PRGK_SALTY
ID PRGK_SALTY Reviewed; 252 AA.
AC P41786;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Lipoprotein PrgK;
DE Flags: Precursor;
GN Name=prgK; OrderedLocusNames=STM2871;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 14028s / SGSG 2262;
RX PubMed=7476203; DOI=10.1111/j.1365-2958.1995.mmi_17010169.x;
RA Pegues D.A., Hantman M.J., Behlau I., Miller S.I.;
RT "PhoP/PhoQ transcriptional repression of Salmonella typhimurium invasion
RT genes: evidence for a role in protein secretion.";
RL Mol. Microbiol. 17:169-181(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Required for invasion of epithelial cells. Could be involved
CC in protein secretion.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the YscJ lipoprotein family. {ECO:0000305}.
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DR EMBL; U21676; AAB60191.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21751.1; -; Genomic_DNA.
DR PIR; S69786; S69786.
DR RefSeq; NP_461792.1; NC_003197.2.
DR RefSeq; WP_000621238.1; NC_003197.2.
DR PDB; 2Y9J; EM; 6.40 A; Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v=21-190.
DR PDB; 3J6D; EM; -; Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v=1-252.
DR PDB; 4OYC; X-ray; 2.60 A; A/B=96-200.
DR PDB; 4W4M; X-ray; 3.20 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=19-92.
DR PDB; 5TCP; EM; 4.30 A; 0/2/4/6/8/B/D/F/H/J/L/N/P/R/T/V/X/Z/a/c/e/g/i/k=18-252.
DR PDB; 5TCR; EM; 6.30 A; 1/3/5/7/9/P/R/T/V/X/a/c/e/g/i/k/m/o/q/s/u/w/y/z=18-252.
DR PDB; 6DUZ; EM; 3.60 A; Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v=1-252.
DR PDB; 6PEM; EM; 3.50 A; AA/AB/AC/AD/AE/AF/AG/AH/AI/AJ/AK/AL/o/p/q/r/s/t/u/v/w/x/y/z=1-252.
DR PDB; 6Q14; EM; 3.80 A; AA/AB/AC/AD/AE/AF/AG/AH/AI/AJ/AK/AL/o/p/q/r/s/t/u/v/w/x/y/z=1-252.
DR PDB; 6Q15; EM; 5.15 A; AA/AB/AC/AD/AE/AF/AG/AH/AI/AJ/AK/AL/o/p/q/r/s/t/u/v/w/x/y/z=1-252.
DR PDB; 6Q16; EM; 4.10 A; AA/AB/AC/AD/AE/AF/AG/AH/AI/AJ/AK/AL/o/p/q/r/s/t/u/v/w/x/y/z=1-252.
DR PDB; 6UOT; EM; 3.30 A; Y/Z/a/b/c/d/e/f/g/h/i/j/k/l/m/n/o/p/q/r/s/t/u/v=1-252.
DR PDB; 6UOV; EM; 3.50 A; A/C/E/G/I/K/M/O/Q/S/U/W/Y/a/c/e/g/i/k/m/o/q/s=1-252.
DR PDB; 7AH9; EM; 3.30 A; 6A/6B/6C/6D/6E/6F/6G/6H/6I/6J/6K/6L/6M/6N/6O/6P/6Q/6R/6S/6T/6U/6V/6W/6X=1-252.
DR PDB; 7AHI; EM; 3.30 A; 6A/6B/6C/6D/6E/6F/6G/6H/6I/6J/6K/6L/6M/6N/6O/6P/6Q/6R/6S/6T/6U/6V/6W/6X=1-252.
DR PDBsum; 2Y9J; -.
DR PDBsum; 3J6D; -.
DR PDBsum; 4OYC; -.
DR PDBsum; 4W4M; -.
DR PDBsum; 5TCP; -.
DR PDBsum; 5TCR; -.
DR PDBsum; 6DUZ; -.
DR PDBsum; 6PEM; -.
DR PDBsum; 6Q14; -.
DR PDBsum; 6Q15; -.
DR PDBsum; 6Q16; -.
