PRDX_CHAMQ
ID PRDX_CHAMQ Reviewed; 195 AA.
AC A0A2Z5VKM8; C0HLQ9;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2018, sequence version 1.
DT 25-MAY-2022, entry version 12.
DE RecName: Full=2-cysteine peroxiredoxin, chloroplastic {ECO:0000305};
DE Short=2-Cys Prx {ECO:0000305};
DE EC=1.11.1.24 {ECO:0000250|UniProtKB:Q96291};
DE AltName: Full=Thiol-specific antioxidant protein {ECO:0000305};
DE AltName: Full=Thioredoxin-dependent peroxiredoxin {ECO:0000305};
OS Chattonella marina var. antiqua (Red tide flagellate) (Chattonella
OS antiqua).
OG Plastid; Chloroplast {ECO:0000312|EMBL:BBB37582.1}.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Raphidophyceae; Chattonellales;
OC Chattonellaceae; Chattonella.
OX NCBI_TaxID=859642;
RN [1] {ECO:0000312|EMBL:BBB37582.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=NIES-1 {ECO:0000312|EMBL:BBB37582.1};
RA Mukai K., Teramoto A., Qiu X., Shimasaki Y., Kato-Unoki Y., Lee J.M.,
RA Mizoguchi N., Khanam M.R.M., Satone H., Tatsuke T., Kusakabe T., Oshima Y.;
RT "Gene structure and cDNA sequence of 2-Cys peroxiredoxin in the harmful
RT algal bloom species Chattonella marina and its gene transcription under
RT different light intensities.";
RL Eur. J. Phycol. 53:29-38(2018).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 2-21, AND INDUCTION.
RC STRAIN=NIES-1 {ECO:0000303|PubMed:23291769};
RX PubMed=23291769; DOI=10.1271/bbb.120543;
RA Qiu X., Shimasaki Y., Tsuyama M., Yamada T., Kuwahara R., Kawaguchi M.,
RA Honda M., Gunjikake H., Tasmin R., Shimizu M., Sato Y., Kato-Unoki Y.,
RA Nakashima T., Matsubara T., Yamasaki Y., Ichinose H., Wariishi H.,
RA Honjo T., Oshima Y.;
RT "Growth-phase dependent variation in photosynthetic activity and cellular
RT protein expression profile in the harmful raphidophyte Chattonella
RT antiqua.";
RL Biosci. Biotechnol. Biochem. 77:46-52(2013).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 2-21.
RC STRAIN=NIES-1 {ECO:0000303|Ref.3};
RX DOI=10.1016/j.jembe.2020.151361;
RA Qiu X., Mukai K., Shimasaki Y., Wu M., Chen C., Lu Y., Ichinose H.,
RA Nakashima T., Kato-Unoki Y., Oshima Y.;
RT "Diurnal variations in expression of photosynthesis-related proteins in the
RT harmful Raphidophyceae Chattonella marina var. antiqua.";
RL J. Exp. Mar. Biol. Ecol. 527:0-0(2020).
CC -!- FUNCTION: Thiol-specific peroxidase that catalyzes the reduction of
CC hydrogen peroxide and organic hydroperoxides to water and alcohols,
CC respectively. Plays a role in cell protection against oxidative stress
CC by detoxifying peroxides. {ECO:0000250|UniProtKB:Q96291}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-
CC disulfide + an alcohol + H2O; Xref=Rhea:RHEA:62620, Rhea:RHEA-
CC COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:29950, ChEBI:CHEBI:30879, ChEBI:CHEBI:35924,
CC ChEBI:CHEBI:50058; EC=1.11.1.24;
CC Evidence={ECO:0000250|UniProtKB:Q96291};
CC -!- SUBUNIT: Homodimer; disulfide-linked, upon oxidation.
CC {ECO:0000250|UniProtKB:Q06830}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000250|UniProtKB:Q96291}.
CC -!- INDUCTION: Up-regulated by high-intensity light and H2O2 (Ref.1). Down-
CC regulated in the late stationary growth phase as compared to the early
CC stationary and exponential growth phases (PubMed:23291769).
CC {ECO:0000269|PubMed:23291769, ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
CC {ECO:0000305}.
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DR EMBL; LC337661; BBB37582.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2Z5VKM8; -.
DR SMR; A0A2Z5VKM8; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0051920; F:peroxiredoxin activity; IEA:UniProtKB-EC.
DR InterPro; IPR000866; AhpC/TSA.
DR InterPro; IPR024706; Peroxiredoxin_AhpC-typ.
DR InterPro; IPR019479; Peroxiredoxin_C.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR InterPro; IPR013766; Thioredoxin_domain.
DR Pfam; PF10417; 1-cysPrx_C; 1.
DR Pfam; PF00578; AhpC-TSA; 1.
DR PIRSF; PIRSF000239; AHPC; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS51352; THIOREDOXIN_2; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Disulfide bond; Oxidoreductase;
KW Plastid.
FT CHAIN 1..195
FT /note="2-cysteine peroxiredoxin, chloroplastic"
FT /id="PRO_0000450269"
FT DOMAIN 3..161
FT /note="Thioredoxin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT ACT_SITE 49
FT /note="Cysteine sulfenic acid (-SOH) intermediate"
FT /evidence="ECO:0000305"
FT DISULFID 49
FT /note="Interchain (with C-173); in linked form"
FT /evidence="ECO:0000250|UniProtKB:Q06830"
FT DISULFID 170
FT /note="Interchain (with C-52); in linked form"
FT /evidence="ECO:0000250|UniProtKB:Q06830"
SQ SEQUENCE 195 AA; 22013 MW; CE6EA835BBFF5FDD CRC64;
MSIRVGQKAP DFTATAVFDQ EFGTIRLSDY LDKRYVVLFF YPLDFTFVCP TEITAFSDRF
KEFKELSTEV LGVSVDSEFS HLAWTQIDRK SGGLGELEYP LVSDLKKEIS SSYNVLTEDG
VALRALFIID KEGIIQHSTV NNLSFGRNVD EALRTLQAIQ YSQENPDEVC PVNWKPGSKT
MKPDPVGSKV YFEAI
