PRD_DROME
ID PRD_DROME Reviewed; 613 AA.
AC P06601; Q9VKB6;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 195.
DE RecName: Full=Segmentation protein paired;
GN Name=prd; ORFNames=CG6716;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B).
RX PubMed=2877746; DOI=10.1016/0092-8674(86)90516-7;
RA Frigerio G., Burri M., Bopp D., Baumgartner S., Noll M.;
RT "Structure of the segmentation gene paired and the Drosophila PRD gene set
RT as part of a gene network.";
RL Cell 47:735-746(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 27-154.
RX PubMed=7867071; DOI=10.1016/0092-8674(95)90518-9;
RA Xu W., Rould M.A., Jun S., Desplan C., Pabo C.O.;
RT "Crystal structure of a paired domain-DNA complex at 2.5-A resolution
RT reveals structural basis for Pax developmental mutations.";
RL Cell 80:639-650(1995).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 196-276.
RX PubMed=7671301; DOI=10.1016/0092-8674(95)90468-9;
RA Wilson D.S., Guenther B., Desplan C., Kuriyan J.;
RT "High resolution crystal structure of a paired (Pax) class cooperative
RT homeodomain dimer on DNA.";
RL Cell 82:709-719(1995).
CC -!- FUNCTION: Pair-rule protein expressed in a segmentally repeating
CC pattern to define the polarity of embryonic segments. Capable of
CC sequence-specific DNA-binding.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=P06601-1; Sequence=Displayed;
CC Name=A;
CC IsoId=P06601-2; Sequence=VSP_007021;
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M14548; AAB59221.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10801.1; -; Genomic_DNA.
DR EMBL; AE014134; AAF53160.1; -; Genomic_DNA.
DR EMBL; AY059447; AAL13353.1; -; mRNA.
DR PIR; A26062; A26062.
DR RefSeq; NP_523556.1; NM_078832.3. [P06601-2]
DR RefSeq; NP_723721.1; NM_164990.3. [P06601-1]
DR PDB; 1FJL; X-ray; 2.00 A; A/B/C=196-276.
DR PDB; 1PDN; X-ray; 2.50 A; C=27-154.
DR PDBsum; 1FJL; -.
DR PDBsum; 1PDN; -.
DR AlphaFoldDB; P06601; -.
DR SMR; P06601; -.
DR BioGRID; 60680; 17.
DR DIP; DIP-22888N; -.
DR IntAct; P06601; 3.
DR STRING; 7227.FBpp0079882; -.
DR PaxDb; P06601; -.
DR DNASU; 34629; -.
DR EnsemblMetazoa; FBtr0080298; FBpp0079882; FBgn0003145. [P06601-1]
DR EnsemblMetazoa; FBtr0080299; FBpp0079883; FBgn0003145. [P06601-2]
DR GeneID; 34629; -.
DR KEGG; dme:Dmel_CG6716; -.
DR UCSC; CG6716-RA; d. melanogaster. [P06601-1]
DR CTD; 5548; -.
DR FlyBase; FBgn0003145; prd.
DR VEuPathDB; VectorBase:FBgn0003145; -.
DR eggNOG; KOG0849; Eukaryota.
DR GeneTree; ENSGT00940000168362; -.
DR HOGENOM; CLU_028364_0_0_1; -.
DR InParanoid; P06601; -.
DR OMA; PHAHPQY; -.
DR PhylomeDB; P06601; -.
DR Reactome; R-DME-3214847; HATs acetylate histones.
DR SignaLink; P06601; -.
DR BioGRID-ORCS; 34629; 0 hits in 3 CRISPR screens.
DR EvolutionaryTrace; P06601; -.
DR GenomeRNAi; 34629; -.
DR PRO; PR:P06601; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0003145; Expressed in embryonic foregut sensory structure (Drosophila) and 29 other tissues.
DR Genevisible; P06601; DM.
DR GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0007366; P:periodic partitioning by pair rule gene; TAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR CDD; cd00131; PAX; 1.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR043182; PAIRED_DNA-bd_dom.
DR InterPro; IPR001523; Paired_dom.
DR InterPro; IPR043565; PAX_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45636; PTHR45636; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00292; PAX; 1.
DR PRINTS; PR00027; PAIREDBOX.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00351; PAX; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00034; PAIRED_1; 1.
DR PROSITE; PS51057; PAIRED_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Developmental protein; DNA-binding;
KW Homeobox; Nucleus; Pair-rule protein; Paired box; Reference proteome;
KW Repeat; Transcription; Transcription regulation.
FT CHAIN 1..613
FT /note="Segmentation protein paired"
FT /id="PRO_0000050167"
FT DNA_BIND 27..153
FT /note="Paired"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT DNA_BIND 213..272
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 30..86
FT /note="PAI subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 105..153
FT /note="RED subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 154..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 403..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 534..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 160..188
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..23
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_007021"
FT VARIANT 164
FT /note="A -> T"
FT VARIANT 220
FT /note="F -> I"
FT STRAND 29..31
FT /evidence="ECO:0007829|PDB:1PDN"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:1PDN"
FT HELIX 46..57
FT /evidence="ECO:0007829|PDB:1PDN"
FT HELIX 62..69
FT /evidence="ECO:0007829|PDB:1PDN"
FT HELIX 73..86
FT /evidence="ECO:0007829|PDB:1PDN"
FT HELIX 106..112
FT /evidence="ECO:0007829|PDB:1PDN"
FT TURN 113..116
FT /evidence="ECO:0007829|PDB:1PDN"
FT HELIX 122..131
FT /evidence="ECO:0007829|PDB:1PDN"
FT STRAND 133..135
FT /evidence="ECO:0007829|PDB:1PDN"
FT HELIX 143..149
FT /evidence="ECO:0007829|PDB:1PDN"
FT HELIX 222..234
FT /evidence="ECO:0007829|PDB:1FJL"
FT HELIX 240..250
FT /evidence="ECO:0007829|PDB:1FJL"
FT HELIX 254..275
FT /evidence="ECO:0007829|PDB:1FJL"
SQ SEQUENCE 613 AA; 65497 MW; 777772C6E704C1A2 CRC64;
MTVTAFAAAM HRPFFNGYST MQDMNSGQGR VNQLGGVFIN GRPLPNNIRL KIVEMAADGI
RPCVISRQLR VSHGCVSKIL NRYQETGSIR PGVIGGSKPR IATPEIENRI EEYKRSSPGM
FSWEIREKLI REGVCDRSTA PSVSAISRLV RGRDAPLDND MSSASGSPAG DGTKASSSCG
SDVSGGHHNN GKPSDEDISD CESEPGIALK RKQRRCRTTF SASQLDELER AFERTQYPDI
YTREELAQRT NLTEARIQVW FSNRRARLRK QHTSVSGGAP GGAAASVSHV AASSSLPSVV
SSVPSMAPLA MMPGSLDPAT VYQQQYDFYG SHANISVSAA APMASSNLSP GITTTPPHHH
QFYNPSANTA SYIMPGENGN TTPTGNIIVS SYETQLGSVY GTETETHQTM PRNESPNESV
SSAFGQLPPT PNSLSAVVSG AGVTSSSGAN SGADPSQSLA NASAGSEELS AALKVESVDL
IAASQSQLYG GWSSMQALRP NAPLSPEDSL NSTSSTSQAL DVTAHQMFHP YQHTPQYASY
PAPGHAHSHH GHPHAPHPHA HPHPQYAGAH PHYPPPSSSA HFMPQNFNAA AFPSPSKVNY
TTMPPQPFYP SWY
