ID   PRE3_STAAU              Reviewed;         413 AA.
AC   P03864;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Plasmid recombination enzyme type 3;
DE   AltName: Full=Mobilization protein;
DE   AltName: Full=Plasmid recombinase;
GN   Name=pre;
OS   Staphylococcus aureus.
OG   Plasmid pT181.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3584064; DOI=10.1128/jb.169.6.2601-2610.1987;
RA   Gennaro M.L., Kornblum J., Novick R.P.;
RT   "A site-specific recombination function in Staphylococcus aureus
RT   plasmids.";
RL   J. Bacteriol. 169:2601-2610(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 225-413.
RX   PubMed=6657777; DOI=10.1016/0147-619x(83)90039-2;
RA   Khan S.A., Novick R.P.;
RT   "Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid
RT   from Staphylococcus aureus.";
RL   Plasmid 10:251-259(1983).
CC   -!- FUNCTION: The interaction of the RSA site and the PRE protein may not
CC       only serves a function in plasmid maintenance, but may also contributes
CC       to the distribution of small antibiotic resistance plasmids among Gram-
CC       positive bacteria.
CC   -!- MISCELLANEOUS: Contains conserved positively charged amino acids
CC       probably involved in the binding of the pre protein to the RSA site.
CC   -!- SIMILARITY: Belongs to the plasmid mobilization pre family.
CC       {ECO:0000305}.
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DR   EMBL; J01764; AAA26036.1; -; Genomic_DNA.
DR   PIR; A04493; QQSA8T.
DR   RefSeq; NP_040472.1; NC_001393.1.
DR   RefSeq; WP_010889940.1; NC_001393.1.
DR   AlphaFoldDB; P03864; -.
DR   SMR; P03864; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   InterPro; IPR001668; Mob_Pre.
DR   Pfam; PF01076; Mob_Pre; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Plasmid.
FT   CHAIN           1..413
FT                   /note="Plasmid recombination enzyme type 3"
FT                   /id="PRO_0000068424"
FT   REGION          175..198
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          374..413
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        179..193
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         45
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
FT   BINDING         115
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        364
FT                   /note="E -> G (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        367
FT                   /note="N -> S (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   413 AA;  48438 MW;  BFC9181A1CF4D4EC CRC64;
     MSYSIVRVSK VKSGTNTTGI QKHVQRENNN YENEDIDHSK TYLNYDLVNA NKQNFNNLID
     EKIEQNYTGK RKIRTDAIKH IDGLITSDND FFDNQTPEDT KQFFEYAKEF LEQEYGKDNL
     LYATVHMDEK TPHMHYGVVP ITDDGRLSAK EVVGNKKALT AFQDRFNEHV KQRGYGLERG
     QSRQVTNAKH EQISQYKQKT EYHKQEYERE SQKTDHIKQK NDKLMQEYQK SLNTLKKPIN
     VPYEQETEKV GGLFSKEIQE AGNVVISQKD FNEFQKQIKA AQDISEDYEY IKSGRALDDK
     DKEIREKDDL LNKAVERIEN ADDNFNQLYE NAKPLKENIE IALKLLKILL KELERVLGRN
     TFAERVNKLT EDEPKLNGLA GNLDKKMNPE LYSEQEQQQE QQKNQKRDRG MHL