ID   PRIM_EHV1V              Reviewed;        1081 AA.
AC   P84401; Q6S6R4;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   23-FEB-2022, entry version 44.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   OrderedLocusNames=7;
OS   Equine herpesvirus 1 (strain V592) (EHV-1) (Equine abortion virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=310273;
OH   NCBI_TaxID=9796; Equus caballus (Horse).
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAS45891.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Davis-Poynter N., Nugent J., Birch-Machin I., Allen G.P.;
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; AY464052; AAS45891.1; -; Genomic_DNA.
DR   RefSeq; YP_053052.1; NC_001491.2.
DR   GeneID; 1487515; -.
DR   KEGG; vg:1487515; -.
DR   Proteomes; UP000008296; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Transferase; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..1081
FT                   /note="DNA primase"
FT                   /id="PRO_0000116106"
FT   ZN_FING         1024..1064
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   REGION          484..508
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            652
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            654
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   1081 AA;  118963 MW;  C76D42F36ADC7BC3 CRC64;
     MAQRNPEPTI RVLYATDSCV ITYSLMLLTG QESSEGVYAI SYDWSSELDD LFGRQPRAPN
     TDAGDGWSST EQSDADQLAT ALLQRKPSVS FCLLSGMVGG ASDEPQDRVR PMFVCVFSTW
     TGARALAMTL SHGHPLSSNT LLQALTEEAT FLLHNDLILA LAITTENVTA RSGRTAAAAK
     YDPQRGSVKA AVIGHSTGRS GLTSVYIHHE NKVLTAFRRL YCNNNTTPFW FASKFGPGER
     EIVLATRYYL FQAIRCSRSG GTYDLQAVKD FIRTYNVPAA PNPTGLDLTH LTSFSLLSKF
     CCQSWYSRGP CALALPRYVD LRIQADVAEV SALEGFIAAD RQGLRVSDRE FITYIYLAHF
     ESFNRKQLYD HLLAVSIADP GDIDRITSTS SLKRGTIEKF FAQVRIQLNI RDYIAHNVNP
     RVVCLPASIG SQYAQDKTYT PSSTTMSTGS APLGVCDTST PILKLLDRVE SSLAGRGWIQ
     TIVSPNKPQS VHSTPPLDQS RGDELSPGVS SQCGISRRLL HIASSPPVNG RALPLEVLFG
     QKGVPGPAPV YRVALPSKRQ AFAVIANDRW ETTTQNLARP GGSKQAYEGG FALAGFGEID
     DCSLAWRDLQ LTRTTSGVCR TALASSNASA QMYINRNEIF NSSLAVSNII LDVDFGIKRR
     VPLGMLHLAM RGFRAGIITT LSLIFSDATV QWDSYPCYFY KTSCPPQLVR ALHRGEPSSF
     PDYVDGVEEC YMESDFIDDY AAMEEYTDGP MDDYEMMMVD NECPQAACDN TPPNKEGGKT
     PLQRLDDTDA CECTEKMGFR VTVPVPPPYL LAGPEALRGL ARIIQQAVVL ERTFTESMCS
     VLRDFSFIDT GVYSHGRSLR LPFFCKVGDS GEVYGGLYPF YVIPPKCNDV DEFVAQHSNP
     ANFHFHAAPR HPTITHVITD LGGDYVSFFE RKVARNREAI MTKRATLESL LSSANVSIKS
     HEAVEAFVVD VVLGEVVSHL ATHFPDQSGE YQTVGVHTVV TKPDWILMQI NRSGNAYRSQ
     GFSCLRAKHL RSARGLARTF LSISADVHGR LCASISQQCF ATKCGNNKMC TIFTLEVDRA
     K