ID   PRE_STRAG               Reviewed;         494 AA.
AC   P13925;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Plasmid recombination enzyme;
DE   AltName: Full=Mobilization protein;
GN   Name=pre; Synonyms=mob;
OS   Streptococcus agalactiae.
OG   Plasmid pMV158.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=1311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2677995; DOI=10.1093/nar/17.18.7283;
RA   van der Lelie D., Bron S., Venema G., Oskam L.;
RT   "Similarity of minus origins of replication and flanking open reading
RT   frames of plasmids pUB110, pTB913 and pMV158.";
RL   Nucleic Acids Res. 17:7283-7294(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2768188; DOI=10.1128/jb.171.9.4778-4784.1989;
RA   Priebe S.D., Lacks S.A.;
RT   "Region of the streptococcal plasmid pMV158 required for conjugative
RT   mobilization.";
RL   J. Bacteriol. 171:4778-4784(1989).
CC   -!- FUNCTION: The interaction of the RSA site and the PRE protein may not
CC       only serves a function in plasmid maintenance, but may also contributes
CC       to the distribution of small antibiotic resistance plasmids among Gram-
CC       positive bacteria.
CC   -!- MISCELLANEOUS: Contains conserved positively charged amino acids
CC       probably involved in the binding of the pre protein to the RSA site.
CC   -!- SIMILARITY: Belongs to the plasmid mobilization pre family.
CC       {ECO:0000305}.
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DR   EMBL; X15669; CAA33713.1; -; Genomic_DNA.
DR   EMBL; M28538; AAA25387.1; -; Genomic_DNA.
DR   PIR; A33952; A33952.
DR   PIR; S10134; S10134.
DR   RefSeq; WP_012218462.1; NC_010096.1.
DR   RefSeq; YP_001586274.1; NC_010096.1.
DR   PDB; 4LVI; X-ray; 1.90 A; A=2-199.
DR   PDB; 4LVJ; X-ray; 2.17 A; A=2-199.
DR   PDB; 4LVK; X-ray; 2.37 A; A=2-199.
DR   PDB; 4LVL; X-ray; 2.20 A; A=2-199.
DR   PDB; 4LVM; X-ray; 3.10 A; A/C=2-199.
DR   PDB; 5N2Q; X-ray; 2.00 A; A=2-198.
DR   PDBsum; 4LVI; -.
DR   PDBsum; 4LVJ; -.
DR   PDBsum; 4LVK; -.
DR   PDBsum; 4LVL; -.
DR   PDBsum; 4LVM; -.
DR   PDBsum; 5N2Q; -.
DR   AlphaFoldDB; P13925; -.
DR   SMR; P13925; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   InterPro; IPR001668; Mob_Pre.
DR   Pfam; PF01076; Mob_Pre; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Plasmid.
FT   CHAIN           1..494
FT                   /note="Plasmid recombination enzyme"
FT                   /id="PRO_0000068425"
FT   BINDING         44
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
FT   BINDING         115
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        213
FT                   /note="P -> L (in Ref. 2; AAA25387)"
FT                   /evidence="ECO:0000305"
FT   STRAND          5..11
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   HELIX           13..23
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   STRAND          27..29
FT                   /evidence="ECO:0007829|PDB:4LVL"
FT   HELIX           37..42
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   STRAND          44..47
FT                   /evidence="ECO:0007829|PDB:4LVK"
FT   STRAND          51..53
FT                   /evidence="ECO:0007829|PDB:4LVM"
FT   HELIX           55..66
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   STRAND          80..86
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   HELIX           89..93
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   HELIX           97..115
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   HELIX           117..119
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   STRAND          120..126
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   STRAND          128..131
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   STRAND          133..138
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   HELIX           148..151
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   HELIX           154..170
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   HELIX           189..194
FT                   /evidence="ECO:0007829|PDB:4LVI"
FT   TURN            195..197
FT                   /evidence="ECO:0007829|PDB:4LVM"
SQ   SEQUENCE   494 AA;  57859 MW;  91CB07E698AF6D99 CRC64;
     MSYMVARMQK MKAGNLGGAF KHNERVFETH SNKDINPSRS HLNYELTDRD RSVSYEKQIK
     DYVNENKVSN RAIRKDAVLC DEWIITSDKD FFEKLDEEQT RTFFETAKNY FAENYGESNI
     AYASVHLDES TPHMHMGVVP FENGKLSSKA MFDREELKHI QEDLPRYMSD HGFELERGKL
     NSEAKHKTVA EFKRAMADME LKEELLEKYH APPFVDERTG ELNNDTEAFW HEKEFADMFE
     VQSPIRETTN QEKMDWLRKQ YQEELKKLES SKKPLEDDLS HLEELLDKKT KEYIKIDSEA
     SERASELSKA EGYINTLENH SKSLEAKIEC LESDNLQLEK QKATKLEAKA LNESELRELK
     PKKNFLGKEH YELSPEQFEG LKAEVYRSRT LLHHKDIELE QAKRQVSLRA SKNYFTASLE
     RAKEKAKGES IDRLKSEIKR LKNENSILRQ QNDKMLGKLR ELMPDKAFKN LLSELKAIKP
     IVNIIKKAIE KSLF