ID   PRI2_NEUCR              Reviewed;         513 AA.
AC   Q8NIZ4; Q7S3B8;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Probable DNA primase large subunit;
GN   ORFNames=5F3.150, NCU04646;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: DNA primase is the polymerase that synthesizes small RNA
CC       primers for the Okazaki fragments made during discontinuous DNA
CC       replication. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. {ECO:0000250};
CC   -!- SUBUNIT: Heterodimer of a small subunit and a large subunit.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the eukaryotic-type primase large subunit
CC       family. {ECO:0000305}.
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DR   EMBL; AL807371; CAD37016.1; -; Genomic_DNA.
DR   EMBL; CM002240; EAA29953.1; -; Genomic_DNA.
DR   RefSeq; XP_959189.1; XM_954096.2.
DR   AlphaFoldDB; Q8NIZ4; -.
DR   SMR; Q8NIZ4; -.
DR   STRING; 5141.EFNCRP00000005897; -.
DR   EnsemblFungi; EAA29953; EAA29953; NCU04646.
DR   GeneID; 3875327; -.
DR   KEGG; ncr:NCU04646; -.
DR   VEuPathDB; FungiDB:NCU04646; -.
DR   HOGENOM; CLU_026253_1_0_1; -.
DR   InParanoid; Q8NIZ4; -.
DR   OMA; KTSMPLC; -.
DR   Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR   GO; GO:0005658; C:alpha DNA polymerase:primase complex; IBA:GO_Central.
DR   GO; GO:0005635; C:nuclear envelope; IEA:EnsemblFungi.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:EnsemblFungi.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:EnsemblFungi.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:EnsemblFungi.
DR   GO; GO:0006302; P:double-strand break repair; IEA:EnsemblFungi.
DR   CDD; cd07322; PriL_PriS_Eukaryotic; 1.
DR   InterPro; IPR016558; DNA_primase_lsu_euk.
DR   InterPro; IPR007238; DNA_primase_lsu_euk/arc.
DR   PANTHER; PTHR10537; PTHR10537; 1.
DR   Pfam; PF04104; DNA_primase_lrg; 1.
DR   PIRSF; PIRSF009449; DNA_primase_large_subunit; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; DNA replication; DNA-binding; Iron; Iron-sulfur; Metal-binding;
KW   Primosome; Reference proteome.
FT   CHAIN           1..513
FT                   /note="Probable DNA primase large subunit"
FT                   /id="PRO_0000046774"
FT   BINDING         315
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         398
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         415
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         457
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   513 AA;  59522 MW;  64D1921AF0419032 CRC64;
     MLRSNVSRID PKRRNVLDHR KKQFAEAAYK DTDYPHRLNF YSTPPTADIT IEQFEQWAID
     RLRILAELEA CSFRNKTPAE TASHMKPLLD KYLPLDTNSS SSSQLFAQRQ KDHYSHFILR
     LAFASTEDLR RRFTRVETML FRMRLNADDG RERAAFINSL NLDWETVSDE EKRELAAELA
     ATASFGGYKK GQQQQQQYEE DQQTWCKVSW LRVPELVEQR RVFLRQGYAY VPAREQQAMV
     VSEFSSRLER QLELTARALP RLDEDDRLTP ILAHLSKNFI TPDASYVGTS SAISSADISA
     RNIDTLVNNH HFPACMSHLH RTLRRDAHLK HYGRLQYTLF LKGIGLNLEE CLLFWRQSFN
     KITDDTFNKE YRYNVRHTYG DVGGDSNRRG GGYSPYSCQK ILTEHPPGPG EAHGCPYRHF
     NMENLQTLLQ QGMGVTDRGV LNGVKEDKEK QKFHMACNRV FEHLHKEELK KAKDEGIMTA
     AQLETIVHPN EYFKRSYLLK NMGKMQGDVK MEG