ID   PRIA_CHLMU              Reviewed;         753 AA.
AC   Q9PLE5;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 130.
DE   RecName: Full=Primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983}; OrderedLocusNames=TC_0159;
OS   Chlamydia muridarum (strain MoPn / Nigg).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=243161;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MoPn / Nigg;
RX   PubMed=10684935; DOI=10.1093/nar/28.6.1397;
RA   Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O.,
RA   Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S.,
RA   Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J.,
RA   Gwinn M.L., Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G.,
RA   Salzberg S.L., Eisen J.A., Fraser C.M.;
RT   "Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae
RT   AR39.";
RL   Nucleic Acids Res. 28:1397-1406(2000).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
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DR   EMBL; AE002160; AAF39035.1; -; Genomic_DNA.
DR   PIR; D81734; D81734.
DR   RefSeq; WP_010229552.1; NZ_CP027217.1.
DR   AlphaFoldDB; Q9PLE5; -.
DR   SMR; Q9PLE5; -.
DR   STRING; 243161.TC_0159; -.
DR   EnsemblBacteria; AAF39035; AAF39035; TC_0159.
DR   GeneID; 1245693; -.
DR   KEGG; cmu:TC_0159; -.
DR   eggNOG; COG1198; Bacteria.
DR   HOGENOM; CLU_013353_3_1_0; -.
DR   OMA; RCHYCGY; -.
DR   OrthoDB; 1132322at2; -.
DR   Proteomes; UP000000800; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1440.60; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   InterPro; IPR041236; PriA_C.
DR   InterPro; IPR040498; PriA_CRR.
DR   Pfam; PF17764; PriA_3primeBD; 1.
DR   Pfam; PF18074; PriA_C; 1.
DR   Pfam; PF18319; PriA_CRR; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00595; priA; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Primosome; Zinc; Zinc-finger.
FT   CHAIN           1..753
FT                   /note="Primosomal protein N'"
FT                   /id="PRO_0000102120"
FT   DOMAIN          228..395
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   DOMAIN          491..646
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         458..470
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         485..502
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   MOTIF           337..340
FT                   /note="DEAH box"
FT   BINDING         241..248
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
SQ   SEQUENCE   753 AA;  84764 MW;  CD4CFEE77FC4AB05 CRC64;
     MDPTHQSFRL YAEVLVSANI NKILDYGIPA ELEDRVTIGS VVKVPLQRKV TNDKYKFAIV
     LKIKDSSDFA NVIQPIAGIS YEGVTLPKDL IDLIFWISQY YFCPLGSTLS LFLPTVYSQT
     HSTKHQNNVF LGQNAERTQE IIKSIEDPHQ IAVLRKLLKT TKPLTPKELI KKTDISTKIL
     DSLSKQGFIR IVDSANLEIQ DEQLQYFLPE PPTLTQEQIE AINTISQSLI TTKFQTCLLF
     GVTGSGKTEV YLQVIRKARS LGKSVILLVP EVALTIQTLS FFKMHFGAEV GVLHYKLSES
     ERTQTWHKAA RGLINIIIGP RSAIFCPMQN LGLIIVDEEH DGAYKQSDLP PFYQARDVAV
     MRGKFTNATV ILGSATPSLE SYTNALSKKY TLSVLSKRAS TSIPTKVSLI DMNREMEKTR
     KKILFSPTVI RSIEQRLAVG EQTIIFFNRR GFHTNVSCSS CKYTLKCPHC DMILTFHKTE
     RILLCHLCNT RLSKPITSCP QCHGTMTLQY RGAGTEKIEA LLQDFFPTVR TIRLDSDTTR
     YRGSHDALIK QFATGKADIL IGTQMIAKGM HFPAVTLSVV LSGDSGLYIP DFRAAEQVFQ
     LITQVTGRSG RSYLPGEVLI QTFLPQNSTI SHALAQDFPA FYKEEILGRK ACNYPPFTRL
     IRCIFLGKCA EYTLQEAQRM HELIKQNLDA QASLMDISPC GHFKVKDLFH YQFLIKTRNI
     LTVNKQLQDA FSAAKLSSKV RCIVDIDPIT TFF