PRI2_ARATH
ID PRI2_ARATH Reviewed; 454 AA.
AC Q84WJ2; Q0WRZ1; Q8LFN6; Q9FYG1;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Probable DNA primase large subunit;
GN OrderedLocusNames=At1g67320; ORFNames=F1N21_14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA primase is the polymerase that synthesizes small RNA
CC primers for the Okazaki fragments made during discontinuous DNA
CC replication. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC -!- SUBUNIT: Heterodimer of a small subunit and a large subunit.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q84WJ2-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the eukaryotic-type primase large subunit
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG00249.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002130; AAG00249.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34629.1; -; Genomic_DNA.
DR EMBL; BT003155; AAO24587.1; -; mRNA.
DR EMBL; AK228152; BAF00108.1; -; mRNA.
DR EMBL; AY084736; AAM61309.1; -; mRNA.
DR PIR; G96696; G96696.
DR RefSeq; NP_001185340.1; NM_001198411.2. [Q84WJ2-1]
DR AlphaFoldDB; Q84WJ2; -.
DR SMR; Q84WJ2; -.
DR BioGRID; 28273; 2.
DR STRING; 3702.AT1G67320.3; -.
DR PRIDE; Q84WJ2; -.
DR DNASU; 843052; -.
DR EnsemblPlants; AT1G67320.2; AT1G67320.2; AT1G67320. [Q84WJ2-1]
DR GeneID; 843052; -.
DR Gramene; AT1G67320.2; AT1G67320.2; AT1G67320. [Q84WJ2-1]
DR KEGG; ath:AT1G67320; -.
DR Araport; AT1G67320; -.
DR eggNOG; KOG2267; Eukaryota.
DR HOGENOM; CLU_026253_2_1_1; -.
DR InParanoid; Q84WJ2; -.
DR OMA; KTSMPLC; -.
DR PhylomeDB; Q84WJ2; -.
DR PRO; PR:Q84WJ2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84WJ2; baseline and differential.
DR Genevisible; Q84WJ2; AT.
DR GO; GO:0005658; C:alpha DNA polymerase:primase complex; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006270; P:DNA replication initiation; IEA:UniProt.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR CDD; cd07322; PriL_PriS_Eukaryotic; 1.
DR InterPro; IPR016558; DNA_primase_lsu_euk.
DR InterPro; IPR007238; DNA_primase_lsu_euk/arc.
DR PANTHER; PTHR10537; PTHR10537; 1.
DR Pfam; PF04104; DNA_primase_lrg; 1.
DR PIRSF; PIRSF009449; DNA_primase_large_subunit; 1.
PE 2: Evidence at transcript level;
KW 4Fe-4S; Alternative splicing; DNA replication; DNA-binding; Iron;
KW Iron-sulfur; Metal-binding; Primosome; Reference proteome.
FT CHAIN 1..454
FT /note="Probable DNA primase large subunit"
FT /id="PRO_0000046773"
FT BINDING 280
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 359
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 375
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 415
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT CONFLICT 18
FT /note="T -> A (in Ref. 3; AAO24587 and 4; BAF00108)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 454 AA; 52074 MW; A6DCA1913EA7D9AE CRC64;
MEVIRSQKRT VSNDVVSTPT LPLYLTAPHM EVRLEEFELF AIDRLRVLKG VSDGLARARN
PNEMDDLVET LWKEHMRLSN VSEMINKDII SHFVLRLVYC RSDELKKWFL SMETALFRHR
FRLKKIEEQR AIVGEFGLPY KAVIGAELES LKERLGLVAR SHGQISSDVE NIYYKVPFEE
VPDLVASRRV LLQKGFAFVA GTQLVSLVVT QFRSHLSKAL ILTNRKWTTT IREREKDRLT
PIVEALSTSY LGPDYSQSNE YADISLKDID QVSKSSFPLC MRHLFEKLRE DHHLKHGGRM
QLGLFLKGVG LKLDDALAFW REEFTKKVGS ERFDKEYAYA IRHNYGKEGK RTDYTPYACS
KIITSAPGAG DHHGCPYRHF SEDNLKAALS RMGLSSRGME DVMDKVRNRH YQLACTLTFE
AVYGTSCDTG INHPNQYFEE SQKILKSKTP AAPV
