PRD1_ARATH
ID PRD1_ARATH Reviewed; 1330 AA.
AC O23277; A8QW54; F4JUM6;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 3.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Protein PUTATIVE RECOMBINATION INITIATION DEFECT 1 {ECO:0000303|PubMed:17762870};
DE Short=AtPRD1 {ECO:0000303|PubMed:17762870};
DE Short=Protein PRD1 {ECO:0000303|PubMed:17762870};
GN Name=PRD1 {ECO:0000303|PubMed:17762870};
GN OrderedLocusNames=At4g14180 {ECO:0000312|Araport:AT4G14180};
GN ORFNames=dl3130w {ECO:0000312|EMBL:CAB46002.1},
GN FCAALL.114 {ECO:0000312|EMBL:CAB78460.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY,
RP SUBCELLULAR LOCATION, INTERACTION WITH SPO11-1 AND SPO11-2, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Wassilewskija;
RX PubMed=17762870; DOI=10.1038/sj.emboj.7601815;
RA De Muyt A., Vezon D., Gendrot G., Gallois J.-L., Stevens R., Grelon M.;
RT "AtPRD1 is required for meiotic double strand break formation in
RT Arabidopsis thaliana.";
RL EMBO J. 26:4126-4137(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19763177; DOI=10.1371/journal.pgen.1000654;
RA De Muyt A., Pereira L., Vezon D., Chelysheva L., Gendrot G., Chambon A.,
RA Laine-Choinard S., Pelletier G., Mercier R., Nogue F., Grelon M.;
RT "A high throughput genetic screen identifies new early meiotic
RT recombination functions in Arabidopsis thaliana.";
RL PLoS Genet. 5:E1000654-E1000654(2009).
RN [6]
RP INTERACTION WITH DFO; PRD3; SPO11-1; SPO11-2 AND MTOPVIB.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=28855712; DOI=10.1038/s41598-017-10270-9;
RA Tang Y., Yin Z., Zeng Y., Zhang Q., Chen L., He Y., Lu P., Ye D., Zhang X.;
RT "MTOPVIB interacts with AtPRD1 and plays important roles in formation of
RT meiotic DNA double-strand breaks in Arabidopsis.";
RL Sci. Rep. 7:10007-10007(2017).
CC -!- FUNCTION: Involved in DNA cleavage that forms the double-strand breaks
CC (DSB) that initiate meiotic recombination.
CC {ECO:0000269|PubMed:17762870, ECO:0000269|PubMed:19763177}.
CC -!- SUBUNIT: Interacts with SPO11-1 (PubMed:17762870, PubMed:28855712).
CC According to PubMed:28855712, may interact with SPO11-2; this is in
CC contradiction with PubMed:9461215 which claims that it seems to not
CC interact with SPO11-2 (PubMed:28855712, PubMed:17762870). Binds to DFO,
CC PRD3 and MTOPVIB (PubMed:28855712). Facilitates an interaction between
CC PRD3 and DFO (PubMed:28855712). {ECO:0000269|PubMed:17762870,
CC ECO:0000269|PubMed:28855712}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17762870}.
CC -!- TISSUE SPECIFICITY: Expressed in flower buds.
CC {ECO:0000269|PubMed:17762870}.
CC -!- DOMAIN: The N-terminal domain (1-802) but not the C-terminal domain
CC (755-1330) is essential for interaction with SPO11-1.
CC -!- DISRUPTION PHENOTYPE: Plants exhibit reduced fertility and meiotic
CC defects (PubMed:17762870). Drastic decrease in chiasma formation at
CC metaphase I associated with an absence of synapsis in prophase, due to
CC the inability to make double-strand breaks (DSB) (PubMed:19763177).
CC {ECO:0000269|PubMed:17762870, ECO:0000269|PubMed:19763177}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB46002.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78460.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; EF195233; ABQ12642.1; -; Genomic_DNA.
DR EMBL; Z97335; CAB46002.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161538; CAB78460.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83390.2; -; Genomic_DNA.
DR PIR; G85154; G85154.
DR RefSeq; NP_001319933.1; NM_001340905.1.
DR AlphaFoldDB; O23277; -.
DR SMR; O23277; -.
DR IntAct; O23277; 1.
DR MINT; O23277; -.
DR STRING; 3702.AT4G14180.1; -.
DR iPTMnet; O23277; -.
DR PaxDb; O23277; -.
DR PRIDE; O23277; -.
DR ProteomicsDB; 234838; -.
DR EnsemblPlants; AT4G14180.1; AT4G14180.1; AT4G14180.
