PRE6_ARATH
ID PRE6_ARATH Reviewed; 94 AA.
AC Q8GW32;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Transcription factor PRE6;
DE AltName: Full=Basic helix-loop-helix protein 163;
DE Short=AtbHLH163;
DE Short=bHLH 163;
DE AltName: Full=Protein KIDARI;
DE AltName: Full=Protein PACLOBUTRAZOL RESISTANCE 6;
DE AltName: Full=bHLH transcription factor bHLH163;
GN Name=PRE6; Synonyms=BHLH163, KDR; OrderedLocusNames=At1g26945;
GN ORFNames=T2P11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, INTERACTION WITH HFR1, AND INDUCTION.
RX PubMed=16786307; DOI=10.1007/s11103-006-0010-2;
RA Hyun Y., Lee I.;
RT "KIDARI, encoding a non-DNA binding bHLH protein, represses light signal
RT transduction in Arabidopsis thaliana.";
RL Plant Mol. Biol. 61:283-296(2006).
RN [6]
RP FUNCTION, INTERACTION WITH HFR1, AND SUBCELLULAR LOCATION.
RX PubMed=23224238; DOI=10.1007/s10059-013-2159-2;
RA Hong S.Y., Seo P.J., Ryu J.Y., Cho S.H., Woo J.C., Park C.M.;
RT "A competitive peptide inhibitor KIDARI negatively regulates HFR1 by
RT forming nonfunctional heterodimers in Arabidopsis photomorphogenesis.";
RL Mol. Cells 35:25-31(2013).
CC -!- FUNCTION: Atypical and probable non DNA-binding bHLH transcription
CC factor that regulates light-mediated responses in day light conditions
CC by binding and inhibiting the activity of the bHLH transcription factor
CC HFR1, a critical regulator of light signaling and shade avoidance.
CC Forms non-functional heterodimers with HFR1, causing liberation and
CC activation of PIF4 from the transcriptionally inactive HFR1-PIF4
CC complex. {ECO:0000269|PubMed:16786307, ECO:0000269|PubMed:23224238}.
CC -!- SUBUNIT: Interacts with HFR1. {ECO:0000269|PubMed:16786307,
CC ECO:0000269|PubMed:23224238}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23224238}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:23224238}.
CC -!- INDUCTION: Circadian regulation with a peak of expression at midday.
CC {ECO:0000269|PubMed:16786307}.
CC -!- MISCELLANEOUS: Gain-of-function mutants kdr-D (T-DNA tagging) show long
CC hypocotyls, pale green and slightly narrow leaves, elongated petioles
CC and early flowering. They are not sensitive to the gibberellin
CC inhibitor paclobutrazol during seed germination (PubMed:16786307).
CC {ECO:0000305|PubMed:16786307}.
CC -!- SIMILARITY: Belongs to the bHLH protein family. {ECO:0000305}.
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DR EMBL; AC005508; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE30763.1; -; Genomic_DNA.
DR EMBL; AK119110; BAC43682.1; -; mRNA.
DR EMBL; BT004686; AAO42932.1; -; mRNA.
DR RefSeq; NP_849712.1; NM_179381.3.
DR AlphaFoldDB; Q8GW32; -.
DR SMR; Q8GW32; -.
DR BioGRID; 24820; 6.
DR IntAct; Q8GW32; 6.
DR STRING; 3702.AT1G26945.1; -.
DR PaxDb; Q8GW32; -.
DR PRIDE; Q8GW32; -.
DR ProteomicsDB; 236600; -.
DR EnsemblPlants; AT1G26945.1; AT1G26945.1; AT1G26945.
DR GeneID; 839585; -.
DR Gramene; AT1G26945.1; AT1G26945.1; AT1G26945.
DR KEGG; ath:AT1G26945; -.
DR Araport; AT1G26945; -.
DR TAIR; locus:1005716685; AT1G26945.
DR eggNOG; ENOG502S4KP; Eukaryota.
DR HOGENOM; CLU_183267_0_0_1; -.
DR InParanoid; Q8GW32; -.
DR OMA; DMDSDQA; -.
DR OrthoDB; 1589357at2759; -.
DR PhylomeDB; Q8GW32; -.
DR PRO; PR:Q8GW32; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GW32; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0009416; P:response to light stimulus; IMP:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR044293; PRE.
DR PANTHER; PTHR46446; PTHR46446; 1.
DR Pfam; PF00010; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Growth regulation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..94
FT /note="Transcription factor PRE6"
FT /id="PRO_0000429087"
FT DOMAIN 6..60
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 94 AA; 10718 MW; 33C277B0D8EA6BCC CRC64;
MSSRRSSRSR QSGSSRISDD QISDLVSKLQ HLIPELRRRR SDKVSASKVL QETCNYIRNL
HREVDDLSDR LSELLASTDD NSAEAAIIRS LLNY
