ATG14_YEAST
ID ATG14_YEAST Reviewed; 344 AA.
AC P38270; D6VQC5;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Autophagy-related protein 14;
DE AltName: Full=Cytoplasm to vacuole targeting protein 12;
GN Name=ATG14; Synonyms=APG14, CVT12; OrderedLocusNames=YBR128C;
GN ORFNames=YBR1003;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091856; DOI=10.1002/yea.320100002;
RA Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F.,
RA Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M.,
RA Herbert C.J.;
RT "The sequence of 29.7 kb from the right arm of chromosome II reveals 13
RT complete open reading frames, of which ten correspond to new genes.";
RL Yeast 10:S1-S11(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP INTERACTION WITH VPS30.
RX PubMed=9712845; DOI=10.1074/jbc.273.35.22284;
RA Kametaka S., Okano T., Ohsumi M., Ohsumi Y.;
RT "Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in
RT the yeast, Saccharomyces cerevisiae.";
RL J. Biol. Chem. 273:22284-22291(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-344.
RX PubMed=2521486; DOI=10.1016/s0021-9258(18)94254-7;
RA Nelson H., Mandiyan S., Nelson N.;
RT "A conserved gene encoding the 57-kDa subunit of the yeast vacuolar H+-
RT ATPase.";
RL J. Biol. Chem. 264:1775-1778(1989).
RN [7]
RP FUNCTION.
RX PubMed=8224160; DOI=10.1016/0014-5793(93)80398-e;
RA Tsukada M., Ohsumi Y.;
RT "Isolation and characterization of autophagy-defective mutants of
RT Saccharomyces cerevisiae.";
RL FEBS Lett. 333:169-174(1993).
RN [8]
RP FUNCTION.
RX PubMed=8663607; DOI=10.1074/jbc.271.30.17621;
RA Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J.;
RT "Genetic and phenotypic overlap between autophagy and the cytoplasm to
RT vacuole protein targeting pathway.";
RL J. Biol. Chem. 271:17621-17624(1996).
RN [9]
RP FUNCTION.
RX PubMed=10547367; DOI=10.1242/jcs.112.22.4079;
RA Hutchins M.U., Veenhuis M., Klionsky D.J.;
RT "Peroxisome degradation in Saccharomyces cerevisiae is dependent on
RT machinery of macroautophagy and the Cvt pathway.";
RL J. Cell Sci. 112:4079-4087(1999).
RN [10]
RP FUNCTION.
RX PubMed=11689437; DOI=10.1093/emboj/20.21.5971;
RA Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.;
RT "The pre-autophagosomal structure organized by concerted functions of APG
RT genes is essential for autophagosome formation.";
RL EMBO J. 20:5971-5981(2001).
RN [11]
RP INDUCTION.
RX PubMed=11096087; DOI=10.1074/jbc.m008162200;
RA Chan T.F., Bertram P.G., Ai W., Zheng X.F.;
RT "Regulation of APG14 expression by the GATA-type transcription factor
RT Gln3p.";
RL J. Biol. Chem. 276:6463-6467(2001).
RN [12]
RP FUNCTION.
RX PubMed=11382761; DOI=10.1074/jbc.m102346200;
RA Shintani T., Suzuki K., Kamada Y., Noda T., Ohsumi Y.;
RT "Apg2p functions in autophagosome formation on the perivacuolar
RT structure.";
RL J. Biol. Chem. 276:30452-30460(2001).
RN [13]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH VPS15; VPS30 AND
RP VPS34.
RX PubMed=11157979; DOI=10.1083/jcb.152.3.519;
RA Kihara A., Noda T., Ishihara N., Ohsumi Y.;
RT "Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in
RT autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae.";
RL J. Cell Biol. 152:519-530(2001).
RN [14]
RP FUNCTION OF THE VPS34 PI3-KINASE COMPLEX I.
