PRIA_CHLTR
ID PRIA_CHLTR Reviewed; 753 AA.
AC O84783;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN Name=priA {ECO:0000255|HAMAP-Rule:MF_00983}; OrderedLocusNames=CT_778;
OS Chlamydia trachomatis (strain D/UW-3/Cx).
OC Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC Chlamydia/Chlamydophila group; Chlamydia.
OX NCBI_TaxID=272561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=D/UW-3/Cx;
RX PubMed=9784136; DOI=10.1126/science.282.5389.754;
RA Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L.,
RA Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.;
RT "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia
RT trachomatis.";
RL Science 282:754-759(1998).
CC -!- FUNCTION: Involved in the restart of stalled replication forks.
CC Recognizes and binds the arrested nascent DNA chain at stalled
CC replication forks. It can open the DNA duplex, via its helicase
CC activity, and promote assembly of the primosome and loading of the
CC major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC Rule:MF_00983}.
CC -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00983}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE001273; AAC68373.1; -; Genomic_DNA.
DR PIR; B71472; B71472.
DR RefSeq; NP_220297.1; NC_000117.1.
DR RefSeq; WP_010725341.1; NC_000117.1.
DR AlphaFoldDB; O84783; -.
DR SMR; O84783; -.
DR STRING; 813.O172_04335; -.
DR PRIDE; O84783; -.
DR EnsemblBacteria; AAC68373; AAC68373; CT_778.
DR GeneID; 884574; -.
DR KEGG; ctr:CT_778; -.
DR PATRIC; fig|272561.5.peg.854; -.
DR HOGENOM; CLU_013353_3_1_0; -.
DR InParanoid; O84783; -.
DR OMA; RCHYCGY; -.
DR Proteomes; UP000000431; Chromosome.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR Gene3D; 3.40.1440.60; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00983; PriA; 1.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005259; PriA.
DR InterPro; IPR041222; PriA_3primeBD.
DR InterPro; IPR042115; PriA_3primeBD_sf.
DR InterPro; IPR041236; PriA_C.
DR InterPro; IPR040498; PriA_CRR.
DR Pfam; PF17764; PriA_3primeBD; 1.
DR Pfam; PF18074; PriA_C; 1.
DR Pfam; PF18319; PriA_CRR; 1.
DR Pfam; PF04851; ResIII; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00595; priA; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW Metal-binding; Nucleotide-binding; Primosome; Reference proteome; Zinc;
KW Zinc-finger.
FT CHAIN 1..753
FT /note="Primosomal protein N'"
FT /id="PRO_0000102122"
FT DOMAIN 228..395
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT DOMAIN 491..646
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT ZN_FING 458..470
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT ZN_FING 485..502
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT MOTIF 337..340
FT /note="DEAH box"
FT BINDING 241..248
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
SQ SEQUENCE 753 AA; 84832 MW; C91861B4385C5E35 CRC64;
MDPTHQPFRL YAEVIVNANI NKILDYGIPA ELENLVTVGS VVKVPLQRKL TNDKYKIAIV
LKIKSSSDFV HVIQPILDIS YEGITLPQDL IDLIFWISQY YFCPLGSAVS LFLPTVYAQT
HSTKHQNNVF LGQNAERTQE ILKTLDNPQQ IAVLRKLLKT TKPLTPPELM RKTEVSAKTL
DALVKQKFIR IVDSADLEIQ DEQLHYFLPD PPTLNQEQLD AVNTISQSLV AEQFQTCLLF
GVTGSGKTEV YLQVIRKARA LGKSVILLVP EVALTIQTLS FFKMHFGSEV GVLHYKLSDS
ERTQTWYKAS RGLINIIIGP RSAIFCPIQN LGLIIVDEEH DSAYKQSDLP PFYQARDVAV
MRGKMTNATV ILGSATPSLE SYTNALSKKY TLSVLSKRAS TSTPTKVFLI DMNLEMEKTR
KKPFFSQTVI RSIEQRLEVG EQTIIFFNRR GFHTNVSCSS CKYTLKCPHC DMILTFHKTE
RILLCHLCNT RLSKPITSCP QCLGTMTLQY RGAGTEKIET LLREFFPTAR TIRLDSDTTR
FRGSHDALVK QFATGKADIL IGTQMIAKGM HFPAVTLSVV LSGDSGLYIP DFRAAEQVFQ
LITQVTGRSG RSHLPGEVLI QTFLPQNSTI SHALAQDFPA FYKEEILGRK VCNYPPFTRL
IRCIFLGKCS DYTLKETQRV HTLIKQNLDS QASLMEISPC GHFKVKDLFH YQFLIKTRNI
LVANKQIQEA LAAAKLSSKV RCIVDVDPVT TFF
