PRE3_ARATH
ID PRE3_ARATH Reviewed; 93 AA.
AC Q9CA64;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Transcription factor PRE3;
DE AltName: Full=Basic helix-loop-helix protein 135;
DE Short=AtbHLH135;
DE Short=bHLH 135;
DE AltName: Full=Protein ACTIVATION-TAGGED BRI1 SUPPRESSOR 1;
DE Short=ATBS1;
DE AltName: Full=Protein PACLOBUTRAZOL RESISTANCE 3;
DE AltName: Full=Protein TARGET OF MOOPTEROS 7;
DE AltName: Full=Transcription factor EN 67;
DE AltName: Full=bHLH transcription factor bHLH135;
GN Name=PRE3; Synonyms=BHLH135, BS1, EN67, TMO7; OrderedLocusNames=At1g74500;
GN ORFNames=F1M20.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=16527868; DOI=10.1093/pcp/pcj026;
RA Lee S., Lee S., Yang K.Y., Kim Y.M., Park S.Y., Kim S.Y., Soh M.S.;
RT "Overexpression of PRE1 and its homologous genes activates gibberellin-
RT dependent responses in Arabidopsis thaliana.";
RL Plant Cell Physiol. 47:591-600(2006).
RN [9]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND INTERACTION WITH
RP BHLH147; BHLH148; BHLH149 AND BHLH150.
RC STRAIN=cv. Columbia;
RX PubMed=20023194; DOI=10.1105/tpc.109.072504;
RA Wang H., Zhu Y., Fujioka S., Asami T., Li J., Li J.;
RT "Regulation of Arabidopsis brassinosteroid signaling by atypical basic
RT helix-loop-helix proteins.";
RL Plant Cell 21:3781-3791(2009).
RN [10]
RP FUNCTION, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=20220754; DOI=10.1038/nature08836;
RA Schlereth A., Moller B., Liu W., Kientz M., Flipse J., Rademacher E.H.,
RA Schmid M., Jurgens G., Weijers D.;
RT "MONOPTEROS controls embryonic root initiation by regulating a mobile
RT transcription factor.";
RL Nature 464:913-916(2010).
RN [11]
RP INTERACTION WITH SIEL.
RX PubMed=21924907; DOI=10.1016/j.cub.2011.08.013;
RA Koizumi K., Wu S., MacRae-Crerar A., Gallagher K.L.;
RT "An essential protein that interacts with endosomes and promotes movement
RT of the SHORT-ROOT transcription factor.";
RL Curr. Biol. 21:1559-1564(2011).
RN [12]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=22339648; DOI=10.1111/j.1399-3054.2012.01600.x;
RA Castelain M., Le Hir R., Bellini C.;
RT "The non-DNA-binding bHLH transcription factor PRE3/bHLH135/ATBS1/TMO7 is
RT involved in the regulation of light signaling pathway in Arabidopsis.";
RL Physiol. Plantarum 145:450-460(2012).
RN [13]
RP INTERACTION WITH IBH1.
RX PubMed=23161888; DOI=10.1105/tpc.112.105023;
RA Ikeda M., Fujiwara S., Mitsuda N., Ohme-Takagi M.;
RT "A triantagonistic basic helix-loop-helix system regulates cell elongation
RT in Arabidopsis.";
RL Plant Cell 24:4483-4497(2012).
CC -!- FUNCTION: Atypical and probable non DNA-binding bHLH transcription
CC factor required for MONOPTEROS-dependent root initiation in embryo.
CC Promotes the correct definition of the hypophysis cell division plane.
CC Transcriptionally controlled by MONOPTEROS. Moves from its site of
CC synthesis in pro-embryos cells into the hypophysis. Regulates
CC brassinosteroid (BR) signaling by sequestering negative BR signaling
CC components. May function as positive regulator of gibberellin
CC signaling. May play a role in the regulation of light signaling and
CC possibly auxin signaling. {ECO:0000269|PubMed:16527868,
CC ECO:0000269|PubMed:20023194, ECO:0000269|PubMed:20220754,
CC ECO:0000269|PubMed:22339648}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with BHLH 147, BHLH148,
CC BHLH149, BHLH150 and IBH1. Interacts with SIEL (PubMed:21924907).
