ID   PRIA_MYCTO              Reviewed;         654 AA.
AC   P9WMQ8; L0T6J5; P0A5A5; P71670;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Probable primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983}; OrderedLocusNames=MT1446;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- CAUTION: Compared to other bacterial PriA, it has a very divergent
CC       helicase domain. {ECO:0000305}.
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DR   EMBL; AE000516; AAK45711.1; -; Genomic_DNA.
DR   PIR; G70900; G70900.
DR   RefSeq; WP_010924396.1; NC_002755.2.
DR   AlphaFoldDB; P9WMQ8; -.
DR   SMR; P9WMQ8; -.
DR   EnsemblBacteria; AAK45711; AAK45711; MT1446.
DR   KEGG; mtc:MT1446; -.
DR   HOGENOM; CLU_015485_1_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1440.60; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   Pfam; PF17764; PriA_3primeBD; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Primosome; Zinc; Zinc-finger.
FT   CHAIN           1..654
FT                   /note="Probable primosomal protein N'"
FT                   /id="PRO_0000427261"
FT   ZN_FING         367..379
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         395..410
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
SQ   SEQUENCE   654 AA;  69962 MW;  E3CE69668350DED0 CRC64;
     MLSVPHLDRD FDYLVPAEHS DDAQPGVRVR VRFHGRLVDG FVLERRSDSD HHGKLGWLDR
     VVSPEPVLTT EIRRLVDAVA ARYAGTRQDV LRLAVPARHA RVEREITTAP GRPVVAPVDP
     SGWAAYGRGR QFLAALADSR AARAVWQALP GELWADRFAE AAAQTVRAGR TVLAIVPDQR
     DLDTLWQAAT ALVDEHSVVA LSAGLGPEAR YRRWLAALRG SARLVIGTRS AVFAPLSELG
     LVMVWADADD SLAEPRAPYP HAREVAMLRA HQARCAALIG GYARTAEAHA LVRSGWAHDV
     VAPRPEVRAR SPRVVALDDS GYDDARDPAA RTARLPSYRW RRGSALQSGA PVLVQVPRRG
     YIPSLACGRC RAIARCRSCT GPLSLQGAGS PGAVCRWCGR VDPTLRCVRC GSDVVRAVVV
     GARRTAEELG RAFPGTAVIT SAGDTLVPQL DAGPALVVAT PGAEPRAPGG YGAALLLDSW
     ALLGRQDLRA AEDALWRWMT AAALVRPRGA GGVVTVVAES SIPTVQSLIR WDPVGHAEAE
     LAARTEVGLP PSVHIAALDG PAGTVTALLE AARLPDPDRL QADLLGPVDL PPGVRRPAGI
     PADAPVIRML LRVCREQGLE LAASLRRGIG VLSARQTRQT RSLVRVQIDP LHIG