PRFB2_ARATH
ID PRFB2_ARATH Reviewed; 482 AA.
AC F4I532; Q9C7K9; Q9C7X9;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Peptide chain release factor PrfB2, chloroplastic {ECO:0000303|PubMed:21771930};
DE Short=AtPrfB2 {ECO:0000303|PubMed:21771930};
DE Flags: Precursor;
GN Name=PRFB2 {ECO:0000303|PubMed:21771930};
GN OrderedLocusNames=At1g56350 {ECO:0000312|Araport:AT1G56350};
GN ORFNames=F13N6.6 {ECO:0000312|EMBL:AAG51510.1},
GN F14G9.3 {ECO:0000312|EMBL:AAG50902.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NOMENCLATURE.
RX PubMed=21771930; DOI=10.1105/tpc.111.085324;
RA Stoppel R., Lezhneva L., Schwenkert S., Torabi S., Felder S., Meierhoff K.,
RA Westhoff P., Meurer J.;
RT "Recruitment of a ribosomal release factor for light- and stress-dependent
RT regulation of petB transcript stability in Arabidopsis chloroplasts.";
RL Plant Cell 23:2680-2695(2011).
CC -!- FUNCTION: Directs the termination of translation in response to the
CC peptide chain termination codon UGA. Required for the proper
CC translation, stability and normal processing of UGA-containing
CC polycistronic transcripts in chloroplasts.
CC {ECO:0000250|UniProtKB:Q9LVY0}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000250|UniProtKB:Q9LVY0}.
CC -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50902.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG51510.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC058785; AAG51510.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC069159; AAG50902.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33382.1; -; Genomic_DNA.
DR PIR; E96605; E96605.
DR RefSeq; NP_176032.2; NM_104515.4.
DR AlphaFoldDB; F4I532; -.
DR SMR; F4I532; -.
DR STRING; 3702.AT1G56350.1; -.
DR PaxDb; F4I532; -.
DR PRIDE; F4I532; -.
DR ProteomicsDB; 234872; -.
DR EnsemblPlants; AT1G56350.1; AT1G56350.1; AT1G56350.
DR GeneID; 842089; -.
DR Gramene; AT1G56350.1; AT1G56350.1; AT1G56350.
DR KEGG; ath:AT1G56350; -.
DR Araport; AT1G56350; -.
DR TAIR; locus:2010748; AT1G56350.
DR eggNOG; KOG2726; Eukaryota.
DR HOGENOM; CLU_036856_2_1_1; -.
DR InParanoid; F4I532; -.
DR OMA; NKPDLWD; -.
DR OrthoDB; 1313171at2759; -.
DR PRO; PR:F4I532; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I532; baseline and differential.
DR Genevisible; F4I532; AT.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0016149; F:translation release factor activity, codon specific; IEA:InterPro.
DR HAMAP; MF_00094; Rel_fac_2; 1.
DR InterPro; IPR005139; PCRF.
DR InterPro; IPR000352; Pep_chain_release_fac_I.
DR InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR InterPro; IPR004374; PrfB.
DR Pfam; PF03462; PCRF; 1.
DR Pfam; PF00472; RF-1; 1.
DR SMART; SM00937; PCRF; 1.
DR SUPFAM; SSF75620; SSF75620; 1.
DR TIGRFAMs; TIGR00020; prfB; 1.
PE 3: Inferred from homology;
KW Chloroplast; Plastid; Protein biosynthesis; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..21
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 22..482
FT /note="Peptide chain release factor PrfB2, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000430965"
SQ SEQUENCE 482 AA; 53427 MW; 3B30087EF66D202E CRC64;
MLSLIIRRSR SRFIIHGIKI SCNSLSAVDS SSSVLVSRRT FSSTPALSYF GFNYGKISNL
NQRFGNYAYS GSSFASSTRS LSSEAVAVAA TCDGLTVERI IANQWPILDE NEGDWKSHAA
AIAQSIQVIK RRLQWKKLLV RLKVLSAELN KSDLWDDPTH AGKISREHGS LTGKMKGVMT
FERELLEHID MLKLAKEEND SELESETLKA LIDMRRVSKE KELEALLSAD NDPCSCYIEV
QAGAGGTESN DWAAMVMEMY KTWAQRRKFS VTVVDEAPGE IAGIKRATIK VNGEYAYGYA
KAEVGVHRLV RISPFDSGKR RHTSFAAVAV IPILGDGSTR VEINDSDLRI ERFRSGGAGG
QHANTTDSAV RIVHIPTGIT ATCQNERSQH SNKASAMAVL QSRLDQLEMA RQTAMNAQHT
QSLTEISWGN QIRTYVLHPY RMVKDLRTNY EVSDPDSVLE GDLDGFILSF LSSSLDKDDP
EH
