ID   ATG14_KLULA             Reviewed;         307 AA.
AC   Q6CRH0;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Autophagy-related protein 14;
GN   Name=ATG14; OrderedLocusNames=KLLA0D09130g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt) and
CC       autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex
CC       I. This complex is essential to recruit the ATG8-phosphatidylinositol
CC       conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal
CC       structure. ATG14 mediates the specific binding of the VPS34 PI3-kinase
CC       complex I to the preautophagosomal structure (PAS) (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Coiled-Coils at the N-terminal half are essential for
CC       autophagy. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG14 family. {ECO:0000305}.
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DR   EMBL; CR382124; CAH00565.1; -; Genomic_DNA.
DR   RefSeq; XP_453469.1; XM_453469.1.
DR   AlphaFoldDB; Q6CRH0; -.
DR   SMR; Q6CRH0; -.
DR   STRING; 28985.XP_453469.1; -.
DR   EnsemblFungi; CAH00565; CAH00565; KLLA0_D09130g.
DR   GeneID; 2893581; -.
DR   KEGG; kla:KLLA0_D09130g; -.
DR   eggNOG; ENOG502RY86; Eukaryota.
DR   HOGENOM; CLU_069448_0_0_1; -.
DR   InParanoid; Q6CRH0; -.
DR   OMA; MYCSHCI; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016236; P:macroautophagy; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR023261; Autophagy-related_protein_14.
DR   InterPro; IPR018791; UV_resistance/autophagy_Atg14.
DR   Pfam; PF10186; ATG14; 1.
DR   PRINTS; PR02030; AUTOPHGYRP14.
PE   3: Inferred from homology;
KW   Autophagy; Coiled coil; Membrane; Protein transport; Reference proteome;
KW   Transport; Vacuole.
FT   CHAIN           1..307
FT                   /note="Autophagy-related protein 14"
FT                   /id="PRO_0000212452"
FT   REGION          4..22
FT                   /note="Cysteine repeats"
FT                   /evidence="ECO:0000250"
FT   COILED          36..113
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   307 AA;  35967 MW;  11C0EC36DF1FBCDE CRC64;
     MIHCGICGSP KIPNVEFLCA HCINGSPSIL LRDKLKLLLL RQEVEQLKHD VGEQLEAGFA
     GNGQFGKQLQ KLDVYNEKRR LIKLRQRVQL VKNKIDVKRN RYQELERYLK DEDHFDKDKK
     VFDVEEIVND EALTMSKLSQ VLQRKQTALF KELCQWFRIE KCDEDVNNNN NVSAYTIWGV
     PMINLKYSNE MDRDKQVISM RYIHQFINFS FNIWLFEGIS DVSIENEQNV IQNYSQLIYD
     TLRLLQVRKL VSESVSIRDI LIRYDLDGMI YYLSKSKYLS SLDDASNSYP PTLQNIKQLV
     ASMIPSI