CC localization (PubMed:27509380). Probably palmitoylated by ZDHHC3 (By
CC similarity). {ECO:0000250|UniProtKB:Q00LT1,
CC ECO:0000269|PubMed:27509380}.
CC -!- PTM: Phosphorylated. {ECO:0000250|UniProtKB:E1B7R9}.
CC -!- SIMILARITY: Belongs to the PRCD family. {ECO:0000305}.
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DR EMBL; DQ390337; ABD17428.1; -; mRNA.
DR CCDS; CCDS48987.1; -.
DR RefSeq; NP_001156790.1; NM_001163318.1.
DR AlphaFoldDB; Q00LT2; -.
DR SwissPalm; Q00LT2; -.
DR PaxDb; Q00LT2; -.
DR PRIDE; Q00LT2; -.
DR Ensembl; ENSMUST00000116318; ENSMUSP00000112020; ENSMUSG00000075410.
DR Ensembl; ENSMUST00000148484; ENSMUSP00000119671; ENSMUSG00000075410.
DR GeneID; 100038570; -.
DR KEGG; mmu:100038570; -.
DR UCSC; uc007mlx.2; mouse.
DR CTD; 768206; -.
DR MGI; MGI:3649529; Prcd.
DR VEuPathDB; HostDB:ENSMUSG00000075410; -.
DR GeneTree; ENSGT00900000143652; -.
DR HOGENOM; CLU_3175255_0_0_1; -.
DR InParanoid; Q00LT2; -.
DR OMA; CRRRFAN; -.
DR OrthoDB; 1638622at2759; -.
DR BioGRID-ORCS; 100038570; 2 hits in 59 CRISPR screens.
DR ChiTaRS; Prcd; mouse.
DR PRO; PR:Q00LT2; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q00LT2; protein.
DR Bgee; ENSMUSG00000075410; Expressed in retinal neural layer and 94 other tissues.
DR ExpressionAtlas; Q00LT2; baseline and differential.
DR Genevisible; Q00LT2; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0001750; C:photoreceptor outer segment; IDA:HGNC.
DR GO; GO:0042622; C:photoreceptor outer segment membrane; IDA:UniProtKB.
DR GO; GO:0002046; F:opsin binding; IPI:UniProtKB.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR027937; PRCD.
DR PANTHER; PTHR38501; PTHR38501; 1.
DR Pfam; PF15201; Rod_cone_degen; 1.
PE 1: Evidence at protein level;
KW Cell projection; Endoplasmic reticulum; Golgi apparatus; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Sensory transduction; Vision.
FT CHAIN 1..53
FT /note="Photoreceptor disk component PRCD"
FT /evidence="ECO:0000255"
FT /id="PRO_0000280354"
FT REGION 24..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000269|PubMed:27509380,
FT ECO:0000305|PubMed:27613864"
FT MUTAGEN 2
FT /note="C->Y: Loss of N-terminal lipidation. Fails to
FT localize to the photoreceptor outer segment."
FT /evidence="ECO:0000269|PubMed:27509380"
SQ SEQUENCE 53 AA; 5931 MW; DE36C6262A6BC42C CRC64;
MCTTLFLFSL AMLWRRRFTN RVEPEPSRVD GTVVGSGSDT DLQSTGREKG PVK
