PRIA_SYNY3
ID PRIA_SYNY3 Reviewed; 831 AA.
AC P74397;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN Name=priA {ECO:0000255|HAMAP-Rule:MF_00983}; OrderedLocusNames=sll0270;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- FUNCTION: Involved in the restart of stalled replication forks.
CC Recognizes and binds the arrested nascent DNA chain at stalled
CC replication forks. It can open the DNA duplex, via its helicase
CC activity, and promote assembly of the primosome and loading of the
CC major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC Rule:MF_00983}.
CC -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-Rule:MF_00983}.
CC -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00983}.
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DR EMBL; BA000022; BAA18494.1; -; Genomic_DNA.
DR PIR; S76235; S76235.
DR AlphaFoldDB; P74397; -.
DR SMR; P74397; -.
DR IntAct; P74397; 5.
DR STRING; 1148.1653581; -.
DR PaxDb; P74397; -.
DR EnsemblBacteria; BAA18494; BAA18494; BAA18494.
DR KEGG; syn:sll0270; -.
DR eggNOG; COG1198; Bacteria.
DR InParanoid; P74397; -.
DR OMA; RCHYCGY; -.
DR PhylomeDB; P74397; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR Gene3D; 3.40.1440.60; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00983; PriA; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005259; PriA.
DR InterPro; IPR041222; PriA_3primeBD.
DR InterPro; IPR042115; PriA_3primeBD_sf.
DR InterPro; IPR041236; PriA_C.
DR InterPro; IPR040498; PriA_CRR.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF17764; PriA_3primeBD; 1.
DR Pfam; PF18074; PriA_C; 1.
DR Pfam; PF18319; PriA_CRR; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00595; priA; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA replication; DNA-binding; Helicase; Hydrolase;
KW Metal-binding; Nucleotide-binding; Primosome; Reference proteome; Zinc;
KW Zinc-finger.
FT CHAIN 1..831
FT /note="Primosomal protein N'"
FT /id="PRO_0000102134"
FT DOMAIN 304..471
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT DOMAIN 575..735
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT ZN_FING 537..549
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT ZN_FING 568..584
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
FT MOTIF 413..416
FT /note="DEAH box"
FT BINDING 317..324
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00983"
SQ SEQUENCE 831 AA; 93307 MW; EA52CDF95654D94A CRC64;
MTVSPSALAE LGFNYQEEDS VRPWAAVLVD LPQNEGVYTY AIPPGLTVQD GDIVAVPFGN
QQLGGIVVGC LTKLPPDLPP EKIKPIQDVI VSGFFAPHYW RLLHWLAEYY CTELITVIRM
ALPPGLLQRS QRRIKLNGDR LPSDWPLFLS QPSHQGARQI LTLLQSSKEG DYSYRYLRQK
VPGLTKALRD LLKRQWVESY LEPPKAVQPQ LPKMVTLLNF DPSFKLTELQ ARTLIVLKNQ
GGEMWLADLL KAVPCSASTI QSLAKKGLVA IAEREKLRLF QQPSINASQA PELTPAQKQA
CQTVLPLQGY HQVLLHGVTG SGKTEVYLQI CGDRLGKGQS VLVLVPEIGL TPQLTDRFRA
RFGNKVAVYH SGLSSGEKYD TWRQTLLGHE QIVIGTRSAI FTPLPNLGLI ILDEEHDSSF
KQTQLTPNYH ARTVAQRRAE LEQCPLILGS ATPSLESWHT VHRHQNDPQR HYLELPERVQ
SRPLPPVQIV DMRAELKQGN RSIFSRALQT ALGELKAKQQ QGILFINRRG HSTFVSCRSC
GYVLECPNCD VSLSYHYVQG NGQPLLRCHY CNHAEIQPKV CPECSSPYLK FFGSGTQKVT
EALTQEFPDL RWIRFDSDTT RRKGAHRALL DQFQRGEADL LVGTQMLTKG LDLAQITLVG
VVAADSLLNF ADYRSAERGF QTLTQVSGRA GRGEEPGQVI IQTYTPRHPV IQAVKTHNYH
GFIAQELPQR EMLNYPPYGK LILLRCLGTN EREVEKTIQA IAVLCEQLLP KTVEILGPAP
ASILRIAQRY RWQLLVKYPS FIKVILPLER IKQICPSSVY LDIDVDPLSI D
