ID   PRIM_GAHVM              Reviewed;        1074 AA.
AC   Q9E6M4;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   23-FEB-2022, entry version 62.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=MDV066;
OS   Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's
OS   disease herpesvirus type 1).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Mardivirus.
OX   NCBI_TaxID=10389;
OH   NCBI_TaxID=9031; Gallus gallus (Chicken).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10933706; DOI=10.1128/jvi.74.17.7980-7988.2000;
RA   Tulman E.R., Afonso C.L., Lu Z., Zsak L., Rock D.L., Kutish G.F.;
RT   "The genome of a very virulent Marek's disease virus.";
RL   J. Virol. 74:7980-7988(2000).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; AF243438; AAG14246.1; -; Genomic_DNA.
DR   RefSeq; YP_001033982.1; NC_002229.3.
DR   PRIDE; Q9E6M4; -.
DR   GeneID; 4811527; -.
DR   KEGG; vg:4811527; -.
DR   Proteomes; UP000008072; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..1074
FT                   /note="DNA primase"
FT                   /id="PRO_0000406558"
FT   ZN_FING         1012..1052
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            659
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            661
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   1074 AA;  120176 MW;  82C44B0125A161CE CRC64;
     MARFSSISDT LESDDSGIKV LFAVDGCAVS FSLALLTGQI PSTNSVYVIG YWDPSDRFSS
     IPFLDGDPNT NERISTTVCN LEDVPSPLRV EFCLLNQMAS GMGGADLKLR TRAIFVCRFT
     SWSEMNAIAN SIIYGTPIQA GVLQATISET ETFMLHDEFN LALHVFLNGL SLKGRNKKDV
     CMSLNHNYIS SVSENFPRGK RGLTGLYLQH EQKVTAAYRR IYGGSTTTAF WYVSKFGPDE
     KSLVLALRYY LLQAQEEVTG IATGYDLQAI KDICKTYAVS VNPNPTGFLA ADLTSFSRLS
     RFCCLSYYSK GSVAIAFPSY VERRIMADIA EVDALREYIE RDRPSLKISD LEFVKYIYLA
     YFECYNREQL KRHLKDVTVS LPDEDIYKKS SLGKCAVENF FTHVRSRLNV NDHIAHNVLP
     EQVEMGNKLV RKFGRARMYL STTMTNESHF TGICECASVI LKRLDTLEMK LQKYGWPSDR
     VDGSNLMADN QNNSTLIPYD KSRSSGMILE CSNTHSRGGP MIVKRLLALV SADSRAGGIG
     PANMLMGIDS AIDGPLPVYR VGMSKGRQAF TVLMTECWER TIPSPGSAKA HLIKLNNSYG
     TSTEDLISRD LFLTSEIEQL IGSTVELPEI TCGSADEQQY INRNEVFNGN LAIGNIVLDV
     DIHLRNPIPL RLMHAAIRGF RSGILRALAL LLPKANIDHG SYPCYFYKSS CKKSRVMGGA
     PWMLHDAELA PDYSMFENAE FDLEMGIDDP LLIDQIDESL TRWSSESSRS VDLDPDKPCG
     CHDKIGLRVC IPVPSPYLLV GSKTLAGLSR IIQQAVLLER NFVETIGPYL KNYEIIDSGV
     YGHGRSLRLP FFGKIDENGI VSRRLVPFFV IPDDCADMEK FIVAHFEPKN FHFHSSIPLE
     KAAIILKDIG GEYAGFFERK ITVNRDIFFG TRLSLSIALR ERGVDINDCA AITTFVTDHI
     LDDIITYVYE HIPDHAIEYQ NLSVSCCVVK SDWILLQLIP NKTIGYRHGF TCVRFKHARA
     RRASARSYLA LNVDAHGRLC VCVIQQCFAA KCGNNKLRTL FTVDIDSKCR LEHQ