DR GeneID; 827058; -.
DR Gramene; AT4G14180.1; AT4G14180.1; AT4G14180.
DR KEGG; ath:AT4G14180; -.
DR Araport; AT4G14180; -.
DR eggNOG; ENOG502QRT1; Eukaryota.
DR InParanoid; O23277; -.
DR OMA; SECKDPV; -.
DR OrthoDB; 86948at2759; -.
DR PhylomeDB; O23277; -.
DR PRO; PR:O23277; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O23277; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR044968; PRD1.
DR PANTHER; PTHR36379; PTHR36379; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Hydrolase; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..1330
FT /note="Protein PUTATIVE RECOMBINATION INITIATION DEFECT 1"
FT /id="PRO_0000346113"
FT REGION 1310..1330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 171
FT /note="N -> D (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 515
FT /note="V -> I (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 527..529
FT /note="KTI -> MTS (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 842
FT /note="I -> T (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 871
FT /note="I -> V (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 888
FT /note="R -> K (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 1025
FT /note="T -> A (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 1042
FT /note="H -> Y (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 1151
FT /note="M -> L (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
FT CONFLICT 1303
FT /note="F -> L (in Ref. 1; ABQ12642)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1330 AA; 149133 MW; 12EBB1AEDFE6C9BD CRC64;
MFFQHSQLQN SDHLLHESMA DSNHQSLSPP CANGHRSTIS LRDDQGGTFC LICFSNLVSD
PRIPTVHVSY ALHQLSIAIS EPIFLRTLLS SHIHFLVSPL VHALSSIDDA PIAIQIMDMI
SLLCSVEESS IGEDFVERIS DQLSSGALGW SRRQLHMLHC FGVLMSCENI NINSHIRDKE
ALVCQLVEGL QLPSEEIRGE ILFALYKFSA LQFTEQNVDG IEVLSLLCPK LLCLSLEALA
KTQRDDVRLN CVALLTILAQ QGLLANSHSN SASSMSLDEV DDDPMQTAEN VAARPCLNVL
FAEAIKGPLL STDSEVQIKT LDLIFHYISQ ESTPSKQIQV MVEENVADYI FEILRLSECK
DQVVNSCLRV LDLFSLAEHS FRKRLVIGFP SVIRVLHYVG EVPCHPFQIQ TLKLISSCIS
DFPGIASSSQ VQEIALVLKK MLERYYSQEM GLFPDAFAII CSVFVSLMKT PSFGETADVL
TSLQESLRHS ILASLSLPEK DSTQILHAVY LLNEVYVYCT ASTSINKTIC IELRHCVIDV
CTSHLLPWFL SDVNEVNEEA TLGIMETFHS ILLQNSDIQA KEFAELLVSA DWFSFSFGCL
GNFCTDNMKQ RIYLMLSSLV DILLEQKTGS HIRDALHCLP SDPQDLLFLL GQASSNNQEL
ASCQSAALLI FHTSSIYNDR LADDKLVLAS LEQYIILNKT SLICAISDSP ALLNLVNLYG
LCRSLQNERY QISYSLEAER IIFHLLNEYE WDLGSINIHL ESLKWLFQQE SISKSLIYQI
QKISRNNLIG NEVHNVYGDG RQRSLTYWFA KLISEGDNYA ATLLVNLLTQ LAEKEEQEND
VISILNLMNT IVSIFPTASN NLSMNGIGSV IHRLVSGFSN SSLGTSFRTL LLLVFNILTS
VQPAVLMIDE SWYAVSIKLL NFLSLRDTAI KQNHEDMVVI GILSLVLYHS SDGALVEASR
NIVSNSYLVS AINTVVDVAC SKGPALTQCQ DETNIGEALA FTLLLYFFSL RSLQIVLAGA
VDWQTFFGTS TSLETLPVVC IHCHNLCRLM HFGAPQIKLI ASYCLLELLT GLSEQVDIKK
EQLQCSSSYL KSMKAVLGGL VFCDDIRVAT NSALCLSMIL GWEDMEGRTE MLKTSSWYRF
IAEEMSVSLA MPCSASSTYV NHHKPAVYLT VAMLRLKNKP VWLRTVFDES CISSMIQNLN
GINISREIVI LFRELMQAEL LNSQQVTKLD RAFQECRKQM HRNGTRDETV EEQVQRKIPS
IHDHSEFCNY LVHLMVSNSF GHPSESETYT QKKKQILDEM EQFSELISTR EGRVSPIQEE
TRQMQTERIV
