ID   PRIM_EBVB9              Reviewed;         874 AA.
AC   P03193; Q777F5;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   ORFNames=BSLF1;
OS   Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus.
OX   NCBI_TaxID=10377;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6087149; DOI=10.1038/310207a0;
RA   Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J.,
RA   Gibson T.J., Hatfull G., Hudson G.S., Satchwell S.C., Seguin C.,
RA   Tuffnell P.S., Barrell B.G.;
RT   "DNA sequence and expression of the B95-8 Epstein-Barr virus genome.";
RL   Nature 310:207-211(1984).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; V01555; CAA24848.1; -; Genomic_DNA.
DR   EMBL; AJ507799; CAD53412.1; -; Genomic_DNA.
DR   PIR; D43041; QQBE15.
DR   RefSeq; YP_401662.1; NC_007605.1.
DR   BioGRID; 971772; 1.
DR   IntAct; P03193; 3.
DR   MINT; P03193; -.
DR   PRIDE; P03193; -.
DR   DNASU; 3783730; -.
DR   GeneID; 3783730; -.
DR   KEGG; vg:3783730; -.
DR   Proteomes; UP000153037; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IDA:UniProtKB.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..874
FT                   /note="DNA primase"
FT                   /id="PRO_0000116113"
FT   ZN_FING         786..824
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   REGION          848..874
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            503
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            505
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   874 AA;  98041 MW;  4B89429FC5E4DD03 CRC64;
     MSAPVVIKAL VASNTDIAEA ILDAILSRPD EGFRLFCLCH NASPLHHVAG SLVELQLHLP
     KKRLTSQSRC GLVLTLHLPA EEAFPFLRGL TPLTADRLST YLDRAGALRS LTPLVELLTL
     SAKKQPQGDA RGRVAWLRPK IVGCLRRIYR VNISARWFIS TFGSHEAQFV LVTAAYYFWG
     IPCTIETLAH LTELFTSESG QSLAAVTSLA ELGEVFGSSA WAEQTEAFAH FAHEKLRRDS
     REIRAVARTI DAYRGRLPLA SADLVRYVYL AHAQCFNEGT FKRYSQLTSM GEIGCLPSGG
     VVLPSLLDRG FAEHMRTYFT RETYLAEHVR VQQLKIRMEP PAPYTWDPDP DDGLMRAWAG
     LSVDVARELV ELARWHADEG PTYPPTLQGF LCLAGQATCR GQWNPKEQFL PPTVLRRVQR
     LPVFLCHFAD RHYFVMTAAD PFSSHLAEVV STPTNCRLPD TCLTRALSYT PVYYSQNSLS
     EQLFVSRHEY FNPRLPVCNL VLDLDLKIKG APWSLEEIYD LCRTVRREVL RLMRRLGPVS
     RAHPVYFFKS ACPPADPDNM EDVLPFCICT GKLGFRVITP LPRGHAIVGT SAVQGFVSVL
     QKLMGLTACL RRMRHKIKEI GAPLFDSGVY HAGRCIRLPH TYKVDRGGGL SRQLRLFVCH
     PEEEDKHSYV KNALNIQNLL HHSLHVGWPA PKTFCYHIAD DGRDYLIQRT RETLPPTVEN
     VCAMIEGHLG LDLVAWVSSC IWPSLMSTLA TAVPEDKFPQ FLHVTFEQTG PNLVQVCHAR
     GRNFACLRHT HRASSKNVRV FLVLYYTSQA ITVTFMSQCF AGRCGANQPT AHFSISVPAS
     RIINRAEASQ DSTTSQLARR RDRQDGSFSE TLPN