RX PubMed=15004240; DOI=10.1091/mbc.e03-07-0479;
RA Reggiori F., Wang C.W., Nair U., Shintani T., Abeliovich H., Klionsky D.J.;
RT "Early stages of the secretory pathway, but not endosomes, are required for
RT Cvt vesicle and autophagosome assembly in Saccharomyces cerevisiae.";
RL Mol. Biol. Cell 15:2189-2204(2004).
RN [15]
RP FUNCTION.
RX PubMed=16565503; DOI=10.2353/ajpath.2006.051097;
RA Kruse K.B., Dear A., Kaltenbrun E.R., Crum B.E., George P.M., Brennan S.O.,
RA McCracken A.A.;
RT "Mutant fibrinogen cleared from the endoplasmic reticulum via endoplasmic
RT reticulum-associated protein degradation and autophagy: an explanation for
RT liver disease.";
RL Am. J. Pathol. 168:1299-1308(2006).
RN [16]
RP FUNCTION.
RX PubMed=16267277; DOI=10.1091/mbc.e04-09-0779;
RA Kruse K.B., Brodsky J.L., McCracken A.A.;
RT "Characterization of an ERAD gene as VPS30/ATG6 reveals two alternative and
RT functionally distinct protein quality control pathways: one for soluble Z
RT variant of human alpha-1 proteinase inhibitor (A1PiZ) and another for
RT aggregates of A1PiZ.";
RL Mol. Biol. Cell 17:203-212(2006).
RN [17]
RP FUNCTION, DOMAIN, INTERACTION WITH VPS30 AND VPS34, AND SUBCELLULAR
RP LOCATION.
RX PubMed=16421251; DOI=10.1091/mbc.e05-09-0841;
RA Obara K., Sekito T., Ohsumi Y.;
RT "Assortment of phosphatidylinositol 3-kinase complexes--Atg14p directs
RT association of complex I to the pre-autophagosomal structure in
RT Saccharomyces cerevisiae.";
RL Mol. Biol. Cell 17:1527-1539(2006).
RN [18]
RP SUBCELLULAR LOCATION.
RX PubMed=18829864; DOI=10.1091/mbc.e08-05-0544;
RA He C., Baba M., Cao Y., Klionsky D.J.;
RT "Self-interaction is critical for Atg9 transport and function at the
RT phagophore assembly site during autophagy.";
RL Mol. Biol. Cell 19:5506-5516(2008).
RN [19]
RP SUBCELLULAR LOCATION.
RX PubMed=18077553; DOI=10.1091/mbc.e07-08-0826;
RA Cheong H., Nair U., Geng J., Klionsky D.J.;
RT "The Atg1 kinase complex is involved in the regulation of protein
RT recruitment to initiate sequestering vesicle formation for nonspecific
RT autophagy in Saccharomyces cerevisiae.";
RL Mol. Biol. Cell 19:668-681(2008).
RN [20]
RP FUNCTION, AND INTERACTION WITH VPS15.
RX PubMed=19445518; DOI=10.1021/bi900621w;
RA Heenan E.J., Vanhooke J.L., Temple B.R., Betts L., Sondek J.E.,
RA Dohlman H.G.;
RT "Structure and function of Vps15 in the endosomal G protein signaling
RT pathway.";
RL Biochemistry 48:6390-6401(2009).
RN [21]
RP FUNCTION.
RX PubMed=20855505; DOI=10.1083/jcb.200912089;
RA Mari M., Griffith J., Rieter E., Krishnappa L., Klionsky D.J., Reggiori F.;
RT "An Atg9-containing compartment that functions in the early steps of
RT autophagosome biogenesis.";
RL J. Cell Biol. 190:1005-1022(2010).
RN [22]
RP FUNCTION.
RX PubMed=21703229; DOI=10.1016/j.bbrc.2011.06.061;
RA Yamagata M., Obara K., Kihara A.;
RT "Sphingolipid synthesis is involved in autophagy in Saccharomyces
RT cerevisiae.";
RL Biochem. Biophys. Res. Commun. 410:786-791(2011).
RN [23]
RP FUNCTION.