DR PDBsum; 6UOT; -.
DR PDBsum; 6UOV; -.
DR PDBsum; 7AH9; -.
DR PDBsum; 7AHI; -.
DR AlphaFoldDB; P41786; -.
DR BMRB; P41786; -.
DR SMR; P41786; -.
DR DIP; DIP-59554N; -.
DR IntAct; P41786; 2.
DR STRING; 99287.STM2871; -.
DR TCDB; 3.A.6.1.3; the type iii (virulence-related) secretory pathway (iiisp) family.
DR PaxDb; P41786; -.
DR EnsemblBacteria; AAL21751; AAL21751; STM2871.
DR GeneID; 1254394; -.
DR KEGG; stm:STM2871; -.
DR PATRIC; fig|99287.12.peg.3027; -.
DR HOGENOM; CLU_073268_2_0_6; -.
DR OMA; IKYRESA; -.
DR PhylomeDB; P41786; -.
DR BioCyc; SENT99287:STM2871-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 3.30.300.30; -; 1.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR006182; FliF_N_dom.
DR InterPro; IPR003282; T3SS_HrcJ/YscJ.
DR InterPro; IPR043427; YscJ/FliF.
DR PANTHER; PTHR30046; PTHR30046; 1.
DR Pfam; PF01514; YscJ_FliF; 1.
DR PRINTS; PR01338; TYPE3OMKPROT.
DR TIGRFAMs; TIGR02544; III_secr_YscJ; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Lipoprotein; Membrane; Palmitate;
KW Protein transport; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix; Transport; Virulence.
FT SIGNAL 1..17
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 18..252
FT /note="Lipoprotein PrgK"
FT /id="PRO_0000018182"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT LIPID 18
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 18
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT STRAND 20..27
FT /evidence="ECO:0007829|PDB:4W4M"
FT HELIX 29..40
FT /evidence="ECO:0007829|PDB:4W4M"
FT TURN 41..43
FT /evidence="ECO:0007829|PDB:4W4M"
FT STRAND 47..50
FT /evidence="ECO:0007829|PDB:4W4M"
FT HELIX 52..54
FT /evidence="ECO:0007829|PDB:4W4M"
FT STRAND 56..61
FT /evidence="ECO:0007829|PDB:4W4M"
FT HELIX 62..64
FT /evidence="ECO:0007829|PDB:4W4M"
FT HELIX 65..74
FT /evidence="ECO:0007829|PDB:4W4M"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:6UOT"
FT STRAND 93..95
FT /evidence="ECO:0007829|PDB:6UOT"
FT HELIX 98..117
FT /evidence="ECO:0007829|PDB:4OYC"
FT STRAND 120..122
FT /evidence="ECO:0007829|PDB:6UOV"
FT STRAND 123..131
FT /evidence="ECO:0007829|PDB:4OYC"
FT TURN 136..138
FT /evidence="ECO:0007829|PDB:6UOT"
FT STRAND 147..154
FT /evidence="ECO:0007829|PDB:4OYC"
FT HELIX 160..174
FT /evidence="ECO:0007829|PDB:4OYC"
FT STRAND 175..177
FT /evidence="ECO:0007829|PDB:6UOT"
FT HELIX 180..182
FT /evidence="ECO:0007829|PDB:4OYC"
FT STRAND 183..188
FT /evidence="ECO:0007829|PDB:4OYC"
SQ SEQUENCE 252 AA; 28210 MW; 2C0A6FC06667E256 CRC64;
MIRRYLYTFL LVMTLAGCKD KDLLKGLDQE QANEVIAVLQ MHNIEANKID SGKLGYSITV
AEPDFTAAVY WIKTYQLPPR PRVEIAQMFP ADSLVSSPRA EKARLYSAIE QRLEQSLQTM
EGVLSARVHI SYDIDAGENG RPPKPVHLSA LAVYERGSPL AHQISDIKRF LKNSFADVDY
DNISVVLSER SDAQLQAPGT PVKRNSFATS WIVLIILLSV MSAGFGVWYY KNHYARNKKG
ITADDKAKSS NE
