PRIH_ARATH
ID PRIH_ARATH Reviewed; 337 AA.
AC F4I6E6; Q9SA81;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Primase homolog protein;
DE EC=2.7.7.-;
GN OrderedLocusNames=At1g30660; ORFNames=T5I8.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION.
RX PubMed=23452619; DOI=10.1186/1471-2229-13-36;
RA Diray-Arce J., Liu B., Cupp J.D., Hunt T., Nielsen B.L.;
RT "The Arabidopsis At1g30680 gene encodes a homologue to the phage T7 gp4
RT protein that has both DNA primase and DNA helicase activities.";
RL BMC Plant Biol. 13:36-36(2013).
CC -!- FUNCTION: May act as a DNA primase. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds two Mg(2+) per subunit. {ECO:0000250};
CC -!- CAUTION: This truncated primase was identified on the basis of sequence
CC homologies, but no information is available about whether this protein
CC is functional. {ECO:0000305|PubMed:23452619}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD25753.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007060; AAD25753.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31257.1; -; Genomic_DNA.
DR PIR; A86432; A86432.
DR RefSeq; NP_174354.4; NM_102803.4.
DR AlphaFoldDB; F4I6E6; -.
DR SMR; F4I6E6; -.
DR STRING; 3702.AT1G30660.1; -.
DR PaxDb; F4I6E6; -.
DR PRIDE; F4I6E6; -.
DR ProteomicsDB; 236601; -.
DR EnsemblPlants; AT1G30660.1; AT1G30660.1; AT1G30660.
DR GeneID; 839946; -.
DR Gramene; AT1G30660.1; AT1G30660.1; AT1G30660.
DR KEGG; ath:AT1G30660; -.
DR Araport; AT1G30660; -.
DR TAIR; locus:2204559; AT1G30660.
DR eggNOG; ENOG502QPXS; Eukaryota.
DR HOGENOM; CLU_070942_0_0_1; -.
DR InParanoid; F4I6E6; -.
DR OrthoDB; 212176at2759; -.
DR PRO; PR:F4I6E6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I6E6; baseline and differential.
DR Genevisible; F4I6E6; AT.
DR GO; GO:0043139; F:5'-3' DNA helicase activity; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR CDD; cd01029; TOPRIM_primases; 1.
DR InterPro; IPR034154; TOPRIM_DnaG/twinkle.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR027032; Twinkle-like.
DR PANTHER; PTHR12873; PTHR12873; 1.
DR Pfam; PF13662; Toprim_4; 1.
DR SMART; SM00493; TOPRIM; 1.
PE 3: Inferred from homology;
KW Magnesium; Metal-binding; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..337
FT /note="Primase homolog protein"
FT /id="PRO_0000422120"
FT DOMAIN 205..304
FT /note="Toprim"
FT BINDING 211
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 273
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 273
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 275
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 337 AA; 37807 MW; 758F0861D0E0D183 CRC64;
MSENVPDHQS TNGFSSSDFF VPEEEAGKKV VLSKLVTLMR KLSEQGIDAQ NCPPGVRSCL
ICPKCEVGDS GEKSLTLYIY PDGSSAKWTC RRKCGLKGVL QVDGKLVSKD PIGKVERKIT
VESIKLEPLC DEIQDFFAAR AISGKTLERN RVMQKRIDDE IVIAFTYWQR GELVSCKYRS
LTKKFVQERN TRKILYGLDD IEETSEIIIV EGEPDKLAME EAGFFNCVSV PDGAPETVSS
KEIPSESKDT AFKYIWNCND YLKKASRIVI ATDGDGPGQA LAEELARRLG KERCWLVKWP
KKSEDEHFKD ANEVLMSKGP HLLKEAILNA EPYPLKS
