ID   PRIA_MYCBO              Reviewed;         655 AA.
AC   P0A5A6; A0A1R3XYA0; P71670; X2BI84;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Probable primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   OrderedLocusNames=BQ2027_MB1437;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- CAUTION: Compared to other bacterial PriA, it has a very divergent
CC       helicase domain. {ECO:0000305}.
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DR   EMBL; LT708304; SIU00040.1; -; Genomic_DNA.
DR   RefSeq; NP_855089.1; NC_002945.3.
DR   RefSeq; WP_003407285.1; NC_002945.4.
DR   AlphaFoldDB; P0A5A6; -.
DR   SMR; P0A5A6; -.
DR   EnsemblBacteria; SIU00040; SIU00040; BQ2027_MB1437.
DR   GeneID; 45425380; -.
DR   PATRIC; fig|233413.5.peg.1572; -.
DR   OMA; CTVEAAQ; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1440.60; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   Pfam; PF17764; PriA_3primeBD; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Primosome; Zinc; Zinc-finger.
FT   CHAIN           1..655
FT                   /note="Probable primosomal protein N'"
FT                   /id="PRO_0000102127"
FT   ZN_FING         368..380
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         396..411
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
SQ   SEQUENCE   655 AA;  69839 MW;  2C0D0DAD28867E10 CRC64;
     MLSVPHLDRD FDYLVPAEHS DDAQPGVRVR VRFHGRLVDG FVLERRSDSD HHGKLGWLDR
     VVSPEPVLTT EIRRLVDAVA ARYAGTRQDV LRLAVPARHA RVEREITTAP GRPVVAPVDP
     SGWAAYGRGR QFLAALADSR AARAVWQALP GELWADRFAE AAAQTVRAGR TVLAIVPDQR
     DLDTLWQAAT ALVDEHSVVA LSAGLGPEAR YRRWLAALRG SARLVIGTRS AVFAPLSELG
     LVMVWADADD SLAEPRAPYP HAREVAMLRA HQARCAALIG GYARTAEAHA LVRSGWAHDV
     VAPRPEVRAR SPRVVALDDS GYDDARDPAA RTARLPSIAL RAARSALQSG APVLVQVPRR
     GYIPSLACGR CRAIARCRSC TGPLSLQGAG SPGAVCRWCG RVDPTLRCVR CGSDVVRAVV
     VGARRTAEEL GRAFPGTAVI TSAGDTLVPQ LDAGPALVVA TPGAEPRAPG GYGAALLLDS
     WALLGRQDLR AAEDALWRWM TAAALVRPRG AGGVVTVVAE SSIPTVQSLI RWDPVGHAEA
     ELAARTEVGL PPSVHIAALD GPAGTVTALL EAARLPDPDR LQADLLGPVD LPPGVRRPAG
     IPADAPVIRM LLRVCREQGL ELAASLRRGI GVLSARQTRQ TRSLVRVQID PLHIG