ID   ATG14_CANGA             Reviewed;         364 AA.
AC   Q6FX35;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Autophagy-related protein 14;
GN   Name=ATG14; OrderedLocusNames=CAGL0C00627g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt) and
CC       autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex
CC       I. This complex is essential to recruit the ATG8-phosphatidylinositol
CC       conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal
CC       structure. ATG14 mediates the specific binding of the VPS34 PI3-kinase
CC       complex I to the preautophagosomal structure (PAS) (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Coiled-Coils at the N-terminal half are essential for
CC       autophagy. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG14 family. {ECO:0000305}.
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DR   EMBL; CR380949; CAG58113.1; -; Genomic_DNA.
DR   RefSeq; XP_445209.1; XM_445209.1.
DR   AlphaFoldDB; Q6FX35; -.
DR   SMR; Q6FX35; -.
DR   STRING; 5478.XP_445209.1; -.
DR   EnsemblFungi; CAG58113; CAG58113; CAGL0C00627g.
DR   GeneID; 2886881; -.
DR   KEGG; cgr:CAGL0C00627g; -.
DR   CGD; CAL0127632; ATG14.
DR   VEuPathDB; FungiDB:CAGL0C00627g; -.
DR   eggNOG; ENOG502RY86; Eukaryota.
DR   HOGENOM; CLU_069448_0_0_1; -.
DR   InParanoid; Q6FX35; -.
DR   OMA; MYCSHCI; -.
DR   Proteomes; UP000002428; Chromosome C.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; IEA:EnsemblFungi.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0034271; C:phosphatidylinositol 3-kinase complex, class III, type I; IEA:EnsemblFungi.
DR   GO; GO:0120095; C:vacuole-isolation membrane contact site; IEA:EnsemblFungi.
DR   GO; GO:0030242; P:autophagy of peroxisome; IEA:EnsemblFungi.
DR   GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IEA:EnsemblFungi.
DR   GO; GO:0016236; P:macroautophagy; IEA:EnsemblFungi.
DR   GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IEA:EnsemblFungi.
DR   InterPro; IPR023261; Autophagy-related_protein_14.
DR   InterPro; IPR018791; UV_resistance/autophagy_Atg14.
DR   Pfam; PF10186; ATG14; 1.
DR   PRINTS; PR02030; AUTOPHGYRP14.
PE   3: Inferred from homology;
KW   Autophagy; Coiled coil; Membrane; Protein transport; Reference proteome;
KW   Transport; Vacuole.
FT   CHAIN           1..364
FT                   /note="Autophagy-related protein 14"
FT                   /id="PRO_0000212451"
FT   REGION          5..20
FT                   /note="Cysteine repeats"
FT                   /evidence="ECO:0000250"
FT   COILED          38..114
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   364 AA;  42604 MW;  316BF71569E6F730 CRC64;
     MGMQCPICET QSHVFYCAHC INSSPDLLIR LKFDLYILGK INNALRNKVD QYLEEVDDEL
     NTNIRNGEQL ESNIGVQILK ERLGKVQVLR KERQNNKIKH KISQQDRRIK EKSRYIAELR
     SLINGPPKNQ NRFTDPQTIL KAQKQLQDKL EIAKRLSIQT RSEKLAMLNK WYSIRKRDSH
     DIPFTISYQP VISLKNFNRL PLPLIEGSLR KLIQYMNLLE HIYCIKYPSA MFVHEDEMLS
     LQGHSAKLKR NAPELLQVLI KLIQMILVVM TRSKLIDERK QNIDYAWILD QYDIDGLFYH
     MAMSIPIDTK SGAKNIQWST DRLVDIVCSS LGAEKEEVLL KDHKKLEPPT DKKYEVSDRW
     YIVG