ID   PRIA_BUCAI              Reviewed;         726 AA.
AC   P57220;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983}; OrderedLocusNames=BU120;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB12838.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BA000003; BAB12838.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_239952.2; NC_002528.1.
DR   RefSeq; WP_010895957.1; NC_002528.1.
DR   AlphaFoldDB; P57220; -.
DR   SMR; P57220; -.
DR   STRING; 107806.10038803; -.
DR   EnsemblBacteria; BAB12838; BAB12838; BAB12838.
DR   KEGG; buc:BU120; -.
DR   PATRIC; fig|107806.10.peg.129; -.
DR   eggNOG; COG1198; Bacteria.
DR   HOGENOM; CLU_013353_3_1_6; -.
DR   OMA; RCHYCGY; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1440.60; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   InterPro; IPR041236; PriA_C.
DR   Pfam; PF17764; PriA_3primeBD; 1.
DR   Pfam; PF18074; PriA_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00595; priA; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Primosome; Reference proteome; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..726
FT                   /note="Primosomal protein N'"
FT                   /id="PRO_0000102118"
FT   DOMAIN          210..373
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         431..443
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         458..474
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   MOTIF           316..319
FT                   /note="DEAH box"
SQ   SEQUENCE   726 AA;  85642 MW;  06EAB856DC5C1ABE CRC64;
     MIIVKVILPI PIRQYFTYLM PDFMCPIIGG RILVPFNSKD VIGIVASFYK KNNVDQANFK
     HIKALIDTES LYSNMVLDII SWISNNYHCP AGNLFFSILP KILHSDYIIK NKYICQWSIT
     KKGQELNLNY FKRRKKQLYV LLILKKKHIL STELKKYNIS KIILKKLEIQ ELCKVNLNYK
     ISFKRKIIRT KKKLFFNKKI SIVLNDVLKK QCFSSWLLTR VNLYLKVKFY LGLIQSVLYK
     GVQILILVPY IKNINTIAFF LEKYFNASID VMHSKLTSSK YFSNWVRTKN GENSIVIGTG
     KSIFLPFLKL GLIILLEEHN LKYKSINQCR YNIRDLGILR AYKEKIPIIL DSETPSLKTL
     NNVLHRKCFY IKLNKYNHVN QINNNIINLK TEKIKFGLSL TLINEIYKNF TGKQVLLIFN
     KFVLFFFVLM CQKCNEIFKC TNCDDYFEIN QYRNILFCKF CLIQIKKPIF CYNCGSFSLI
     VFKIGAEEIK KEMHSIFPKI PFFFFLNEKN INKNILNTKS FEFAISSPCI IIVTEELVQN
     YYFPHVKLIS LICIDNYFLS FNYRAMEYFA QFYINLNQLT RSTKKSCKIF IQTSFPNDIN
     LKEICNNGYF SFSKKAMAIR KSFLLPPWSF QTIVYSASTN TKYNIIFLSL MRKILQKKSR
     KYNCFLWVLG PNNAFLSINK HKYFHQLLIQ CSSRVALNNV LNESIDVINI FTISKRVKWF
     IDIEPN