AA7GT_DELGR
ID AA7GT_DELGR Reviewed; 505 AA.
AC E3W9M3;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 1.
DT 03-AUG-2022, entry version 40.
DE RecName: Full=Cyanidin 3-O-glucoside 7-O-glucosyltransferase (acyl-glucose);
DE Short=AA7GT;
DE Short=Dg AA7GT;
DE EC=2.4.1.300;
DE AltName: Full=Acyl-glucose-dependent anthocyanin 7-O-glucosytransferase;
DE AltName: Full=Beta-glucosidase like protein;
DE Short=DgBGLUL;
DE Flags: Precursor;
GN Name=AA7GT;
OS Delphinium grandiflorum (Siberian larkspur) (Delphinium sinense).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Ranunculoideae;
OC Delphinieae; Delphinium.
OX NCBI_TaxID=85439;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY,
RP BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, AND SUBCELLULAR LOCATION.
RC TISSUE=Petal;
RX PubMed=20971893; DOI=10.1105/tpc.110.077487;
RA Matsuba Y., Sasaki N., Tera M., Okamura M., Abe Y., Okamoto E.,
RA Nakamura H., Funabashi H., Takatsu M., Saito M., Matsuoka H., Nagasawa K.,
RA Ozeki Y.;
RT "A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose-
RT dependent glucosyltransferase in the petals of carnation and delphinium.";
RL Plant Cell 22:3374-3389(2010).
CC -!- FUNCTION: Beta-glycosidase that catalyzes the transfer of glucose
CC moiety to anthocyanidin 3-glucoside at the 7 position. Anthocyanins are
CC ubiquitous colored pigments that are responsible for variations in
CC petal color. {ECO:0000269|PubMed:20971893}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-O-(4-hydroxy-3-methoxybenzoyl)-beta-D-glucose + cyanidin 3-
CC O-beta-D-glucoside = cyanidin 3,7-di-O-beta-D-glucoside + vanillate;
CC Xref=Rhea:RHEA:35431, ChEBI:CHEBI:16632, ChEBI:CHEBI:71512,
CC ChEBI:CHEBI:71513, ChEBI:CHEBI:77857; EC=2.4.1.300;
CC Evidence={ECO:0000269|PubMed:20971893};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=22.9 mM for cyanidin 3-glucopyranoside (at pH 5.0 and 30 degrees
CC Celsius) {ECO:0000269|PubMed:20971893};
CC KM=260.8 mM for 1-O-beta-D-vanillyl-glucose (at pH 5.0 and 30 degrees
CC Celsius) {ECO:0000269|PubMed:20971893};
CC Note=kcat is 0.17 sec(-1) with cyanidin 3-glucopyranoside (at pH 5.0
CC and 30 degrees Celsius). kcat is 0.10 sec(-1) with 1-O-beta-D-
CC vanillyl-glucose (at pH 5.0 and 30 degrees Celsius).;
CC pH dependence:
CC Optimum pH is 6.0.;
CC Temperature dependence:
CC Optimum temperature is 40 degrees Celsius.
CC {ECO:0000269|PubMed:20971893};
CC -!- PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis.
CC {ECO:0000269|PubMed:20971893}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:20971893}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
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DR EMBL; AB510758; BAJ33502.1; -; mRNA.
DR AlphaFoldDB; E3W9M3; -.
DR SMR; E3W9M3; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR KEGG; ag:BAJ33502; -.
DR BioCyc; MetaCyc:MON-17864; -.
DR BRENDA; 2.4.1.300; 8950.
DR SABIO-RK; E3W9M3; -.
DR UniPathway; UPA00009; -.
DR GO; GO:0000325; C:plant-type vacuole; IDA:UniProtKB.
DR GO; GO:0102514; F:cyanidin 3-O-glucoside 7-O-glucosyltransferase (feruloyl-glucose dependent) activity; IEA:UniProtKB-EC.
DR GO; GO:0102507; F:cyanidin 3-O-glucoside 7-O-glucosyltransferase (hydroxybenzoly-glucose dependent) activity; IEA:UniProtKB-EC.
DR GO; GO:0102457; F:cyanidin 3-O-glucoside 7-O-glucosyltransferase (vanilloyl-glucose dependent) activity; IEA:UniProtKB-EC.
DR GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0102511; F:pelargonidin 3-O-glucoside 7-O-glucosyltransferase (acyl-glucose dependent) activity; IEA:UniProtKB-EC.
DR GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IDA:UniProtKB.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Glycosyltransferase; Signal; Transferase;
KW Vacuole.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..505
FT /note="Cyanidin 3-O-glucoside 7-O-glucosyltransferase
FT (acyl-glucose)"
FT /id="PRO_0000422569"
FT ACT_SITE 198
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 403
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 52
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 152
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 341
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 447
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 454..455
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 224
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 229
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 324
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 411
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 437
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 494
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 217..225
FT /evidence="ECO:0000250"
SQ SEQUENCE 505 AA; 57206 MW; 1B384D77B132BC9C CRC64;
MCPSFLVTLL LLQLSSLVVV LVVWAEQLPE FNVRRDDFPS NFVFGAGTSA LQVEGAIAED
GKTPNIWDVD SHMGHMPDKS TTDIACDSYH RYKEDVKIMS DIGLEAYRFS IAWTRILPYG
RGFINPKGVE YYNNLIDTLL EHGIQPHATI YHIDHPQILE DEYGGWLSPR MIEDFTTYAD
VCFREFGDRV SHWTTINEPN IISLGAYDSG QIPPHRCTPP GAYNCTAGNS SVEPYKAMHH
FLLAHASAVQ IYRTKYQAKQ KGLIGLNVYG FWCAPQTNSR ADIEATKRAT AFYTGWAADP
LVFGDYPIIM KENVGSRLPS FTKNESELVK GSFDFIGLNH YFVFYIQDDP EEITTPISLR
NFDSDMRVKA SVKPGDSGDP SGLKNLLRYF KDNYGNPPVY VHENGFGSPQ NETLDDDMGR
IRYISGYIGS MLEAIKNGSD TRGYFVWSFM DAFEILSGYQ TRYGIVHVDF DDKSLKRQLK
PSAQWYSNFI KKKNTTEDEI SYSSQ
