ID   PRE3_MAMLE              Reviewed;         413 AA.
AC   Q5DU99;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Plasmid recombination enzyme type 3;
DE   AltName: Full=Mobilization protein;
DE   AltName: Full=Plasmid recombinase;
GN   Name=pre;
OS   Mammaliicoccus lentus (Staphylococcus lentus).
OG   Plasmid pSTE2.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Mammaliicoccus.
OX   NCBI_TaxID=42858;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=44;
RX   PubMed=15980097; DOI=10.1093/jac/dki207;
RA   Hauschild T., Luehtje P., Schwarz S.;
RT   "Staphylococcal tetracycline-MLSB resistance plasmid pSTE2 is the product
RT   of an RSA-mediated in vivo recombination.";
RL   J. Antimicrob. Chemother. 56:399-402(2005).
CC   -!- FUNCTION: The interaction of the RSA site and the PRE protein may not
CC       only serves a function in plasmid maintenance, but may also contributes
CC       to the distribution of small antibiotic resistance plasmids among Gram-
CC       positive bacteria. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Contains conserved positively charged amino acids
CC       probably involved in the binding of the pre protein to the RSA site.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plasmid mobilization pre family.
CC       {ECO:0000305}.
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DR   EMBL; AJ888003; CAI59790.1; -; Genomic_DNA.
DR   RefSeq; WP_000122374.1; NC_006871.1.
DR   RefSeq; YP_209655.1; NC_006871.1.
DR   AlphaFoldDB; Q5DU99; -.
DR   SMR; Q5DU99; -.
DR   GeneID; 50019750; -.
DR   GeneID; 61682014; -.
DR   GeneID; 66909090; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   InterPro; IPR001668; Mob_Pre.
DR   Pfam; PF01076; Mob_Pre; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Plasmid.
FT   CHAIN           1..413
FT                   /note="Plasmid recombination enzyme type 3"
FT                   /id="PRO_0000224929"
FT   REGION          179..218
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          374..413
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        179..193
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        194..218
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         45
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
FT   BINDING         115
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   413 AA;  48526 MW;  8F16FF8D82998659 CRC64;
     MSYSIVRVSK VKSGTNTTGI QKHVQRENNN YENEDIDHSK TYLNYDLVNA NKQNFNNLID
     EKIEQNYTGK RKIRTDAIKH IDGLITSDND FFDNQTPEDT KQFFEYAKEF LEQEYGKDNL
     LYATVHMDEK TPHMHYGVVP ITDDGRLSAK EVVGNKKALT AFQDRFNEHV KQRGYDLERG
     QSRQVTNAKH EQISQYKQKT EYHKQEYERE SQKTDHIKQK NDKLMQEYQK SLNTLKKPIN
     VPYEQETEKV GGLFSKEIQE TGNVVISQKD FNEFQKQIKA AQDISEDYEY IKSGRALDDK
     DKEIREKDDL LNKAVERIEN ADDNFNQLYE NAKPLKENIE IALKLLKILL KELERVLGRN
     TFAERVNKLT EDEPKLNGLA GNLDKKMNPE LYSEQEQQQE QQKNQKRDRG MHL