ID   PRIM_HHV6U              Reviewed;         860 AA.
AC   P52467;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   02-JUN-2021, entry version 65.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=U43;
OS   Human herpesvirus 6A (strain Uganda-1102) (HHV-6 variant A) (Human B
OS   lymphotropic virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Roseolovirus.
OX   NCBI_TaxID=10370;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=7747482; DOI=10.1006/viro.1995.1228;
RA   Gompels U.A., Nicholas J., Lawrence G.L., Jones M., Thomson B.J.,
RA   Martin M.E.D., Efstathiou S., Craxton M.A., Macaulay H.A.;
RT   "The DNA sequence of human herpesvirus-6: structure, coding content, and
RT   genome evolution.";
RL   Virology 209:29-51(1995).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; X83413; CAA58377.1; -; Genomic_DNA.
DR   EMBL; X92436; CAA63169.1; -; Genomic_DNA.
DR   RefSeq; NP_042936.1; NC_001664.2.
DR   PRIDE; P52467; -.
DR   DNASU; 1487921; -.
DR   GeneID; 1487921; -.
DR   KEGG; vg:1487921; -.
DR   Proteomes; UP000009295; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..860
FT                   /note="DNA primase"
FT                   /id="PRO_0000116108"
FT   ZN_FING         804..842
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            492
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            494
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   860 AA;  100080 MW;  660E8AE30B4674F6 CRC64;
     MTIVVFATEY DAANVIFSIL CRSPSEHLIF PIIVKYKPSN NVSFCLQTQK CKNSKRIDTV
     FVCHAEKLNL SHYIQTASPI KAEDVANSLN DKETESLYVD MILSQTGKER EDVEFKYMAY
     FHKSLIIKYL TGKFLLPTSP FWFLSTYGQT EGMLLLTMFY YLFEEQKSTI TTTKNYVQCF
     TENTGNMVFT YSSMSEFINI TLKSKFRKLF ADFSTYARQK NLRDKEEFKH LDTQINLFRK
     SSHLTNTFRV HYIYIAYNTA LETTKFVNYC NLTSYDSNLP IGQQCQRNLH ILGNSLHENL
     LCIMKQYFNA DCYFKTYIDI KRLKNPNLNV TEYEYALVSK KKTIQALTSE QITRAIAKCN
     KNGEGLFSPV KPGLQGLLEI SASDRYVQIQ DKRIYRRQHL HKDYHRPFPV FRVQLLHKNI
     FCFGNSEDWY ENMGFNRILQ YLPDEYISDE ALTRAIWLQD THFLCDDVEK QFYTTRHEIF
     NERIPVTNYI GDLDLPLQDT ATITEETFFS MCRLIRLTLI NAWKKIFPSI DTDTHPIFFF
     KTQCDTTNDT LDYTEDPTEI KQFCVCRKKI GLRISIPLPN GTAIAGGEPL KQLSKILNHV
     MCLDQELSQI LNSITFPGEC FDIGIYHTGH CIRIGYMYKT DMDKGKMLHG RLTPIFIVPE
     GYRNSCKTFI QMQMDLNNLL HHGTKKAPIE ELIYNITDKG CPKENLSFMD LKSRQLWNKV
     NIATETLITK YLNTHGFNNN ATSADDSLLS FIRLIGWPII KTQLITHYET RIAQQFSQVT
     FIKIDSKNLQ IKKTQFGRVS DFSCLNRQHR GNRDNVLVYI QLKADGNRLI LILWSTCFAT
     KCQSNSKQVH CSIALEQLKN