CC {ECO:0000269|PubMed:20023194, ECO:0000269|PubMed:21924907,
CC ECO:0000269|PubMed:23161888, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20023194,
CC ECO:0000269|PubMed:20220754}. Cytoplasm {ECO:0000269|PubMed:20220754}.
CC -!- TISSUE SPECIFICITY: Expressed in root and shoot meristems, and young
CC siliques. Low levels detected in all aerial tissues.
CC {ECO:0000269|PubMed:16527868, ECO:0000269|PubMed:20023194,
CC ECO:0000269|PubMed:22339648}.
CC -!- DEVELOPMENTAL STAGE: At the globular stage, expressed in cells adjacent
CC to the hypophysis and at later embryonic stages, restricted to the
CC future root stem cells. {ECO:0000269|PubMed:20220754}.
CC -!- INDUCTION: Not induced by exogenous gibberellin.
CC {ECO:0000269|PubMed:16527868}.
CC -!- MISCELLANEOUS: Plants over-expressing PRE3 show long hypocotyls, pale
CC green and slightly narrow leaves, elongated petioles and early
CC flowering. They are not sensitive to the gibberellin inhibitor
CC paclobutrazol during seed germination (PubMed:16527868,
CC PubMed:22339648). {ECO:0000305|PubMed:16527868,
CC ECO:0000305|PubMed:22339648}.
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DR EMBL; AC011765; AAG52350.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35600.1; -; Genomic_DNA.
DR EMBL; BT003046; AAO23611.1; -; mRNA.
DR EMBL; AK227395; BAE99399.1; -; mRNA.
DR EMBL; AY088286; AAM65825.1; -; mRNA.
DR PIR; A96774; A96774.
DR RefSeq; NP_177590.1; NM_106110.3.
DR AlphaFoldDB; Q9CA64; -.
DR SMR; Q9CA64; -.
DR BioGRID; 29010; 5.
DR IntAct; Q9CA64; 4.
DR STRING; 3702.AT1G74500.1; -.
DR PaxDb; Q9CA64; -.
DR PRIDE; Q9CA64; -.
DR ProteomicsDB; 236598; -.
DR EnsemblPlants; AT1G74500.1; AT1G74500.1; AT1G74500.
DR GeneID; 843791; -.
DR Gramene; AT1G74500.1; AT1G74500.1; AT1G74500.
DR KEGG; ath:AT1G74500; -.
DR Araport; AT1G74500; -.
DR TAIR; locus:2019210; AT1G74500.
DR eggNOG; ENOG502S4KP; Eukaryota.
DR HOGENOM; CLU_183267_0_0_1; -.
DR InParanoid; Q9CA64; -.
DR OMA; APSMITD; -.
DR OrthoDB; 1589357at2759; -.
DR PhylomeDB; Q9CA64; -.
DR PRO; PR:Q9CA64; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CA64; baseline and differential.
DR Genevisible; Q9CA64; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IGI:TAIR.
DR GO; GO:0010086; P:embryonic root morphogenesis; IMP:TAIR.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0048364; P:root development; IEP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR044293; PRE.
DR PANTHER; PTHR46446; PTHR46446; 1.
DR Pfam; PF00010; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Brassinosteroid signaling pathway; Cytoplasm;
KW Gibberellin signaling pathway; Growth regulation; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..93
FT /note="Transcription factor PRE3"
FT /id="PRO_0000358817"
FT DOMAIN 6..61
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ SEQUENCE 93 AA; 10581 MW; D46F5F38304E483E CRC64;
MSGRRSRSRQ SSGTSRISED QINDLIIKLQ QLLPELRDSR RSDKVSAARV LQDTCNYIRN
LHREVDDLSE RLSELLANSD TAQAALIRSL LTQ
