PRCMB_TRYCC
ID PRCMB_TRYCC Reviewed; 354 AA.
AC Q868H8; Q4CKP0; Q4CN62;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Proline racemase B;
DE EC=5.1.1.4;
DE AltName: Full=TcPA45-B;
DE AltName: Full=TcPRACB;
GN Name=PA45-B; ORFNames=Tc00.1047053430737.10, Tc00.1047053509935.29;
OS Trypanosoma cruzi (strain CL Brener).
OC Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum.
OX NCBI_TaxID=353153;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], BIOPHYSICOCHEMICAL PROPERTIES, AND
RP SUBCELLULAR LOCATION.
RC STRAIN=CL Brener;
RX PubMed=12735293; DOI=10.1074/jbc.m210830200;
RA Chamond N., Gregoire C., Coatnoan N., Rougeot C., Freitas-Junior L.H.,
RA da Silveira J.F., Degrave W.M., Minoprio P.;
RT "Biochemical characterization of proline racemases from the human protozoan
RT parasite Trypanosoma cruzi and definition of putative protein signatures.";
RL J. Biol. Chem. 278:15484-15494(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CL Brener;
RX PubMed=16020725; DOI=10.1126/science.1112631;
RA El-Sayed N.M.A., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G.,
RA Tran A.-N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G.,
RA Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B.,
RA Anupama A., Arner E., Aslund L., Attipoe P., Bontempi E., Bringaud F.,
RA Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H.,
RA da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G.,
RA Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y.,
RA Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J.,
RA Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y.,
RA Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M.,
RA Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L.,
RA Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J.,
RA Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C.,
RA Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D.,
RA Andersson B.;
RT "The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas
RT disease.";
RL Science 309:409-415(2005).
RN [3]
RP FUNCTION.
RC STRAIN=CL Brener;
RX PubMed=16164548; DOI=10.1111/j.1365-2958.2005.04808.x;
RA Chamond N., Goytia M., Coatnoan N., Barale J.-C., Cosson A., Degrave W.M.,
RA Minoprio P.;
RT "Trypanosoma cruzi proline racemases are involved in parasite
RT differentiation and infectivity.";
RL Mol. Microbiol. 58:46-60(2005).
CC -!- FUNCTION: Catalyzes the interconversion of L- and D-proline.
CC {ECO:0000269|PubMed:16164548}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-proline = D-proline; Xref=Rhea:RHEA:10680,
CC ChEBI:CHEBI:57726, ChEBI:CHEBI:60039; EC=5.1.1.4;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=75 mM for L-proline {ECO:0000269|PubMed:12735293};
CC Vmax=0.2 mmol/sec/ug enzyme {ECO:0000269|PubMed:12735293};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12735293}.
CC -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAN80842.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EAN81716.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY140947; AAN28010.1; -; Genomic_DNA.
DR EMBL; AAHK01003090; EAN81716.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AAHK01006911; EAN80842.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_802288.1; XM_797195.1.
DR RefSeq; XP_803162.1; XM_798069.1.
DR AlphaFoldDB; Q868H8; -.
DR SMR; Q868H8; -.
DR STRING; 353153.Q868H8; -.
DR EnsemblProtists; EAN80842; EAN80842; Tc00.1047053430737.10.
DR EnsemblProtists; EAN81716; EAN81716; Tc00.1047053509935.29.
DR GeneID; 3531448; -.
DR GeneID; 3532736; -.
DR KEGG; tcr:430737.10; -.
DR KEGG; tcr:509935.29; -.
DR OrthoDB; 894373at2759; -.
DR BRENDA; 5.1.1.4; 6524.
DR SABIO-RK; Q868H8; -.
DR Proteomes; UP000002296; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018112; F:proline racemase activity; IEA:UniProtKB-EC.
DR InterPro; IPR008794; Pro_racemase_fam.
DR PANTHER; PTHR33442; PTHR33442; 1.
DR Pfam; PF05544; Pro_racemase; 1.
DR PIRSF; PIRSF029792; Pro_racemase; 1.
DR SFLD; SFLDS00028; Proline_Racemase; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Isomerase; Reference proteome.
FT CHAIN 1..354
FT /note="Proline racemase B"
FT /id="PRO_0000288953"
FT ACT_SITE 91
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 261
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 92..93
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 257
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 262..263
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 354 AA; 38465 MW; F992870423341B97 CRC64;
MRFKKSLTCI DMHTEGEAAR IVTSGLPHIP GSNMAEKKAY LQENMDYLRR GIMLEPRGHD
DMFGAFLFDP IEEGADLGIV FMDTGGYLNM CGHNSIAAVT AAVETGILSV PAKATNVPVV
LDTPAGLVRG TAHLQSGTES EVSNASIINV PSFLYQQDVV IVLPKPYGEV RVDIAFGGNF
FAIVPAEHLG IDISVQNLSR LQEAGELLRT EINRSVKVQH PQLPHINTVD CVEIYGNATN
PEAKYKNVVI FGNRQADRSP CGTGTSAKMA TLYAKGQLRI GETFVYESIL GSLFQGRVLG
EERIPGVKVP VTKDAEEGML VVTTEITGKA FIMGFNTMLF DPTDPFLNGF TLKR
