ID   PRIM_HHV6Z              Reviewed;         860 AA.
AC   P52540;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   02-JUN-2021, entry version 68.
DE   RecName: Full=DNA primase {ECO:0000255|HAMAP-Rule:MF_04011};
DE            EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_04011};
GN   Name=U43; Synonyms=KA4L;
OS   Human herpesvirus 6B (strain Z29) (HHV-6 variant B) (Human B lymphotropic
OS   virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Roseolovirus.
OX   NCBI_TaxID=36351;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7983761; DOI=10.1128/jvi.69.1.589-596.1995;
RA   Stamey F.R., Dominguez G., Black J.B., Dambaugh T.R., Pellett P.E.;
RT   "Intragenomic linear amplification of human herpesvirus 6B oriLyt suggests
RT   acquisition of oriLyt by transposition.";
RL   J. Virol. 69:589-596(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10482553; DOI=10.1128/jvi.73.10.8040-8052.1999;
RA   Dominguez G., Dambaugh T.R., Stamey F.R., Dewhurst S., Inoue N.,
RA   Pellett P.E.;
RT   "Human herpesvirus 6B genome sequence: coding content and comparison with
RT   human herpesvirus 6A.";
RL   J. Virol. 73:8040-8052(1999).
CC   -!- FUNCTION: Essential component of the helicase/primase complex. Unwinds
CC       the DNA at the replication forks and generates single-stranded DNA for
CC       both leading and lagging strand synthesis. The primase initiates primer
CC       synthesis and thereby produces large amount of short RNA primers on the
CC       lagging strand that the polymerase elongates using dNTPs.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBUNIT: Associates with the helicase and the primase-associated factor
CC       to form the helicase-primase factor. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-Rule:MF_04011}.
CC       Note=Requires the presence of the primase associated factor to properly
CC       localize in the host cell nucleus. {ECO:0000255|HAMAP-Rule:MF_04011}.
CC   -!- SIMILARITY: Belongs to the herpesviridae DNA primase family.
CC       {ECO:0000255|HAMAP-Rule:MF_04011}.
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DR   EMBL; AF157706; AAB06341.1; -; Genomic_DNA.
DR   PIR; T44003; T44003.
DR   RefSeq; NP_050224.1; NC_000898.1.
DR   PRIDE; P52540; -.
DR   DNASU; 1497045; -.
DR   GeneID; 1497045; -.
DR   KEGG; vg:1497045; -.
DR   Proteomes; UP000006930; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039686; P:bidirectional double-stranded viral DNA replication; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04011; HSV_PRIM; 1.
DR   InterPro; IPR033685; HSV_PRIM.
PE   3: Inferred from homology;
KW   DNA replication; Host nucleus; Metal-binding; Reference proteome;
KW   Transferase; Zinc; Zinc-finger.
FT   CHAIN           1..860
FT                   /note="DNA primase"
FT                   /id="PRO_0000116109"
FT   ZN_FING         804..842
FT                   /note="CHC2-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            492
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
FT   SITE            494
FT                   /note="Essential for primase activity"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04011"
SQ   SEQUENCE   860 AA;  100003 MW;  10BD77B1CFB0D8DF CRC64;
     MTIVVFATEY DAANVIFSIL CRSPSEHLIF PIIVKYKPSN NVSFCLQTQK CKNSKRIDTV
     FVCHAEKLNL SHYIQTASPI KAEDVANSLN DKETELLYVD MILSQTGKEK EDVEFKYMAY
     FHKSLIIKYL TGKFLLPTSP FWFLSTYGQT EGLLLLTMYY YLFEEQKSTI TTTKNYVQCF
     TENTGSMVFT YSSMSEFINI TLKSKFRKLF ADFATYARQK NLRDKEEFKY LDTQINLFRK
     SSHLTNTFRV HYIYIAYNTA LETTKFVNYC NITSYDSNLP IGQQCQRNVH ILGNSLHENL
     LCIMKQYFNA DCYFKTYIDI KRLKNPDLNV TEYEYALASK KKTIQALTSE QITRAIAKCN
     KNGEGLFSPV KPGLQGLLEI SASDKYVQIQ DKRIYRRQHL HKDYHRPFPV FRVQLLHKNI
     FCFGNSEDWY ENMGFNRILQ YLPDEYISDE ALTRAIWLQD THFLCDDFEK QFYTTRHEIF
     NERIPVTNYI GDLDLPLQDT ATITEETFFS MCRLIRLTLI NAWKKIFPSI DTHTHPIFFF
     KTQCDTTNDA LDYTEDPTEI KQFCICRKKI GLRISIPLPN GTAIAGGEPL KQLSKILNHV
     MCLDQELSQI LNSLTFPGEC FDIGIYHTGH CIRIGYMYKT DMDKGKMLHG RLTPIFIVPE
     GYRNSCKTFI QMQMDLNNLL HHGTKKAPIE ELIYSITDKG CPKENLSFMD LKSRQLWNKV
     NIATDTLITK YLNTHGFNNN ATSADDSLLS FIRLIGWPII KTQLITHYET RIAQQFSQVT
     FLKIDSKNLQ IKKTQFGRVS DFSCLNRQHR GNRDNVLVYI QLKADGNRLI LILWSTCFAT
     KCQSNSKQVH CSIALEQLKN