ID   ATG14_SCHPO             Reviewed;         390 AA.
AC   O42862;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Autophagy-related protein 14;
GN   Name=atg14; ORFNames=SPAC25A8.02;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [3]
RP   IDENTIFICATION, INTERACTION WITH ATG6, AND SUBCELLULAR LOCATION.
RX   PubMed=23950735; DOI=10.1371/journal.pgen.1003715;
RA   Sun L.L., Li M., Suo F., Liu X.M., Shen E.Z., Yang B., Dong M.Q., He W.Z.,
RA   Du L.L.;
RT   "Global analysis of fission yeast mating genes reveals new autophagy
RT   factors.";
RL   PLoS Genet. 9:E1003715-E1003715(2013).
CC   -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt) and
CC       autophagy as a part of the autophagy-specific vps34 PI3-kinase complex
CC       I. This complex is essential to recruit the atg8-phosphatidylinositol
CC       conjugate and the atg12-atg5 conjugate to the preautophagosomal
CC       structure. Atg14 mediates the specific binding of the vps34 PI3-kinase
CC       complex I to the preautophagosomal structure (PAS) (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the autophagy-specific vps34 PI3-kinase complex I
CC       composed of ppk19/vps15, atg6, pik3/vps34 and atg14 (By similarity).
CC       Interacts with atg6. {ECO:0000250, ECO:0000269|PubMed:23950735}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Preautophagosomal structure membrane;
CC       Peripheral membrane protein. Vacuole membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Coiled-Coils at the N-terminal half are essential for
CC       autophagy. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG14 family. {ECO:0000305}.
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DR   EMBL; CU329670; CAA16952.1; -; Genomic_DNA.
DR   PIR; T38370; T38370.
DR   RefSeq; NP_593618.1; NM_001019049.2.
DR   AlphaFoldDB; O42862; -.
DR   SMR; O42862; -.
DR   BioGRID; 278187; 11.
DR   STRING; 4896.SPAC25A8.02.1; -.
DR   PaxDb; O42862; -.
DR   PRIDE; O42862; -.
DR   EnsemblFungi; SPAC25A8.02.1; SPAC25A8.02.1:pep; SPAC25A8.02.
DR   GeneID; 2541691; -.
DR   KEGG; spo:SPAC25A8.02; -.
DR   PomBase; SPAC25A8.02; atg14.
DR   VEuPathDB; FungiDB:SPAC25A8.02; -.
DR   HOGENOM; CLU_714017_0_0_1; -.
DR   OMA; ICIFLAQ; -.
DR   Reactome; R-SPO-1632852; Macroautophagy.
DR   PRO; PR:O42862; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005776; C:autophagosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0097629; C:extrinsic component of omegasome membrane; IBA:GO_Central.
DR   GO; GO:0097632; C:extrinsic component of phagophore assembly site membrane; IBA:GO_Central.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; ISO:PomBase.
DR   GO; GO:0044233; C:mitochondria-associated endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0000407; C:phagophore assembly site; IDA:PomBase.
DR   GO; GO:0035032; C:phosphatidylinositol 3-kinase complex, class III; IBA:GO_Central.
DR   GO; GO:0034271; C:phosphatidylinositol 3-kinase complex, class III, type I; IDA:PomBase.
DR   GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR   GO; GO:0009267; P:cellular response to starvation; IBA:GO_Central.
DR   GO; GO:0016236; P:macroautophagy; IMP:PomBase.
DR   GO; GO:0000423; P:mitophagy; IBA:GO_Central.
DR   GO; GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR018791; UV_resistance/autophagy_Atg14.
DR   Pfam; PF10186; ATG14; 1.
PE   1: Evidence at protein level;
KW   Autophagy; Coiled coil; Cytoplasm; Membrane; Protein transport;
KW   Reference proteome; Transport; Vacuole.
FT   CHAIN           1..390
FT                   /note="Autophagy-related protein 14"
FT                   /id="PRO_0000304106"
FT   REGION          7..21
FT                   /note="Cysteine repeats"
FT                   /evidence="ECO:0000250"
FT   COILED          31..83
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   390 AA;  45304 MW;  203FC1DD42238E09 CRC64;
     MLHVNKCQLC ETRRPSICES CLKKQLYDFY HKRDEFSRDI ESELEKVAKL KSESFSLKGK
     ITQKEELTYR LQNLAASLNE KKSLSCDLHS RKQLIEDDLS NRKKSINIAS QKLAGLSHST
     SDYFSKEYLT AYRRSELLQN KLHEYQKKLI TRVLDMYQLH WQRDLVLNTQ GCTQKSAQMG
     SEFNPDSIDS SLAYRLSKLS MGNSKSDLSH SDNETGHFYS NFFSQVMELN ASVTISGIPV
     CIRSKEKMFL NPDCALTLSF ICIFLAQYTS IPLPCPLQLP SPDQKPMTSF SSEQMLYIMY
     NIVWISWNCG IFSFPRGITQ SQLFELMQYT GFWLVQLRTK PLTSQKPDWH YKMGMDFDLF
     HRLYLARLPI PINYSSLRSR SSSKPYQLIS