ID   PRIM_ALHV1              Reviewed;         837 AA.
AC   O36406;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   02-DEC-2020, entry version 60.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=56;
OS   Alcelaphine herpesvirus 1 (strain C500) (AlHV-1) (Malignant catarrhal fever
OS   virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Macavirus.
OX   NCBI_TaxID=654901;
OH   NCBI_TaxID=9927; Connochaetes taurinus (Blue wildebeest).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9261371; DOI=10.1128/jvi.71.9.6517-6525.1997;
RA   Ensser A., Pflanz R., Fleckenstein B.;
RT   "Primary structure of the alcelaphine herpesvirus 1 genome.";
RL   J. Virol. 71:6517-6525(1997).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; AF005370; AAC58103.1; -; Genomic_DNA.
DR   PIR; T03151; T03151.
DR   RefSeq; NP_065555.1; NC_002531.1.
DR   GeneID; 911780; -.
DR   KEGG; vg:911780; -.
DR   Proteomes; UP000000941; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..837
FT                   /note="DNA primase"
FT                   /id="PRO_0000405742"
FT   ZN_FING         782..821
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            498
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            500
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   837 AA;  95341 MW;  620255A61104016A CRC64;
     MTRVVKALFA ADTDCAELLA DILTGQNNSG SLYCLLHNCR PEDVSWEVTR LSLCLPVRRP
     GGAEGRCAEV FKIKLPTEEA GQFLFNCGSL SAKQVYESYS KRAAYETFQP ILEVLGLSGD
     EYSIKNAVTW GRGKFVASLR KYFKLTTSPH WFINTFGAFE NHFVLVSSCY YFFPVSVSTV
     DTLCHLAMLY SSKKGCPLSS ITTLRELGAV ATQSPALDRV ENFYLYVCDK LARDTLECEA
     VDRCINEFRG QLMLSDQDLV HYIYLSFFQC FNNQKFLAYS QCTNPCNLNT TMLREPMLVA
     NIDSDFKHKM ATYYNKNTYL SNYVLLRGIH LHPVVGYGQE CLRSAHATGG HSIWWGESHQ
     VSDMLKTINV EYPDICLHEE FRGLMDLAAI TDRCSIFGNP IHYLTDCATS GAIPIYRSEL
     SHRHYFLAVF SDDIEYFWKK TIFLPPESFC LGAQDTMLTR AITYTEMHCS MSSVAEQIHV
     SRHEYFNPKL PVFNWVLDLD LPISEGNLHI DSIYSLCLLI RESVLDILKL LGPVEPDHEV
     FFFKSACINL CDPGEASWRP STFCTCTEKL GMRVVTRFPP GICLKGSEPL TQLTKILNRV
     IKLGCGSLLN LSAFQLSNGP FDVGIYGRGR SIRLPHTYKV GKCGQLERLL KLFVCHPEAT
     DKFPYLQNSL KLNRLLHHAQ SQDPRGPLKI IYRVEDINED FLYKHTQKQL PVKHEAVIPS
     IERLLDTSLN CFLLSKVWPK CFGTIRSYMS EEKLQQFSRV VFHPTNHCIV QVRPDRGNNF
     KCLRYNHRGS SKSVRVFLIL HLKEETKLIV TFMSQCFANK CQSNKAMAHF SVFVDLS