ID   PRI1_CAEEL              Reviewed;         410 AA.
AC   P34471;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=DNA primase small subunit;
DE            EC=2.7.7.-;
GN   Name=pri-1; ORFNames=F58A4.4;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=7906398; DOI=10.1038/368032a0;
RA   Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA   Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA   Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA   Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA   Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA   Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA   Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA   Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA   Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA   Wilkinson-Sproat J., Wohldman P.;
RT   "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT   elegans.";
RL   Nature 368:32-38(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: DNA primase is the polymerase that synthesizes small RNA
CC       primers for the Okazaki fragments made during discontinuous DNA
CC       replication. {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of a small subunit and a large subunit.
CC   -!- MISCELLANEOUS: The bound zinc ion is not a cofactor. It is bound to a
CC       zinc knuckle motif that may be involved in sequence recognition and the
CC       binding of ssDNA (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the eukaryotic-type primase small subunit
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA80161.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; Z22179; CAA80161.1; ALT_INIT; Genomic_DNA.
DR   PIR; S40976; S40976.
DR   RefSeq; NP_001255015.1; NM_001268086.1.
DR   AlphaFoldDB; P34471; -.
DR   SMR; P34471; -.
DR   BioGRID; 41553; 3.
DR   IntAct; P34471; 2.
DR   MINT; P34471; -.
DR   STRING; 6239.F58A4.4a; -.
DR   EPD; P34471; -.
DR   PaxDb; P34471; -.
DR   PeptideAtlas; P34471; -.
DR   EnsemblMetazoa; F58A4.4a.1; F58A4.4a.1; WBGene00004180.
DR   GeneID; 176358; -.
DR   KEGG; cel:CELE_F58A4.4; -.
DR   UCSC; F58A4.4; c. elegans.
DR   CTD; 176358; -.
DR   WormBase; F58A4.4a; CE00220; WBGene00004180; pri-1.
DR   eggNOG; KOG2851; Eukaryota.
DR   GeneTree; ENSGT00390000011466; -.
DR   HOGENOM; CLU_028288_3_2_1; -.
DR   InParanoid; P34471; -.
DR   OMA; NVTRGFN; -.
DR   OrthoDB; 1346165at2759; -.
DR   PhylomeDB; P34471; -.
DR   Reactome; R-CEL-113501; Inhibition of replication initiation of damaged DNA by RB1/E2F1.
DR   Reactome; R-CEL-68952; DNA replication initiation.
DR   Reactome; R-CEL-68962; Activation of the pre-replicative complex.
DR   Reactome; R-CEL-69091; Polymerase switching.
DR   Reactome; R-CEL-69166; Removal of the Flap Intermediate.
DR   Reactome; R-CEL-69183; Processive synthesis on the lagging strand.
DR   PRO; PR:P34471; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00004180; Expressed in germ line (C elegans) and 4 other tissues.
DR   ExpressionAtlas; P34471; baseline and differential.
DR   GO; GO:0005658; C:alpha DNA polymerase:primase complex; IBA:GO_Central.
DR   GO; GO:0003896; F:DNA primase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IBA:GO_Central.
DR   CDD; cd04860; AE_Prim_S; 1.
DR   InterPro; IPR002755; DNA_primase_S.
DR   InterPro; IPR014052; DNA_primase_ssu_euk/arc.
DR   PANTHER; PTHR10536; PTHR10536; 1.
DR   Pfam; PF01896; DNA_primase_S; 1.
DR   TIGRFAMs; TIGR00335; primase_sml; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Primosome; Reference proteome; Transcription;
KW   Transferase; Zinc.
FT   CHAIN           1..410
FT                   /note="DNA primase small subunit"
FT                   /id="PRO_0000046732"
FT   MOTIF           118..129
FT                   /note="Zinc knuckle motif"
FT   ACT_SITE        43
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        106
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        108
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   410 AA;  48097 MW;  833C4EE597D527D6 CRC64;
     MSYNSIRLPQ DLPIYYKNFF PVKPFTKWLR YGQNNGDYFN RREFAFILAD DVHIRYRSYN
     DEHAFFKALS STNPEKLDIG AVYNHEPINN KRHTDYQAVE RELVFDIDLT DYDPVRNCCK
     DATVCPKCWK FMVLAVKILD FQLEDMFDFK ARMWVFSGRR GVHCWVGDKK ARMLNNYQRS
     AIATRLNLFK KNGQCEVTEG RAKMTKVPPI VRDAFNVALK DGVFEKMIYD QGWLDKEDFI
     TEYKFMSEVG RDDLRSLSET YKTPQERWNM IRGLFDDDYR KSLSPKDGLL DFKMQGRDRN
     YLLYFVLQRC YPRLDVNVST GTNHLLKSPF CIHPKTGNVA VPLNVGKIEE FDVSKCPRID
     HVVEELSSLL AERGNDENED SKNRKFLAYK HGALAPYVEN FEKFVSACIS