RX PubMed=22718752; DOI=10.1074/jbc.m112.351825;
RA Baek G.H., Cheng H., Kim I., Rao H.;
RT "The Cdc48 protein and its cofactor Vms1 are involved in Cdc13 protein
RT degradation.";
RL J. Biol. Chem. 287:26788-26795(2012).
RN [24]
RP SUBCELLULAR LOCATION.
RX PubMed=23129774; DOI=10.1074/jbc.m112.411454;
RA Kakuta S., Yamamoto H., Negishi L., Kondo-Kakuta C., Hayashi N., Ohsumi Y.;
RT "Atg9 vesicles recruit vesicle-tethering proteins Trs85 and Ypt1 to the
RT autophagosome formation site.";
RL J. Biol. Chem. 287:44261-44269(2012).
RN [25]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE
RP AUTOPHAGY-SPECIFIC VPS34 PI3-KINASE COMPLEX, AND INTERACTION WITH ARG38.
RX PubMed=24165940; DOI=10.1083/jcb.201304123;
RA Araki Y., Ku W.C., Akioka M., May A.I., Hayashi Y., Arisaka F.,
RA Ishihama Y., Ohsumi Y.;
RT "Atg38 is required for autophagy-specific phosphatidylinositol 3-kinase
RT complex integrity.";
RL J. Cell Biol. 203:299-313(2013).
CC -!- FUNCTION: Required for cytoplasm to vacuole transport (Cvt) and
CC autophagy as a part of the autophagy-specific VPS34 PI3-kinase complex
CC I. This complex is essential to recruit the ATG8-phosphatidylinositol
CC conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal
CC structure. ATG14 mediates the specific binding of the VPS34 PI3-kinase
CC complex I to the preautophagosomal structure (PAS).
CC {ECO:0000269|PubMed:10547367, ECO:0000269|PubMed:11157979,
CC ECO:0000269|PubMed:11382761, ECO:0000269|PubMed:11689437,
CC ECO:0000269|PubMed:15004240, ECO:0000269|PubMed:16267277,
CC ECO:0000269|PubMed:16421251, ECO:0000269|PubMed:16565503,
CC ECO:0000269|PubMed:19445518, ECO:0000269|PubMed:20855505,
CC ECO:0000269|PubMed:21703229, ECO:0000269|PubMed:22718752,
CC ECO:0000269|PubMed:8224160, ECO:0000269|PubMed:8663607,
CC ECO:0000269|PubMed:9712845}.
CC -!- SUBUNIT: Component of the autophagy-specific VPS34 PI3-kinase complex I
CC composed of VPS15, VPS30, VPS34, ATG14 and ATG38. Interacts directly
CC with ATG38. {ECO:0000269|PubMed:11157979, ECO:0000269|PubMed:16421251,
CC ECO:0000269|PubMed:19445518, ECO:0000269|PubMed:24165940,
CC ECO:0000269|PubMed:9712845}.
CC -!- INTERACTION:
CC P38270; Q05789: ATG38; NbExp=15; IntAct=EBI-2699, EBI-35873;
CC P38270; Q02948: VPS30; NbExp=12; IntAct=EBI-2699, EBI-2670;
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane; Peripheral
CC membrane protein. Vacuole membrane; Peripheral membrane protein.
CC Note=TRS85 is required for the recruitment of ATG14 to the PAS.
CC -!- INDUCTION: Nitrogen starvation or rapamycin treatment rapidly causes a
CC more than 20-fold induction of expression. The expression is dependent
CC on GLN3. {ECO:0000269|PubMed:11096087}.
CC -!- DOMAIN: Coiled-Coils at the N-terminal half are essential for
CC interaction with VPS30 and VPS34 and autophagy.
CC {ECO:0000269|PubMed:16421251}.
CC -!- SIMILARITY: Belongs to the ATG14 family. {ECO:0000305}.
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DR EMBL; X75891; CAA53487.1; -; Genomic_DNA.
DR EMBL; Z35997; CAA85085.1; -; Genomic_DNA.
