ID   PRE4_ARATH              Reviewed;          92 AA.
AC   F4JCN9; Q9STU2;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Transcription factor PRE4;
DE   AltName: Full=Basic helix-loop-helix protein 161;
DE            Short=AtbHLH161;
DE            Short=bHLH 161;
DE   AltName: Full=Protein BANQUO 3;
DE   AltName: Full=Protein PACLOBUTRAZOL RESISTANCE 4;
DE   AltName: Full=bHLH transcription factor bHLH161;
GN   Name=PRE4; Synonyms=BHLH161, BNQ3; OrderedLocusNames=At3g47710;
GN   ORFNames=T23J7.40;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=14600211; DOI=10.1105/tpc.151140;
RA   Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA   Jakoby M., Werber M., Weisshaar B.;
RT   "Update on the basic helix-loop-helix transcription factor gene family in
RT   Arabidopsis thaliana.";
RL   Plant Cell 15:2497-2502(2003).
RN   [4]
RP   FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=16527868; DOI=10.1093/pcp/pcj026;
RA   Lee S., Lee S., Yang K.Y., Kim Y.M., Park S.Y., Kim S.Y., Soh M.S.;
RT   "Overexpression of PRE1 and its homologous genes activates gibberellin-
RT   dependent responses in Arabidopsis thaliana.";
RL   Plant Cell Physiol. 47:591-600(2006).
RN   [5]
RP   FUNCTION, INTERACTION WITH HFR1, AND DISRUPTION PHENOTYPE.
RX   PubMed=20305124; DOI=10.1105/tpc.109.065946;
RA   Mara C.D., Huang T., Irish V.F.;
RT   "The Arabidopsis floral homeotic proteins APETALA3 and PISTILLATA
RT   negatively regulate the BANQUO genes implicated in light signaling.";
RL   Plant Cell 22:690-702(2010).
RN   [6]
RP   INTERACTION WITH IBH1.
RX   PubMed=23161888; DOI=10.1105/tpc.112.105023;
RA   Ikeda M., Fujiwara S., Mitsuda N., Ohme-Takagi M.;
RT   "A triantagonistic basic helix-loop-helix system regulates cell elongation
RT   in Arabidopsis.";
RL   Plant Cell 24:4483-4497(2012).
CC   -!- FUNCTION: Atypical and probable non DNA-binding bHLH transcription
CC       factor that integrates multiple signaling pathways to regulate cell
CC       elongation and plant development. Regulates light responses by binding
CC       and inhibiting the activity of the bHLH transcription factor HFR1, a
CC       critical regulator of light signaling and shade avoidance. May have a
CC       regulatory role in various aspects of gibberellin-dependent growth and
CC       development. {ECO:0000269|PubMed:16527868,
CC       ECO:0000269|PubMed:20305124}.
CC   -!- SUBUNIT: Interacts with HFR1 and IBH1. {ECO:0000269|PubMed:20305124,
CC       ECO:0000269|PubMed:23161888}.
CC   -!- INTERACTION:
CC       F4JCN9; O80482: BHLH149; NbExp=3; IntAct=EBI-15192623, EBI-15193669;
CC       F4JCN9; Q0V7X4: FIT; NbExp=4; IntAct=EBI-15192623, EBI-1640543;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and flowers.
CC       {ECO:0000269|PubMed:16527868}.
CC   -!- INDUCTION: Not induced by exogenous gibberellin.
CC       {ECO:0000269|PubMed:16527868}.
CC   -!- DISRUPTION PHENOTYPE: Pale-green sepals and carpels and decreased
CC       chlorophyll levels. {ECO:0000269|PubMed:20305124}.
CC   -!- MISCELLANEOUS: Plants over-expressing PRE4 show long hypocotyls, pale
CC       green and slightly narrow leaves, elongated petioles and early
CC       flowering. They are not sensitive to the gibberellin inhibitor
CC       paclobutrazol during seed germination (PubMed:16527868).
CC       {ECO:0000305|PubMed:16527868}.
CC   -!- SIMILARITY: Belongs to the bHLH protein family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB41854.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL049746; CAB41854.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE78321.1; -; Genomic_DNA.
DR   PIR; T07710; T07710.
DR   RefSeq; NP_190355.2; NM_114639.3.
DR   AlphaFoldDB; F4JCN9; -.
DR   SMR; F4JCN9; -.
DR   BioGRID; 9245; 9.
DR   IntAct; F4JCN9; 9.
DR   STRING; 3702.AT3G47710.1; -.
DR   PaxDb; F4JCN9; -.
DR   PRIDE; F4JCN9; -.
DR   ProteomicsDB; 226410; -.
DR   EnsemblPlants; AT3G47710.1; AT3G47710.1; AT3G47710.
DR   GeneID; 823925; -.
DR   Gramene; AT3G47710.1; AT3G47710.1; AT3G47710.
DR   KEGG; ath:AT3G47710; -.
DR   Araport; AT3G47710; -.
DR   TAIR; locus:2100342; AT3G47710.
DR   eggNOG; ENOG502S4KP; Eukaryota.
DR   HOGENOM; CLU_183267_0_0_1; -.
DR   InParanoid; F4JCN9; -.
DR   OMA; ETCTHIM; -.
DR   OrthoDB; 1589357at2759; -.
DR   PRO; PR:F4JCN9; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; F4JCN9; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0009640; P:photomorphogenesis; IMP:TAIR.
DR   GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR   GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR044293; PRE.
DR   PANTHER; PTHR46446; PTHR46446; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   1: Evidence at protein level;
KW   Gibberellin signaling pathway; Growth regulation; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..92
FT                   /note="Transcription factor PRE4"
FT                   /id="PRO_0000429085"
FT   DOMAIN          5..60
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ   SEQUENCE   92 AA;  10360 MW;  DC39910FBA384ABD CRC64;
     MSSRKSRSRQ TGASMITDEQ INDLVLQLHR LLPELANNRR SGKVSASRVL QETCSYIRNL
     SKEVDDLSER LSQLLESTDS AQAALIRSLL MQ