PRD3_ARATH
ID PRD3_ARATH Reviewed; 449 AA.
AC Q0WWX5; A0A178WD31; A0A1P8AUM4; Q9LQ91;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2019, sequence version 2.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=Putative recombination initiation defects 3 {ECO:0000303|PubMed:19763177};
DE Short=AtPRD3 {ECO:0000303|PubMed:19763177};
GN Name=PRD3 {ECO:0000303|PubMed:19763177};
GN OrderedLocusNames=At1g01690 {ECO:0000312|Araport:AT1G01690};
GN ORFNames=T1N6.6 {ECO:0000312|EMBL:AAF78420.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-366.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19763177; DOI=10.1371/journal.pgen.1000654;
RA De Muyt A., Pereira L., Vezon D., Chelysheva L., Gendrot G., Chambon A.,
RA Laine-Choinard S., Pelletier G., Mercier R., Nogue F., Grelon M.;
RT "A high throughput genetic screen identifies new early meiotic
RT recombination functions in Arabidopsis thaliana.";
RL PLoS Genet. 5:E1000654-E1000654(2009).
RN [5]
RP INTERACTION WITH PRD1.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=28855712; DOI=10.1038/s41598-017-10270-9;
RA Tang Y., Yin Z., Zeng Y., Zhang Q., Chen L., He Y., Lu P., Ye D., Zhang X.;
RT "MTOPVIB interacts with AtPRD1 and plays important roles in formation of
RT meiotic DNA double-strand breaks in Arabidopsis.";
RL Sci. Rep. 7:10007-10007(2017).
CC -!- FUNCTION: Involved in DNA cleavage that forms the double-strand breaks
CC (DSB) that initiate meiotic recombination.
CC {ECO:0000269|PubMed:19763177}.
CC -!- SUBUNIT: Interacts with PRD1; this interaction facilitates a binding to
CC DFO. {ECO:0000269|PubMed:28855712}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Drastic decrease in chiasma formation at
CC metaphase I associated with an absence of synapsis in prophase, due to
CC the inability to make double-strand breaks (DSB).
CC {ECO:0000269|PubMed:19763177}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF78420.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=ANM60353.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC009273; AAF78420.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; ANM60353.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK226208; BAE98373.1; -; mRNA.
DR PIR; G86147; G86147.
DR RefSeq; NP_001322648.1; NM_001331292.1.
DR AlphaFoldDB; Q0WWX5; -.
DR STRING; 3702.AT1G01690.1; -.
DR GeneID; 839243; -.
DR KEGG; ath:AT1G01690; -.
DR Araport; AT1G01690; -.
DR PRO; PR:Q0WWX5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q0WWX5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0070192; P:chromosome organization involved in meiotic cell cycle; IEA:InterPro.
DR GO; GO:0048236; P:plant-type sporogenesis; IEA:InterPro.
DR InterPro; IPR034546; PAIR1.
DR PANTHER; PTHR37695; PTHR37695; 1.
PE 1: Evidence at protein level;
KW Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..449
FT /note="Putative recombination initiation defects 3"
FT /id="PRO_0000448441"
FT REGION 21..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 437..441
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 24..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 449 AA; 50737 MW; 877DD375E32A0869 CRC64;
MKMNINKACD LKSISVFPPN LRRSAEPQAS QQLRSQQSQQ SFSQGPSSSQ RGCGGFSQMT
QSSIDELLIN DQRFSSQERD LSLKKVSSCL PPINHKREDS QLVASRSSSG LSRRWSSASI
GESKSQISEE LEQRFGMMET SLSRFGMMLD SIQSDIMQAN RGTKEVFLET ERIQQKLTLQ
DTSLQQLRKE QADSKASLDG GVKFILEEFS KDPNQEKLQK ILQMLTTIPE QVETALQKIQ
REICHTFTRE IQVLASLRTP EPRVRVPTAP QVKAKENLPE QRGQAAKVLT SLKMPEPRVQ
VPAAPQAKEN FPEQRGPVAK SNSFCNTTLK TKQPQFPRNP NDASARAVKP YLSPKIQVGC
WKTVKPEKSN FKKRATRKPV KSESTRTQFE QCSVVIDSDE EDIDGGFSCL INENTRGTNF
EWDAEKETER ILRTARRTKR KFGNPIIIN
