ID   ATG14_ASHGO             Reviewed;         308 AA.
AC   Q75BE5;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Autophagy-related protein 14;
GN   Name=ATG14; OrderedLocusNames=ADL381W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt) and
CC       autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex
CC       I. This complex is essential to recruit the ATG8-phosphatidylinositol
CC       conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal
CC       structure. ATG14 mediates the specific binding of the VPS34 PI3-kinase
CC       complex I to the preautophagosomal structure (PAS) (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Coiled-Coils at the N-terminal half are essential for
CC       autophagy. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG14 family. {ECO:0000305}.
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DR   EMBL; AE016817; AAS51539.1; -; Genomic_DNA.
DR   RefSeq; NP_983715.1; NM_209068.1.
DR   AlphaFoldDB; Q75BE5; -.
DR   SMR; Q75BE5; -.
DR   STRING; 33169.AAS51539; -.
DR   EnsemblFungi; AAS51539; AAS51539; AGOS_ADL381W.
DR   GeneID; 4619850; -.
DR   KEGG; ago:AGOS_ADL381W; -.
DR   eggNOG; ENOG502RY86; Eukaryota.
DR   HOGENOM; CLU_069448_0_0_1; -.
DR   InParanoid; Q75BE5; -.
DR   OMA; MYCSHCI; -.
DR   Proteomes; UP000000591; Chromosome IV.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016236; P:macroautophagy; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR023261; Autophagy-related_protein_14.
DR   PRINTS; PR02030; AUTOPHGYRP14.
PE   3: Inferred from homology;
KW   Autophagy; Coiled coil; Membrane; Protein transport; Reference proteome;
KW   Transport; Vacuole.
FT   CHAIN           1..308
FT                   /note="Autophagy-related protein 14"
FT                   /id="PRO_0000212450"
FT   REGION          3..18
FT                   /note="Cysteine repeats"
FT                   /evidence="ECO:0000250"
FT   COILED          73..109
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   308 AA;  35721 MW;  3F77A6C921E13E62 CRC64;
     MQCTICHRPS TVWYCAHCVN TSPKLILRYK LELTQICEEV TEMRDIVTST LENAISEKEG
     LLGKHMERLQ HLRLKRYNAR LSHRARELEQ HLDSKLSRRD GLRRALKQLS PDVAVVPAED
     PDEYRELRHK LTLLQNVVSM KSTQKFEELC QWFVFTCSTT EDDHFPYSIR FIPVCNIRNW
     RLLSTAQESL QHMCEFVIYA SRALLVDIPF GSHSEKLTTD HIAAVSHFTV NLLTILIKRK
     RLQERPDVPD LLGRYDIDGL LYLLCSGGDV ESITGTCPPT YKVVHEFVRT ALEDGDQSEE
     RGHWMVLE