ID   PRIA_MYCLE              Reviewed;         651 AA.
AC   Q9CCQ3;
DT   14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Probable primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983}; OrderedLocusNames=ML0548;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- CAUTION: Compared to other bacterial PriA, it has a very divergent
CC       helicase domain. {ECO:0000305}.
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DR   EMBL; AL583918; CAC30056.1; -; Genomic_DNA.
DR   PIR; D86977; D86977.
DR   RefSeq; NP_301464.1; NC_002677.1.
DR   RefSeq; WP_010907788.1; NC_002677.1.
DR   AlphaFoldDB; Q9CCQ3; -.
DR   SMR; Q9CCQ3; -.
DR   STRING; 272631.ML0548; -.
DR   EnsemblBacteria; CAC30056; CAC30056; CAC30056.
DR   KEGG; mle:ML0548; -.
DR   PATRIC; fig|272631.5.peg.955; -.
DR   Leproma; ML0548; -.
DR   eggNOG; COG1198; Bacteria.
DR   HOGENOM; CLU_015485_1_0_11; -.
DR   OMA; CTVEAAQ; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1440.60; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   Pfam; PF17764; PriA_3primeBD; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Primosome; Reference proteome; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..651
FT                   /note="Probable primosomal protein N'"
FT                   /id="PRO_0000102128"
FT   ZN_FING         371..383
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         399..414
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
SQ   SEQUENCE   651 AA;  69923 MW;  06CFE91D208C7043 CRC64;
     MLSVPHLDRE FDYLVSAEQS DDAQPGVRVR VRFHGRLVDG FVLERRNDTE HFGKLGWLDR
     VVSAQPVLTA EVRRLVDAVV ARYAGTRPDV LRLAVPTRHA RVERETLAIP VSPLPLIPGP
     VDPSGWEVYG RGGQFLTALA ESRAARAVWQ ALPGEQWADR FAEAAAQAIR AGRAALAIVP
     DQRDLDVLWR AVTTRVDERS VVALSAGLGQ AVRYQRWLKV LRGSARLVIG TRSAVFAPVN
     DLGLVMVWSD ADDMLAEPRA PYPHAREVAM LRAYQARCAA LIGGYTRTAE AHALVRSGWA
     HDVVAARSVV RARAPRVVAL DDSGYAEESD PAARTARLPS IALRAARSAL AAGAPVLVQV
     PRRGYVPSLA CGRCRTLARC RHCTGPLSLL DRATPGTVCC WCGRADLTLR CARCGSEVVR
     AVVVGARRTA EELGRAFAGM PVITSVGDTI VPEVGARPAL VVATPGAEPR APGGYGAALL
     LDTWALLGRQ DLRAAEEALW RWMTAAALVR ARGDGGVVMV VAEASIPTVQ SLMRWDPASH
     AEAELAARTE VGLPPSVHIA AVDGTTGAVN ELLQEARLPD EADLLGPVDL PQGVRRPAGT
     PLGAPISRLL VRVPREQGWQ LAASLRRGIG VLSVRQTHQL VRVQIDPLHI G