PRFA_POLPE
ID PRFA_POLPE Reviewed; 287 AA.
AC P21259;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 3.
DT 25-MAY-2022, entry version 60.
DE RecName: Full=Pol-RFamide neuropeptides;
DE Contains:
DE RecName: Full=Pol-RFamide-II;
DE Contains:
DE RecName: Full=Pol-RFamide-I;
DE Contains:
DE RecName: Full=Neuropeptide;
DE Flags: Precursor;
OS Polyorchis penicillatus (Hydromedusa).
OC Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroidolina; Anthoathecata;
OC Capitata; Polyorchidae; Polyorchis.
OX NCBI_TaxID=6091;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PYROGLUTAMATE FORMATION AT GLN-53; GLN-65;
RP GLN-78; GLN-91; GLN-104; GLN-117; GLN-130; GLN-143; GLN-169; GLN-182;
RP GLN-195; GLN-208 AND GLN-221, AND AMIDATION AT PHE-59; PHE-71; PHE-84;
RP PHE-97; PHE-110; PHE-123; PHE-136; PHE-149; PHE-175; PHE-188; HIS-201;
RP PHE-214 AND PHE-227.
RX PubMed=7909659; DOI=10.1042/bj2990431;
RA Schmutzler C., Diekhoff D., Grimmelikhuijzen C.J.P.;
RT "The primary structure of the Pol-RFamide neuropeptide precursor protein
RT from the hydromedusa Polyorchis penicillatus indicates a novel processing
RT proteinase activity.";
RL Biochem. J. 299:431-436(1994).
RN [2]
RP PROTEIN SEQUENCE OF 182-188, PYROGLUTAMATE FORMATION AT GLN-182, AND
RP AMIDATION AT PHE-188.
RX PubMed=2905621; DOI=10.1016/0006-8993(88)90219-3;
RA Grimmelikhuijzen C.J.P., Hahn M., Rinehart K.L. Jr., Spencer A.N.;
RT "Isolation of pyroGlu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide), a novel
RT neuropeptide from hydromedusae.";
RL Brain Res. 475:198-203(1988).
CC -!- FUNCTION: Has direct action on motoneurons, and effect includes
CC transient inhibition followed by prolonged excitation.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- PTM: The N-terminal processing sites of the Pol-RFamide peptides are
CC acidic suggesting that cniderian nervous systems may use a variety of
CC unconventional processing procedures.
CC -!- SIMILARITY: Belongs to the FARP (FMRFamide related peptide) family.
CC {ECO:0000305}.
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DR EMBL; L14777; AAA20898.1; -; mRNA.
DR PIR; S43852; S43852.
DR AlphaFoldDB; P21259; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR002544; FMRFamid-related_peptide-like.
DR Pfam; PF01581; FARP; 12.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Pyrrolidone carboxylic acid; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT PROPEP 22..52
FT /evidence="ECO:0000269|PubMed:2905621"
FT /id="PRO_0000009753"
FT PEPTIDE 53..59
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009754"
FT PROPEP 62..64
FT /id="PRO_0000009755"
FT PEPTIDE 65..71
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009756"
FT PROPEP 74..77
FT /id="PRO_0000009757"
FT PEPTIDE 78..84
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009758"
FT PROPEP 87..90
FT /id="PRO_0000009759"
FT PEPTIDE 91..97
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009760"
FT PROPEP 100..103
FT /id="PRO_0000009761"
FT PEPTIDE 104..110
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009762"
FT PROPEP 113..116
FT /id="PRO_0000009763"
FT PEPTIDE 117..123
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009764"
FT PROPEP 126..129
FT /id="PRO_0000009765"
FT PEPTIDE 130..136
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009766"
FT PROPEP 139..142
FT /id="PRO_0000009767"
FT PEPTIDE 143..149
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009768"
FT PROPEP 152..168
FT /id="PRO_0000009769"
FT PEPTIDE 169..175
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009770"
FT PROPEP 178..181
FT /id="PRO_0000009771"
FT PEPTIDE 182..188
FT /note="Pol-RFamide-I"
FT /id="PRO_0000009772"
FT PROPEP 192..194
FT /id="PRO_0000009773"
FT PEPTIDE 195..201
FT /note="Neuropeptide"
FT /id="PRO_0000009774"
FT PROPEP 204..207
FT /id="PRO_0000009775"
FT PEPTIDE 208..214
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009776"
FT PROPEP 217..220
FT /id="PRO_0000009777"
FT PEPTIDE 221..227
FT /note="Pol-RFamide-II"
FT /id="PRO_0000009778"
FT PROPEP 230..287
FT /id="PRO_0000009779"
FT REGION 229..267
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..255
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 53
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 59
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 65
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 71
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 78
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 84
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 91
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 97
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 104
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 110
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 117
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 123
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 130
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 136
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 143
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 149
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 169
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 175
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 182
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:2905621,
FT ECO:0000269|PubMed:7909659"
FT MOD_RES 188
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:2905621,
FT ECO:0000269|PubMed:7909659"
FT MOD_RES 195
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 201
FT /note="Histidine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 208
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 214
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 221
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:7909659"
FT MOD_RES 227
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:7909659"
SQ SEQUENCE 287 AA; 33531 MW; FDF1F52C47D4BB11 CRC64;
MNLITLLVLG VSTCLIYGIE ADEKTSSALE NEIVEILNGN FKNEKKSIET SDQWLKGRFG
REVNQWLKGR FGRELSDQWL KGRFGRELSD QWLKGRFGRE VLDQWLKGRF GRDASNQWLK
GRFGRELSDQ WLKGRFGREG SNQWLKGRFG REASKNDLEK QNGRGDSDQW LKGRFGREAR
KQLLGGRFGR KDMNQLLAER HGRETSDQWL KGRFGRQLSD QWLKGRFGRE VKNDKNNPFR
SRYTGDSTQL QRENNQPIEE LRDNTEKVSI ENKPIMKKTS VKISKTV
