ID   PRFB3_ARATH             Reviewed;         406 AA.
AC   F4J264; Q8L7M2; Q9M2M8;
DT   26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Peptide chain release factor PrfB3, chloroplastic {ECO:0000303|PubMed:21771930};
DE            Short=AtPrfB3 {ECO:0000303|PubMed:21771930};
DE   Flags: Precursor;
GN   Name=PRFB3 {ECO:0000303|PubMed:21771930};
GN   OrderedLocusNames=At3g57190 {ECO:0000312|Araport:AT3G57190};
GN   ORFNames=F28O9.40 {ECO:0000312|EMBL:CAB68125.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-406.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=21771930; DOI=10.1105/tpc.111.085324;
RA   Stoppel R., Lezhneva L., Schwenkert S., Torabi S., Felder S., Meierhoff K.,
RA   Westhoff P., Meurer J.;
RT   "Recruitment of a ribosomal release factor for light- and stress-dependent
RT   regulation of petB transcript stability in Arabidopsis chloroplasts.";
RL   Plant Cell 23:2680-2695(2011).
RN   [5]
RP   INTERACTION WITH PDE338, AND SUBCELLULAR LOCATION.
RX   PubMed=30962391; DOI=10.1105/tpc.18.00946;
RA   Jiang J., Chai X., Manavski N., Williams-Carrier R., He B., Brachmann A.,
RA   Ji D., Ouyang M., Liu Y., Barkan A., Meurer J., Zhang L., Chi W.;
RT   "An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA
RT   Stability and Translation in Arabidopsis and Maize.";
RL   Plant Cell 31:1308-1327(2019).
CC   -!- FUNCTION: Involved in the light- and stress-dependent regulation of
CC       stability of 3' processed petB transcripts, thus regulating cytochrome
CC       b6 accumulation, a rate-limiting step in photosynthetic electron
CC       transport. May be recruited to specifically protect petB transcripts
CC       against 3'-5' exonucleolytic attack by masking the 3' ends. Does not
CC       function as release factor. {ECO:0000269|PubMed:21771930}.
CC   -!- SUBUNIT: Interacts with PDE338. {ECO:0000269|PubMed:30962391}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC       {ECO:0000269|PubMed:21771930}. Plastid, chloroplast
CC       {ECO:0000269|PubMed:30962391}.
CC   -!- DISRUPTION PHENOTYPE: High chlorophyll fluorescence (hcf) phenotype and
CC       plant death at seedling stage when homozygous.
CC       {ECO:0000269|PubMed:21771930}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000305}.
CC   -!- CAUTION: Lacks the stop codon recognition motif 'SPF' and the catalytic
CC       center 'GGQ' for peptidyl-tRNA hydrolysis, which are two conserved
CC       features of the family. {ECO:0000305|PubMed:21771930}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM91577.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=CAB68125.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL137080; CAB68125.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE79625.1; -; Genomic_DNA.
DR   EMBL; AY128374; AAM91577.1; ALT_INIT; mRNA.
DR   EMBL; BT008450; AAP37809.1; -; mRNA.
DR   PIR; T45797; T45797.
DR   RefSeq; NP_191278.2; NM_115579.3.
DR   AlphaFoldDB; F4J264; -.
DR   SMR; F4J264; -.
DR   STRING; 3702.AT3G57190.1; -.
DR   PaxDb; F4J264; -.
DR   PRIDE; F4J264; -.
DR   ProteomicsDB; 234804; -.
DR   EnsemblPlants; AT3G57190.1; AT3G57190.1; AT3G57190.
DR   GeneID; 824886; -.
DR   Gramene; AT3G57190.1; AT3G57190.1; AT3G57190.
DR   KEGG; ath:AT3G57190; -.
DR   Araport; AT3G57190; -.
DR   TAIR; locus:2082563; AT3G57190.
DR   eggNOG; KOG2726; Eukaryota.
DR   HOGENOM; CLU_036856_6_2_1; -.
DR   InParanoid; F4J264; -.
DR   OMA; AGMDSIN; -.
DR   OrthoDB; 1313171at2759; -.
DR   PRO; PR:F4J264; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; F4J264; baseline and differential.
DR   Genevisible; F4J264; AT.
DR   GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR   GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR   GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:TAIR.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR   GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR   GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR   GO; GO:0043488; P:regulation of mRNA stability; IMP:TAIR.
DR   GO; GO:0006415; P:translational termination; IEA:InterPro.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   Pfam; PF03462; PCRF; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; SSF75620; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Growth regulation; Plastid; Reference proteome; RNA-binding;
KW   Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000305"
FT   CHAIN           ?..406
FT                   /note="Peptide chain release factor PrfB3, chloroplastic"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000430966"
SQ   SEQUENCE   406 AA;  45969 MW;  324723C9CD6F78DC CRC64;
     MAAKIIGGCC SWRRFYRKRT SSRFLIFSVR ASSSMDDMDT VYKQLGLFSL KKKIKDVVLK
     AEMFAPDALE LEEEQWIKQE ETMRYFDLWD DPAKSDEILL KLADRAKAVD SLKDLKYKAE
     EAKLIIQLGE MDAIDYSLFE QAYDSSLDVS RSLHHYEMSK LLRDQYDAEG ACMIIKSGSP
     GAKSQIWTEQ VVSMYIKWAE RLGQNARVAE KCSLLSNKSG VSSATIEFEF EFAYGYLLGE
     RGVHRLIISS TSNEECSATV DIIPLFLRAS PDFEVKEGDL IVSYPAKEDH KIAENMVCIH
     HIPSGVTLQS SGERNRFANR IKALNRLKAK LLVIAKEQKV SDVNKIDSKN ILEPREETRS
     YVSKGHKMVV DRKTGLEILD LKSVLDGNIG PLLGAHISMR RSIDAI