ID   PRE2_ARATH              Reviewed;          94 AA.
AC   Q9LXG5;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Transcription factor PRE2;
DE   AltName: Full=Basic helix-loop-helix protein 134;
DE            Short=AtbHLH134;
DE            Short=bHLH 134;
DE   AltName: Full=Protein BANQUO 2;
DE   AltName: Full=Protein PACLOBUTRAZOL RESISTANCE 2;
DE   AltName: Full=Transcription factor EN 52;
DE   AltName: Full=bHLH transcription factor bHLH134;
GN   Name=PRE2; Synonyms=BHLH134, BNQ2, EN52; OrderedLocusNames=At5g15160;
GN   ORFNames=F8M21.50;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11910074; DOI=10.1126/science.1071006;
RA   Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA   Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA   Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA   Shinagawa A., Shinozaki K.;
RT   "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL   Science 296:141-145(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=12897250; DOI=10.1105/tpc.013839;
RA   Toledo-Ortiz G., Huq E., Quail P.H.;
RT   "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL   Plant Cell 15:1749-1770(2003).
RN   [6]
RP   FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=16527868; DOI=10.1093/pcp/pcj026;
RA   Lee S., Lee S., Yang K.Y., Kim Y.M., Park S.Y., Kim S.Y., Soh M.S.;
RT   "Overexpression of PRE1 and its homologous genes activates gibberellin-
RT   dependent responses in Arabidopsis thaliana.";
RL   Plant Cell Physiol. 47:591-600(2006).
RN   [7]
RP   FUNCTION, AND INTERACTION WITH HFR1.
RX   PubMed=20305124; DOI=10.1105/tpc.109.065946;
RA   Mara C.D., Huang T., Irish V.F.;
RT   "The Arabidopsis floral homeotic proteins APETALA3 and PISTILLATA
RT   negatively regulate the BANQUO genes implicated in light signaling.";
RL   Plant Cell 22:690-702(2010).
CC   -!- FUNCTION: Atypical and probable non DNA-binding bHLH transcription
CC       factor that integrates multiple signaling pathways to regulate cell
CC       elongation and plant development. Regulates light responses by binding
CC       and inhibiting the activity of the bHLH transcription factor HFR1, a
CC       critical regulator of light signaling and shade avoidance. May have a
CC       regulatory role in various aspects of gibberellin-dependent growth and
CC       development. {ECO:0000269|PubMed:16527868,
CC       ECO:0000269|PubMed:20305124}.
CC   -!- SUBUNIT: Interacts with HFR1. {ECO:0000269|PubMed:20305124}.
CC   -!- INTERACTION:
CC       Q9LXG5; Q9C8Z9: BHLH148; NbExp=5; IntAct=EBI-4459591, EBI-4434374;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and flowers.
CC       {ECO:0000269|PubMed:16527868}.
CC   -!- INDUCTION: Not induced by exogenous gibberellin.
CC       {ECO:0000269|PubMed:16527868}.
CC   -!- MISCELLANEOUS: Plants over-expressing PRE2 show long hypocotyls, pale
CC       green and slightly narrow leaves, elongated petioles and early
CC       flowering. They are not sensitive to the gibberellin inhibitor
CC       paclobutrazol during seed germination (PubMed:16527868).
CC       {ECO:0000305|PubMed:16527868}.
CC   -!- SIMILARITY: Belongs to the bHLH protein family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL353993; CAB89326.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED92123.1; -; Genomic_DNA.
DR   EMBL; AK118887; BAC43472.1; -; mRNA.
DR   EMBL; BT005460; AAO63880.1; -; mRNA.
DR   PIR; T49951; T49951.
DR   RefSeq; NP_197020.1; NM_121520.4.
DR   AlphaFoldDB; Q9LXG5; -.
DR   SMR; Q9LXG5; -.
DR   BioGRID; 16645; 6.
DR   IntAct; Q9LXG5; 6.
DR   STRING; 3702.AT5G15160.1; -.
DR   iPTMnet; Q9LXG5; -.
DR   PaxDb; Q9LXG5; -.
DR   PRIDE; Q9LXG5; -.
DR   ProteomicsDB; 234870; -.
DR   EnsemblPlants; AT5G15160.1; AT5G15160.1; AT5G15160.
DR   GeneID; 831368; -.
DR   Gramene; AT5G15160.1; AT5G15160.1; AT5G15160.
DR   KEGG; ath:AT5G15160; -.
DR   Araport; AT5G15160; -.
DR   TAIR; locus:2150916; AT5G15160.
DR   eggNOG; ENOG502S648; Eukaryota.
DR   HOGENOM; CLU_183267_0_0_1; -.
DR   InParanoid; Q9LXG5; -.
DR   OMA; IGYISTH; -.
DR   OrthoDB; 1589357at2759; -.
DR   PhylomeDB; Q9LXG5; -.
DR   PRO; PR:Q9LXG5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LXG5; baseline and differential.
DR   Genevisible; Q9LXG5; AT.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0009640; P:photomorphogenesis; IMP:TAIR.
DR   GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR   GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR044293; PRE.
DR   PANTHER; PTHR46446; PTHR46446; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   1: Evidence at protein level;
KW   Gibberellin signaling pathway; Growth regulation; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..94
FT                   /note="Transcription factor PRE2"
FT                   /id="PRO_0000429084"
FT   DOMAIN          5..60
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ   SEQUENCE   94 AA;  10546 MW;  DCE5E5CF011B4EC3 CRC64;
     MSSSRRSRQA SSSSRISDDQ ITDLISKLRQ SIPEIRQNRR SNTVSASKVL QETCNYIRNL
     NKEADDLSDR LTQLLESIDP NSPQAAVIRS LING