ID   PRH1_SCHPO              Reviewed;         719 AA.
AC   Q03319;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   03-AUG-2022, entry version 151.
DE   RecName: Full=Probable ATP-dependent RNA helicase prh1;
DE            EC=3.6.4.13;
GN   Name=prh1; ORFNames=SPAC2G11.11c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1454545; DOI=10.1093/nar/20.21.5841;
RA   Inoue S.B., Sakamoto H., Sawa H., Shimura Y.;
RT   "Nucleotide sequence of a fission yeast gene encoding the DEAH-box RNA
RT   helicase.";
RL   Nucleic Acids Res. 20:5841-5841(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: May be involved in pre-mRNA splicing.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D13249; BAA02516.1; -; Genomic_DNA.
DR   EMBL; CU329670; CAA91176.1; -; Genomic_DNA.
DR   PIR; S35546; S35546.
DR   PIR; S62466; S62466.
DR   RefSeq; NP_593091.1; NM_001018489.2.
DR   AlphaFoldDB; Q03319; -.
DR   SMR; Q03319; -.
DR   BioGRID; 278244; 4.
DR   STRING; 4896.SPAC2G11.11c.1; -.
DR   iPTMnet; Q03319; -.
DR   MaxQB; Q03319; -.
DR   PaxDb; Q03319; -.
DR   PRIDE; Q03319; -.
DR   EnsemblFungi; SPAC2G11.11c.1; SPAC2G11.11c.1:pep; SPAC2G11.11c.
DR   GeneID; 2541750; -.
DR   KEGG; spo:SPAC2G11.11c; -.
DR   PomBase; SPAC2G11.11c; prh1.
DR   VEuPathDB; FungiDB:SPAC2G11.11c; -.
DR   eggNOG; KOG0922; Eukaryota.
DR   HOGENOM; CLU_001832_5_11_1; -.
DR   InParanoid; Q03319; -.
DR   OMA; HIHRTTP; -.
DR   PhylomeDB; Q03319; -.
DR   PRO; PR:Q03319; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005730; C:nucleolus; HDA:PomBase.
DR   GO; GO:1990904; C:ribonucleoprotein complex; IEA:UniProt.
DR   GO; GO:0005524; F:ATP binding; ISM:PomBase.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; ISO:PomBase.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IBA:GO_Central.
DR   GO; GO:0042254; P:ribosome biogenesis; ISO:PomBase.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; mRNA processing; Nucleotide-binding;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..719
FT                   /note="Probable ATP-dependent RNA helicase prh1"
FT                   /id="PRO_0000055155"
FT   DOMAIN          106..269
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          294..466
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          1..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          64..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           216..219
FT                   /note="DEAH box"
FT   BINDING         119..126
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   CONFLICT        719
FT                   /note="T -> HLTFQLLKKIKKAHILSHYILVNLETTIF (in Ref. 1;
FT                   BAA02516)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   719 AA;  80605 MW;  E6EB16EEED852115 CRC64;
     MVKVSGKLRN GGSITKQNLT DGLVGGQRIK APNKKKNRKA SKNTTKKSTV SVVNFLEQDT
     DFGANEVVGS DNSSPEKRSE NSPSKRKDIL EQRKNLPIWE AHDTLCQQIQ DNRVIVVVGE
     TGSGKSTQIP QFLNECPYAQ EGCVAITQPR RVAAVNLAKR VAAEQGCRLG EQVGYSIRFD
     DTTSKKTRIK YLTDGMLLRE LINDPILSQY HTLILDEAHE RTLMTDMLLG FVKKIIKKRP
     ALRVIIMSAT LNAERFSEFF DGAEICYISG RQYPVQIHYT YTPEPDYLDA CLRTIFQLHT
     KLPPGDILVF LTGQDEIEAL EALIKSYSKQ LPSNLPQIQA CPLFASLPQE QQLQVFLPAL
     ANHRKVVLST NIAETSVTIS GIRYVIDTGL AKIKQFNSKL GLESLTVQPI SQSAAMQRSG
     RAGREAAGQC YRIYTEADFD KLPKETIPEI KRIDLSQAVL TLKARGQNDV INFHYMDPPS
     KEGLLRALEH LYSIGALDDN GHINDLGYQM SLIPLLPSLA RAVLAAREHN CLSEVIDVVS
     CLSTDSMFLF PQEKRDEAIE ARLKFLHSEG DLLTCLNALR QYLESSHDSR KQWCSQNFIN
     RRALKTILDI RKQLREHCLK DGWELNSSPE VNSENLLLSF LSGYITNTAL LHPDGSYRTI
     IGNQTISIHP SSSLFGKKVE AIMYHELVFT TKSYVRGVSS IRSNWLNAVA PHYLARRST