PRG2_MOUSE
ID PRG2_MOUSE Reviewed; 223 AA.
AC Q61878;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Bone marrow proteoglycan;
DE Short=BMPG;
DE AltName: Full=Proteoglycan 2;
DE Contains:
DE RecName: Full=Eosinophil granule major basic protein;
DE Short=EMBP;
DE Short=MBP;
DE Flags: Precursor;
GN Name=Prg2; Synonyms=Mbp-1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=CBA/J; TISSUE=Liver;
RX PubMed=7673718;
RA Larson K.A., Horton M.A., Madden B.J., Gleich G.J., Lee N.A., Lee J.J.;
RT "The identification and cloning of a murine major basic protein gene
RT expressed in eosinophils.";
RL J. Immunol. 155:3002-3012(1995).
RN [2]
RP NITRATION.
RX PubMed=18694936; DOI=10.1074/jbc.m801196200;
RA Ulrich M., Petre A., Youhnovski N., Proemm F., Schirle M., Schumm M.,
RA Pero R.S., Doyle A., Checkel J., Kita H., Thiyagarajan N., Acharya K.R.,
RA Schmid-Grendelmeier P., Simon H.-U., Schwarz H., Tsutsui M., Shimokawa H.,
RA Bellon G., Lee J.J., Przybylski M., Doering G.;
RT "Post-translational tyrosine nitration of eosinophil granule toxins
RT mediated by eosinophil peroxidase.";
RL J. Biol. Chem. 283:28629-28640(2008).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Cytotoxin and helminthotoxin. MBP also induces non-cytolytic
CC histamine release from basophils. It is involved in antiparasitic
CC defense mechanisms and immune hypersensitivity reactions (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic granule {ECO:0000250}. Note=Matrix of
CC eosinophil's large specific granule (crystalloid core). {ECO:0000250}.
CC -!- PTM: Nitrated. {ECO:0000269|PubMed:18694936}.
CC -!- WEB RESOURCE: Name=Functional Glycomics Gateway - Glycan Binding;
CC Note=Eosinophil major basic protein;
CC URL="http://www.functionalglycomics.org/glycomics/GBPServlet?&operationType=view&cbpId=cbp_mou_Ctlect_151";
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DR EMBL; L46768; AAA83027.1; -; Genomic_DNA.
DR CCDS; CCDS16198.1; -.
DR RefSeq; NP_032946.1; NM_008920.4.
DR AlphaFoldDB; Q61878; -.
DR SMR; Q61878; -.
DR BioGRID; 202360; 1.
DR STRING; 10090.ENSMUSP00000028467; -.
DR MEROPS; I63.001; -.
DR CarbonylDB; Q61878; -.
DR PhosphoSitePlus; Q61878; -.
DR jPOST; Q61878; -.
DR MaxQB; Q61878; -.
DR PaxDb; Q61878; -.
DR PRIDE; Q61878; -.
DR ProteomicsDB; 289406; -.
DR DNASU; 19074; -.
DR Ensembl; ENSMUST00000028467; ENSMUSP00000028467; ENSMUSG00000027073.
DR GeneID; 19074; -.
DR KEGG; mmu:19074; -.
DR UCSC; uc008kjp.1; mouse.
DR CTD; 5553; -.
DR MGI; MGI:103294; Prg2.
DR VEuPathDB; HostDB:ENSMUSG00000027073; -.
DR eggNOG; KOG4297; Eukaryota.
DR GeneTree; ENSGT00440000039859; -.
DR HOGENOM; CLU_107200_1_0_1; -.
DR InParanoid; Q61878; -.
DR OMA; WGRCKRF; -.
DR OrthoDB; 1328472at2759; -.
DR PhylomeDB; Q61878; -.
DR TreeFam; TF336281; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 19074; 2 hits in 75 CRISPR screens.
DR PRO; PR:Q61878; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q61878; protein.
DR Bgee; ENSMUSG00000027073; Expressed in femorotibial joint and 67 other tissues.
DR ExpressionAtlas; Q61878; baseline and differential.
DR Genevisible; Q61878; MM.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0002215; P:defense response to nematode; IMP:MGI.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0032693; P:negative regulation of interleukin-10 production; IMP:MGI.
DR GO; GO:0010936; P:negative regulation of macrophage cytokine production; IMP:MGI.
DR GO; GO:0032753; P:positive regulation of interleukin-4 production; IMP:MGI.
DR CDD; cd03598; CLECT_EMBP_like; 1.
DR Gene3D; 3.10.100.10; -; 1.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR033816; EMBP_CTLD.
DR InterPro; IPR002352; Eosinophil_major_basic.
DR Pfam; PF00059; Lectin_C; 1.
DR PRINTS; PR00770; EMAJORBASICP.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW Immunity; Lectin; Nitration; Reference proteome; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..223
FT /note="Bone marrow proteoglycan"
FT /id="PRO_0000259924"
FT PROPEP 17..106
FT /note="Acidic"
FT /id="PRO_0000017387"
FT CHAIN 107..223
FT /note="Eosinophil granule major basic protein"
FT /id="PRO_0000017388"
FT DOMAIN 124..223
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT REGION 20..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 55..69
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 126..221
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 198..213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
SQ SEQUENCE 223 AA; 24255 MW; 7D66D946DCEADA00 CRC64;
MKFPLLLALL VGGASALHLS SETSDSKSPL MDENLPRDAE ISGPEGEECP PGEELMPLEG
EKEEGSGSEG VPGDEGAVSG QDVTDVDLQC PKEEDTTSLM GDSGCKTCRY LLVRRAECFD
KAQSVCRRCY RGTLASIHSF SVNFGIQSAV RGINQGQVWI GGRIKGWGRC KRFRWVDGSS
WNFAYWAAGQ PCPGGGRCVT LCTQGGHWRL SHCVKRRPFI CSY