DR EMBL; AB011071; BAA32103.1; -; Genomic_DNA.
DR EMBL; AY693167; AAT93186.1; -; Genomic_DNA.
DR EMBL; J04450; AAA66889.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07245.1; -; Genomic_DNA.
DR PIR; S45997; S45997.
DR RefSeq; NP_009686.1; NM_001178476.1.
DR AlphaFoldDB; P38270; -.
DR SMR; P38270; -.
DR BioGRID; 32829; 163.
DR ComplexPortal; CPX-1881; Phosphatidylinositol 3-kinase complex, class III, type I.
DR DIP; DIP-2741N; -.
DR IntAct; P38270; 8.
DR MINT; P38270; -.
DR STRING; 4932.YBR128C; -.
DR PaxDb; P38270; -.
DR PRIDE; P38270; -.
DR EnsemblFungi; YBR128C_mRNA; YBR128C; YBR128C.
DR GeneID; 852425; -.
DR KEGG; sce:YBR128C; -.
DR SGD; S000000332; ATG14.
DR VEuPathDB; FungiDB:YBR128C; -.
DR eggNOG; ENOG502RY86; Eukaryota.
DR HOGENOM; CLU_069448_0_0_1; -.
DR InParanoid; P38270; -.
DR OMA; MYCSHCI; -.
DR BioCyc; YEAST:G3O-29083-MON; -.
DR PRO; PR:P38270; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38270; protein.
DR GO; GO:0019898; C:extrinsic component of membrane; IDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
DR GO; GO:0000407; C:phagophore assembly site; IDA:SGD.
DR GO; GO:0034045; C:phagophore assembly site membrane; IC:ComplexPortal.
DR GO; GO:0034271; C:phosphatidylinositol 3-kinase complex, class III, type I; IDA:SGD.
DR GO; GO:0120095; C:vacuole-isolation membrane contact site; IDA:SGD.
DR GO; GO:0006914; P:autophagy; IDA:ComplexPortal.
DR GO; GO:0030242; P:autophagy of peroxisome; IMP:SGD.
DR GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IMP:SGD.
DR GO; GO:0016236; P:macroautophagy; IMP:SGD.
DR GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; IC:ComplexPortal.
DR GO; GO:0034727; P:piecemeal microautophagy of the nucleus; IMP:SGD.
DR InterPro; IPR023261; Autophagy-related_protein_14.
DR InterPro; IPR018791; UV_resistance/autophagy_Atg14.
DR Pfam; PF10186; ATG14; 1.
DR PRINTS; PR02030; AUTOPHGYRP14.
PE 1: Evidence at protein level;
KW Autophagy; Coiled coil; Membrane; Protein transport; Reference proteome;
KW Transport; Vacuole.
FT CHAIN 1..344
FT /note="Autophagy-related protein 14"
FT /id="PRO_0000212453"
FT REGION 3..18
FT /note="Cysteine repeats"
FT /evidence="ECO:0000250"
FT COILED 25..156
FT /evidence="ECO:0000255"
SQ SEQUENCE 344 AA; 40454 MW; 202039BC7EC8BB16 CRC64;
MHCPICHHRA HVVYCAHCIN TSPSLLLKLK LDLILLKDEN KELNGKVEQI LNEAMNYDQL
DIKRMEKKKD PLMNSLMKLD VLRMKKNNNL IRHRIEQLNE RIYSKRNHIS ELKVEIDNYK
CYKVGTGTDK LREQVEISDA KNKLAQVSKI CESARDYKLN LLNNWFVIQK LQDNFQIPFA
IAFQPLISLK NFRILPLAIT NDSINIMWKY ISFFSDILMI KLPYTNKICE QPMFEFSDSI
QTVVQRLIKL IINILQICRH LKLVPSTPMD IPWLLDQYDV DGLFYNMVKR NKMKCRSVSL
YWTFGMLYSM VLDNMNNPQR GHPARRTAPP PTVTGPHDRW YVVG
