ID   PRIA_RICFE              Reviewed;         648 AA.
AC   Q4UL64;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983}; OrderedLocusNames=RF_0858;
OS   Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=315456;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-1525 / URRWXCal2;
RX   PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA   Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA   Parinello H., Claverie J.-M., Raoult D.;
RT   "The genome sequence of Rickettsia felis identifies the first putative
RT   conjugative plasmid in an obligate intracellular parasite.";
RL   PLoS Biol. 3:1-12(2005).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
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DR   EMBL; CP000053; AAY61709.1; -; Genomic_DNA.
DR   RefSeq; WP_011271186.1; NC_007109.1.
DR   AlphaFoldDB; Q4UL64; -.
DR   SMR; Q4UL64; -.
DR   STRING; 315456.RF_0858; -.
DR   EnsemblBacteria; AAY61709; AAY61709; RF_0858.
DR   KEGG; rfe:RF_0858; -.
DR   eggNOG; COG1198; Bacteria.
DR   HOGENOM; CLU_013353_4_0_5; -.
DR   OMA; RCHYCGY; -.
DR   OrthoDB; 1132322at2; -.
DR   Proteomes; UP000008548; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1440.60; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   InterPro; IPR041236; PriA_C.
DR   InterPro; IPR040498; PriA_CRR.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF17764; PriA_3primeBD; 1.
DR   Pfam; PF18074; PriA_C; 1.
DR   Pfam; PF18319; PriA_CRR; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00595; priA; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Primosome; Zinc; Zinc-finger.
FT   CHAIN           1..648
FT                   /note="Primosomal protein N'"
FT                   /id="PRO_0000281070"
FT   DOMAIN          131..297
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   DOMAIN          393..548
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         358..370
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   ZN_FING         385..401
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT   MOTIF           240..243
FT                   /note="DEAH box"
FT   BINDING         144..151
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
SQ   SEQUENCE   648 AA;  72948 MW;  E578F6B518940703 CRC64;
     MRIAKILLPA AKLFPLDYLI PESLVLNIGD LVIVPFRSKE LTGIVWEFAT IPEASKLKTV
     KEKVPLDLSI TSEVLELIKW LSSYYMSELG SIAKLVLPID IAEKPIKVKE QKVNNSFVLP
     DLSEEQKQAV TILNESNKPT LIKGVTGSGK TEIYFHLIAD YLVKGKQVLI MLPEIALSTQ
     IINRFIERFG FEPIIWNSSV TKAQKKMILR GILSDKVKVV IGARSSLFLP FKNLGLVVID
     EEHDDSYKQD DGILYNARDT AIVRGTFDKA QIVLCSATPS IETMYNIEIG KYQLVTLVNR
     YNNVDLPNIE IIDMTKEKLS KNSYLSKILI EAIKDNLDNK KQVLLFLNRR GYAPLMLCKA
     CGHRFTCKFC SSWMVVHKAT KKLECHHCGY QSKIFSSCPE CLEDETLTIC GPGIERIEEE
     AKALFPENKI AVISKDHAKN PERIAQLLHQ MENLEIDILI GTQMITKGYH FPNLTLVGVI
     DADLGSNNAD LRASERTFQL LHQVGGRAGR GDGKGVVYLQ SYYPDNIIFS YVKAGDEDSF
     FANELEIRKS ADMPPFSKTA SVILSGSNES KILEIARAMV RIAPKANVKI LGPASSLMSK
     LAGKYRYRIL IIADKKFNLQ KYLKFWLSLI KIPSFCHLKI DIDPKSFY