ID   ATG14_YEAS7             Reviewed;         344 AA.
AC   A6ZL72;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Autophagy-related protein 14;
DE   AltName: Full=Cytoplasm to vacuole targeting protein 12;
GN   Name=ATG14; Synonyms=APG14, CVT12; ORFNames=SCY_0342;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt) and
CC       autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex
CC       I. This complex is essential to recruit the ATG8-phosphatidylinositol
CC       conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal
CC       structure. ATG14 mediates the specific binding of the VPS34 PI3-kinase
CC       complex I to the preautophagosomal structure (PAS) (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the autophagy-specific VPS34 PI3-kinase complex I
CC       composed of VPS15, VPS30, VPS34 and ATG14. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Vacuole
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC       Note=TRS85 is required for the recruitment of ATG14 to the PAS.
CC       {ECO:0000250}.
CC   -!- DOMAIN: Coiled-Coils at the N-terminal half are essential for
CC       interaction with VPS30 and VPS34 and autophagy. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG14 family. {ECO:0000305}.
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DR   EMBL; AAFW02000011; EDN64741.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZL72; -.
DR   SMR; A6ZL72; -.
DR   EnsemblFungi; EDN64741; EDN64741; SCY_0342.
DR   HOGENOM; CLU_069448_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016236; P:macroautophagy; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR023261; Autophagy-related_protein_14.
DR   InterPro; IPR018791; UV_resistance/autophagy_Atg14.
DR   Pfam; PF10186; ATG14; 1.
DR   PRINTS; PR02030; AUTOPHGYRP14.
PE   3: Inferred from homology;
KW   Autophagy; Coiled coil; Membrane; Protein transport; Transport; Vacuole.
FT   CHAIN           1..344
FT                   /note="Autophagy-related protein 14"
FT                   /id="PRO_0000317956"
FT   REGION          3..18
FT                   /note="Cysteine repeats"
FT                   /evidence="ECO:0000250"
FT   COILED          26..156
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   344 AA;  40425 MW;  16764BD9CA2CF536 CRC64;
     MHCPICHHRA HVVYCAHCIN TSPSLLLKLK LDLILLKDEN KELNGKVEQI LNEAMNYDQL
     DIKRMEKKKD PLMNSLMKLD VLRMKKNNNL IRHRIEQLNE RIYSKRNHIS ELKVEIDNYK
     CYKVGTGTDK LIEQVEISDA KNKLAQVSKI CESVRDYKLN LLNNWFVIQK LQDNFQIPFA
     IAFQPLISLK NFRVLPLAIT NDSINIMWKY ISFFSDILMI KLPYTNKICE QPMFEFSDSI
     QTVVQRLIKL IINILQICRH LKLVPSTPMD IPWLLDQYDV DGLFYNMVKR NKMKCRSVSL
     YWTFGMLYSM VLDNMNNPQR GHPARRTAPP PTVTGPHDRW YVVG