PRIM_EHV1B
ID PRIM_EHV1B Reviewed; 1081 AA.
AC P28962; Q6S6R4;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 23-FEB-2022, entry version 78.
DE RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN OrderedLocusNames=7;
OS Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX NCBI_TaxID=31520;
OH NCBI_TaxID=9796; Equus caballus (Horse).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=1318606; DOI=10.1016/0042-6822(92)90706-u;
RA Telford E.A.R., Watson M.S., McBride K., Davison A.J.;
RT "The DNA sequence of equine herpesvirus-1.";
RL Virology 189:304-316(1992).
CC -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC the DNA at the replication forks and generates single-stranded DNA for
CC both leading and lagging strand synthesis. The primase initiates primer
CC synthesis and thereby produces large amount of short RNA primers on the
CC lagging strand that the polymerase elongates using dNTPs.
CC {ECO:0000255|HAMAP-Rule:MF_04011}.
CC -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC Note=Requires the presence of the primase associated factor to properly
CC localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR EMBL; AY665713; AAT67264.1; -; Genomic_DNA.
DR PIR; H36795; WZBEA6.
DR RefSeq; YP_053052.1; NC_001491.2.
DR PRIDE; P28962; -.
DR GeneID; 1487515; -.
DR KEGG; vg:1487515; -.
DR Proteomes; UP000001189; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04011; HSV_PRIM; 1.
DR InterPro; IPR033685; HSV_PRIM.
PE 3: Inferred from homology;
KW DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW Transferase; Zinc; Zinc-finger.
FT CHAIN 1..1081
FT /note="DNA primase"
FT /id="PRO_0000116105"
FT ZN_FING 1024..1064
FT /note="CHC2-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT REGION 484..508
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 652
FT /note="Essential for primase activity"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT SITE 654
FT /note="Essential for primase activity"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ SEQUENCE 1081 AA; 118963 MW; C76D42F36ADC7BC3 CRC64;
MAQRNPEPTI RVLYATDSCV ITYSLMLLTG QESSEGVYAI SYDWSSELDD LFGRQPRAPN
TDAGDGWSST EQSDADQLAT ALLQRKPSVS FCLLSGMVGG ASDEPQDRVR PMFVCVFSTW
TGARALAMTL SHGHPLSSNT LLQALTEEAT FLLHNDLILA LAITTENVTA RSGRTAAAAK
YDPQRGSVKA AVIGHSTGRS GLTSVYIHHE NKVLTAFRRL YCNNNTTPFW FASKFGPGER
EIVLATRYYL FQAIRCSRSG GTYDLQAVKD FIRTYNVPAA PNPTGLDLTH LTSFSLLSKF
CCQSWYSRGP CALALPRYVD LRIQADVAEV SALEGFIAAD RQGLRVSDRE FITYIYLAHF
ESFNRKQLYD HLLAVSIADP GDIDRITSTS SLKRGTIEKF FAQVRIQLNI RDYIAHNVNP
RVVCLPASIG SQYAQDKTYT PSSTTMSTGS APLGVCDTST PILKLLDRVE SSLAGRGWIQ
TIVSPNKPQS VHSTPPLDQS RGDELSPGVS SQCGISRRLL HIASSPPVNG RALPLEVLFG
QKGVPGPAPV YRVALPSKRQ AFAVIANDRW ETTTQNLARP GGSKQAYEGG FALAGFGEID
DCSLAWRDLQ LTRTTSGVCR TALASSNASA QMYINRNEIF NSSLAVSNII LDVDFGIKRR
VPLGMLHLAM RGFRAGIITT LSLIFSDATV QWDSYPCYFY KTSCPPQLVR ALHRGEPSSF
PDYVDGVEEC YMESDFIDDY AAMEEYTDGP MDDYEMMMVD NECPQAACDN TPPNKEGGKT
PLQRLDDTDA CECTEKMGFR VTVPVPPPYL LAGPEALRGL ARIIQQAVVL ERTFTESMCS
VLRDFSFIDT GVYSHGRSLR LPFFCKVGDS GEVYGGLYPF YVIPPKCNDV DEFVAQHSNP
ANFHFHAAPR HPTITHVITD LGGDYVSFFE RKVARNREAI MTKRATLESL LSSANVSIKS
HEAVEAFVVD VVLGEVVSHL ATHFPDQSGE YQTVGVHTVV TKPDWILMQI NRSGNAYRSQ
GFSCLRAKHL RSARGLARTF LSISADVHGR LCASISQQCF ATKCGNNKMC TIFTLEVDRA
K