ID   PRI2_CAEEL              Reviewed;         503 AA.
AC   O02334;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2001, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=DNA primase large subunit;
GN   Name=pri-2; ORFNames=W02D9.1;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: DNA primase is the polymerase that synthesizes small RNA
CC       primers for the Okazaki fragments made during discontinuous DNA
CC       replication. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- SUBUNIT: Heterodimer of a small subunit and a large subunit.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the eukaryotic-type primase large subunit
CC       family. {ECO:0000305}.
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DR   EMBL; Z81137; CAB03469.2; -; Genomic_DNA.
DR   PIR; G87948; G87948.
DR   PIR; T26114; T26114.
DR   RefSeq; NP_001251922.1; NM_001264993.1.
DR   AlphaFoldDB; O02334; -.
DR   SMR; O02334; -.
DR   BioGRID; 38519; 1.
DR   STRING; 6239.W02D9.1a.1; -.
DR   EPD; O02334; -.
DR   PaxDb; O02334; -.
DR   PeptideAtlas; O02334; -.
DR   PRIDE; O02334; -.
DR   EnsemblMetazoa; W02D9.1a.1; W02D9.1a.1; WBGene00004181.
DR   GeneID; 173122; -.
DR   KEGG; cel:CELE_W02D9.1; -.
DR   UCSC; W02D9.1.1; c. elegans.
DR   CTD; 173122; -.
DR   WormBase; W02D9.1a; CE24028; WBGene00004181; pri-2.
DR   eggNOG; KOG2267; Eukaryota.
DR   GeneTree; ENSGT00390000009790; -.
DR   HOGENOM; CLU_026253_2_0_1; -.
DR   InParanoid; O02334; -.
DR   OMA; KTSMPLC; -.
DR   OrthoDB; 479347at2759; -.
DR   PhylomeDB; O02334; -.
DR   Reactome; R-CEL-113501; Inhibition of replication initiation of damaged DNA by RB1/E2F1.
DR   Reactome; R-CEL-68952; DNA replication initiation.
DR   Reactome; R-CEL-68962; Activation of the pre-replicative complex.
DR   Reactome; R-CEL-69091; Polymerase switching.
DR   Reactome; R-CEL-69166; Removal of the Flap Intermediate.
DR   Reactome; R-CEL-69183; Processive synthesis on the lagging strand.
DR   PRO; PR:O02334; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00004181; Expressed in germ line (C elegans) and 4 other tissues.
DR   ExpressionAtlas; O02334; baseline and differential.
DR   GO; GO:0005658; C:alpha DNA polymerase:primase complex; IBA:GO_Central.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:UniProt.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   CDD; cd07322; PriL_PriS_Eukaryotic; 1.
DR   InterPro; IPR016558; DNA_primase_lsu_euk.
DR   InterPro; IPR007238; DNA_primase_lsu_euk/arc.
DR   PANTHER; PTHR10537; PTHR10537; 1.
DR   Pfam; PF04104; DNA_primase_lrg; 1.
DR   PIRSF; PIRSF009449; DNA_primase_large_subunit; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; DNA replication; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW   Primosome; Reference proteome.
FT   CHAIN           1..503
FT                   /note="DNA primase large subunit"
FT                   /id="PRO_0000046771"
FT   BINDING         309
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         388
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         405
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         446
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   503 AA;  58274 MW;  1207C009637376EF CRC64;
     MIFTSGANKL NDNRNVRTKI SSNRQSIKTA PTKAAEIPEY LQLYQTPPGD DISLTEFDDI
     AMERLKLLKS FENLKDSVSL YNDEFKDKFR KLCDGTKLIV FPDQKPPHQE EVYELWRRDN
     IGHFILRLAF CRTPENQKWL TQIEGDFLRF RLRQEREQVL DSALANVNFT IEKLGYTEKQ
     AMIEDLEAAC QFNLFEAPNK TFYKVDFIDA IELIRRGSVH LKKGFAYFPF DDLVTILVTK
     MKNNMMAAMA RSFKHLAILE EEGRLLPRLS LLSNNAYSGK DYNGEQPDGS IIVTRHMIDK
     LSRRSFAPCM KQMHNHLRVN HHLKYGARRQ YGLFLKGIGL SLDEAIAMMR DEFTKKITSD
     KFDKEYGYNI RYMYGKEGRR VAQTAMSCAT IILRNPPSAV DCHGCPFRHS EKQVLKQKLG
     SDAILKKEQI ERIAELAELN QYDKACTRYF EFMHNLEEGG LGQLITHPNQ FYEESQKIVA
     ERIAAKQESQ EAPAPKIEKM DTE