ID   PRE1_STAAU              Reviewed;         403 AA.
AC   P03857;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Plasmid recombination enzyme type 1;
DE   AltName: Full=Mobilization protein;
DE   AltName: Full=Plasmid recombinase;
GN   Name=pre; Synonyms=mob;
OS   Staphylococcus aureus.
OG   Plasmid pE194.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6279574; DOI=10.1128/jb.150.2.804-814.1982;
RA   Horinouchi S., Weisblum B.;
RT   "Nucleotide sequence and functional map of pE194, a plasmid that specifies
RT   inducible resistance to macrolide, lincosamide, and streptogramin type B
RT   antibodies.";
RL   J. Bacteriol. 150:804-814(1982).
CC   -!- FUNCTION: The interaction of the RSA site and the PRE protein may not
CC       only serves a function in plasmid maintenance, but may also contributes
CC       to the distribution of small antibiotic resistance plasmids among Gram-
CC       positive bacteria.
CC   -!- MISCELLANEOUS: Contains conserved positively charged amino acids
CC       probably involved in the binding of the pre protein to the RSA site.
CC   -!- SIMILARITY: Belongs to the plasmid mobilization pre family.
CC       {ECO:0000305}.
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DR   EMBL; V01278; CAA24593.1; -; Genomic_DNA.
DR   PIR; A04486; QQSA4E.
DR   RefSeq; WP_011178348.1; NC_005908.1.
DR   RefSeq; YP_025317.1; NC_005908.1.
DR   AlphaFoldDB; P03857; -.
DR   SMR; P03857; -.
DR   PRIDE; P03857; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   InterPro; IPR001668; Mob_Pre.
DR   Pfam; PF01076; Mob_Pre; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Plasmid.
FT   CHAIN           1..403
FT                   /note="Plasmid recombination enzyme type 1"
FT                   /id="PRO_0000068420"
FT   REGION          10..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..26
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         45
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
FT   BINDING         115
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   403 AA;  47839 MW;  875867A394000FC1 CRC64;
     MSHSILRVAR VKGSSNTNGI QRHNQRENKN YNNKDINHEE TYKNYDLINA QNIKYKDKID
     ETIDENYSGK RKIRSDAIRH VDGLVTSDKD FFDDLSGEEI ERFFKDSLEF LENEYGKENM
     LYATVHLDER VPHMHFGFVP LTEDGRLSAK EQLGNKKDFT QLQDRFNEYV NEKGYELERG
     TSKEVTEREH KAMDQYKKDT VFHKQELQEV KDELQKANKQ LQSGIEHMRS TKPFDYENER
     TGLFSGREET GRKILTADEF ERLQETISSA ERIVDDYENI KSTDYYTENQ ELKKRRESLK
     EVVNTWKEGY HEKSKEVNKL KRENDSLNEQ LNVSEKFQAS TVTLYRAARA NFPGFEKGFN
     RLKEKFFNDS KFERVGQFMD VVQDNVQKVD RKREKQRTDD LEM