ID   PRIM_EHV2               Reviewed;         884 AA.
AC   Q66658;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 68.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=56;
OS   Equine herpesvirus 2 (strain 86/87) (EHV-2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Percavirus.
OX   NCBI_TaxID=82831;
OH   NCBI_TaxID=9796; Equus caballus (Horse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=7783207; DOI=10.1006/jmbi.1995.0314;
RA   Telford E.A.R., Watson M.S., Aird H.C., Perry J., Davison A.J.;
RT   "The DNA sequence of equine herpesvirus 2.";
RL   J. Mol. Biol. 249:520-528(1995).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; U20824; AAC13844.1; -; Genomic_DNA.
DR   PIR; S55651; S55651.
DR   RefSeq; NP_042653.1; NC_001650.2.
DR   PRIDE; Q66658; -.
DR   GeneID; 1461069; -.
DR   KEGG; vg:1461069; -.
DR   Proteomes; UP000007083; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; Helicase; Host nucleus; Hydrolase;
KW   Metal-binding; Nucleotide-binding; Reference proteome; Transferase; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..884
FT                   /note="DNA primase"
FT                   /id="PRO_0000406066"
FT   ZN_FING         825..864
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            529
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            531
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   884 AA;  98684 MW;  76833888B1825537 CRC64;
     MATGRSTSTE SSEKTCQQRD PLPEYRALCG TDGDAAEILT DVLTNTDSDG VVFCLAHNCY
     SYNIGGGEAL LTLCLPAKRP WGAEKCLPVI QFRCDASRAQ EFLFQGRPIP VRYIQTNLNH
     RAVKKFFKPI LSVLTCSDKK GGGGEGAHAD LKSTIFWFRA KFVAAVRKTF KITASPFWMI
     STFGCTEAQF VLVSSCYFFE RHECTIDTLS HLSRLFDGSR GRQLTTVNTF SDLAGMFGTS
     AWLGRVPEFS AYVGKKLARD DLESAAVDEA VNAFRGQLML SNADLIHYIY LSFFQCLNKE
     KFLEYSLRTN PHNIDGVPPE EPIITGFIDE GFKSKMATYY TKSSYLKNHV RVGSLYLDGV
     EGYSPEAIEA GPPAAAGGGG GADRYWAGQS RDVQGLLSDI LADHPASRLS PDLHGLLDLA
     ALGDSSGVAG GVKDSLFPEP LRCPVYRCQY LNKTFFAVVT RDNLARAWER AVQLPTQVTG
     WEGMEDARLT ACVHYAELAF SLGHLREQLS VSRHEYFNPR LPVFNLVLDF DLPLKKPGLS
     LERVYSICRS VRSDVLSVLG VLGEVDEAAH PVYFFKSACP RPEWDEPYAG RPFCTCDAKL
     GLRIITPLPR GVAIVGGAPL VALAKILNRM IKMNREDLLE ICPGLPDADG PLDTGIYHRG
     RCVRLPHTYK VNEACGLERL LRLFVCHPGS PDKAAYIRDA MTLRNLLHHS KSAYWEGNAR
     EGVSEEAPQK TKVVYSVTDV SENFLVCQTQ QQLPRSYERP DSRIETMTGR DLVTWVTEVA
     WPKVFHNIKA YIPDDKTTQF HFVKFIHTSH NIIQVKPQRG NNFVCISSNH RNKTQSVRIF
     IVLYTNKKDE VTITLMSQCF AHKCNSNKPR AHFSIPLQLR GRDF