ID   PRE3_STAAC              Reviewed;         413 AA.
AC   Q5HJZ6;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Plasmid recombination enzyme type 3;
DE   AltName: Full=Mobilization protein;
DE   AltName: Full=Plasmid recombinase;
GN   Name=pre; OrderedLocusNames=SAA0003;
OS   Staphylococcus aureus (strain COL).
OG   Plasmid pT181.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: The interaction of the RSA site and the PRE protein may not
CC       only serves a function in plasmid maintenance, but may also contributes
CC       to the distribution of small antibiotic resistance plasmids among Gram-
CC       positive bacteria. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Contains conserved positively charged amino acids
CC       probably involved in the binding of the pre protein to the RSA site.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plasmid mobilization pre family.
CC       {ECO:0000305}.
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DR   EMBL; CP000045; AAW36314.1; -; Genomic_DNA.
DR   RefSeq; WP_000122374.1; NC_006629.2.
DR   AlphaFoldDB; Q5HJZ6; -.
DR   SMR; Q5HJZ6; -.
DR   EnsemblBacteria; AAW36314; AAW36314; SAA0003.
DR   GeneID; 50019750; -.
DR   GeneID; 61682014; -.
DR   GeneID; 66909090; -.
DR   KEGG; sac:SAA0003; -.
DR   HOGENOM; CLU_035698_0_0_9; -.
DR   OMA; VEDYFER; -.
DR   Proteomes; UP000000530; Plasmid pT181.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   InterPro; IPR001668; Mob_Pre.
DR   Pfam; PF01076; Mob_Pre; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Plasmid.
FT   CHAIN           1..413
FT                   /note="Plasmid recombination enzyme type 3"
FT                   /id="PRO_0000224927"
FT   REGION          179..218
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          374..413
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        179..193
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        194..218
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         45
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
FT   BINDING         115
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   413 AA;  48526 MW;  8F16FF8D82998659 CRC64;
     MSYSIVRVSK VKSGTNTTGI QKHVQRENNN YENEDIDHSK TYLNYDLVNA NKQNFNNLID
     EKIEQNYTGK RKIRTDAIKH IDGLITSDND FFDNQTPEDT KQFFEYAKEF LEQEYGKDNL
     LYATVHMDEK TPHMHYGVVP ITDDGRLSAK EVVGNKKALT AFQDRFNEHV KQRGYDLERG
     QSRQVTNAKH EQISQYKQKT EYHKQEYERE SQKTDHIKQK NDKLMQEYQK SLNTLKKPIN
     VPYEQETEKV GGLFSKEIQE TGNVVISQKD FNEFQKQIKA AQDISEDYEY IKSGRALDDK
     DKEIREKDDL LNKAVERIEN ADDNFNQLYE NAKPLKENIE IALKLLKILL KELERVLGRN
     TFAERVNKLT EDEPKLNGLA GNLDKKMNPE LYSEQEQQQE QQKNQKRDRG MHL